FastQCFastQC Report
Fri 10 Feb 2017
SRR3558331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345788
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314592.337589575772707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150761.12023587667597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120980.8989528811372965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54510.40504150728049293No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42900.31877234750198397No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT34670.2576185847993889No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31510.2341379177106647No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26280.19527592756065593No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23100.17164664865491447No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC23020.17105220138684546No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT21760.16168965691475923No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.1569340787702075No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA20270.1506180765469747No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT19390.14407915659821607No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18440.13702009528989706No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC18290.13590550666226775No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC17880.1328589644134143No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT16360.12156446632010391No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA15410.11450540501178492No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.10967552095872456No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG14350.10662897870987109No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.10261645965040556No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG13510.10038728239514694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4450.060.452521
TATCACG2200.059.5472762
TACAACG2400.049.6227232
TAACGCA3250.047.637824
GTATCAA187650.044.600671
GTATAAC4250.043.604841
TACTGGT10550.042.8966142
ATAGTAC6550.041.8194584
GCATAGT6700.041.771972
AGATTAC7750.039.95432
GTACTGG12500.039.216221
ACAACGC3100.038.417593
GGTTCAC11550.038.096226
GACCGTT3300.037.838817
CATAGTA7250.036.9603773
TATCAAC228550.036.6063962
ATCAACG231250.036.127493
TCAACGC237000.035.8791164
CAACGCA238800.035.8558735
AACGCAG244450.035.805516
Produced by FastQC (version 0.11.5)