##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3558320.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478214 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.105860556152685 32.0 32.0 32.0 32.0 32.0 2 31.67096320893993 32.0 32.0 32.0 32.0 32.0 3 35.13559410640425 37.0 32.0 37.0 32.0 37.0 4 36.03368575574952 37.0 37.0 37.0 32.0 37.0 5 36.23385764532197 37.0 37.0 37.0 37.0 37.0 6 39.27378328530742 41.0 41.0 41.0 37.0 41.0 7 39.34589535228998 41.0 41.0 41.0 37.0 41.0 8 40.20067375693727 41.0 41.0 41.0 37.0 41.0 9 40.24472934711238 41.0 41.0 41.0 37.0 41.0 10-11 40.347281760885295 41.0 41.0 41.0 41.0 41.0 12-13 40.00743600145542 41.0 41.0 41.0 37.0 41.0 14-15 39.34760902022944 41.0 41.0 41.0 37.0 41.0 16-17 39.32167293303835 41.0 41.0 41.0 37.0 41.0 18-19 38.18541176126169 41.0 37.0 41.0 29.5 41.0 20-21 38.16350734190132 41.0 37.0 41.0 29.5 41.0 22-23 38.499728155177394 41.0 37.0 41.0 32.0 41.0 24-25 38.427118403058046 41.0 37.0 41.0 32.0 41.0 26-27 38.207986382665496 41.0 37.0 41.0 29.5 41.0 28-29 37.98484674225347 41.0 37.0 41.0 27.0 41.0 30-31 37.702636267445115 41.0 37.0 41.0 27.0 41.0 32-33 37.27811084577198 41.0 37.0 41.0 27.0 41.0 34-35 36.76716281831983 41.0 37.0 41.0 27.0 41.0 36-37 36.32278854236806 41.0 37.0 41.0 22.0 41.0 38-39 35.85276152517492 41.0 37.0 41.0 22.0 41.0 40-41 35.39267252736222 41.0 37.0 41.0 12.0 41.0 42-43 35.06491863475348 41.0 32.0 41.0 12.0 41.0 44-45 34.69518249152053 41.0 32.0 41.0 12.0 41.0 46-47 34.270486644054756 41.0 32.0 41.0 12.0 41.0 48-49 34.09583889221143 41.0 32.0 41.0 12.0 41.0 50-51 34.0061018707106 41.0 29.5 41.0 12.0 41.0 52-53 33.96384463859276 41.0 27.0 41.0 12.0 41.0 54-55 33.79176791143714 41.0 27.0 41.0 12.0 41.0 56-57 33.68014947283015 41.0 27.0 41.0 12.0 41.0 58-59 33.504649592023654 41.0 27.0 41.0 12.0 41.0 60-61 33.45702656133028 41.0 27.0 41.0 12.0 41.0 62-63 33.37387968566374 41.0 27.0 41.0 12.0 41.0 64-65 33.33469743671243 41.0 27.0 41.0 12.0 41.0 66-67 33.15579217672423 41.0 27.0 41.0 12.0 41.0 68-69 32.980000585511924 41.0 27.0 41.0 12.0 41.0 70-71 32.876369156904644 41.0 27.0 41.0 12.0 41.0 72-73 32.580595716562044 41.0 24.5 41.0 12.0 41.0 74-75 32.23604285947296 41.0 22.0 41.0 12.0 41.0 76-77 31.44289167611153 39.0 22.0 41.0 12.0 41.0 78-79 31.422662239081248 39.0 22.0 41.0 12.0 41.0 80-81 31.60957123798132 41.0 22.0 41.0 12.0 41.0 82-83 31.540081427979942 41.0 22.0 41.0 12.0 41.0 84-85 31.404025185377257 41.0 22.0 41.0 12.0 41.0 86-87 31.278099344644865 41.0 17.0 41.0 12.0 41.0 88-89 31.1125050709515 41.0 12.0 41.0 12.0 41.0 90-91 31.01983839870853 41.0 12.0 41.0 12.0 41.0 92-93 30.88463951285408 41.0 12.0 41.0 12.0 41.0 94-95 30.842994977144123 41.0 12.0 41.0 12.0 41.0 96-97 30.760430476732175 41.0 12.0 41.0 12.0 41.0 98-99 30.685520499190737 41.0 12.0 41.0 12.0 41.0 100-101 30.53323825734922 41.0 12.0 41.0 12.0 41.0 102-103 30.410819214828507 41.0 12.0 41.0 12.0 41.0 104-105 30.21977712906774 37.0 12.0 41.0 12.0 41.0 106-107 30.16913452972937 37.0 12.0 41.0 12.0 41.0 108-109 30.096923134830853 37.0 12.0 41.0 12.0 41.0 110-111 29.98670260594629 37.0 12.0 41.0 12.0 41.0 112-113 29.87616109105965 37.0 12.0 41.0 12.0 41.0 114-115 29.670465523803152 37.0 12.0 41.0 12.0 41.0 116-117 29.33663903608008 37.0 12.0 41.0 12.0 41.0 118-119 28.9370470124254 37.0 12.0 41.0 12.0 41.0 120-121 28.69541983296181 34.5 12.0 41.0 12.0 41.0 122-123 28.43003027933101 32.0 12.0 41.0 12.0 41.0 124-125 27.607189459112448 29.5 12.0 41.0 12.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.09824134077835822 1101 2 0.015484284471497745 1101 3 -0.12272802276899597 1101 4 -0.137426956303635 1101 5 0.07823838529498772 1101 6 0.1456844575265137 1101 7 0.041612509097369355 1101 8 0.07059372237584682 1101 9 0.15080755704533289 1101 10-11 0.10477843691527511 1101 12-13 0.2225429560899741 1101 14-15 0.1841624973301279 1101 16-17 0.08880221881939576 1101 18-19 -0.30657356545410863 1101 20-21 -0.21588518643121546 1101 22-23 -0.009292182111096281 1101 24-25 -0.19274676537147428 1101 26-27 0.0764495041480231 1101 28-29 -0.3605680631782775 1101 30-31 -0.5481544475009272 1101 32-33 -0.32706980686027975 1101 34-35 -0.3068816871245019 1101 36-37 -1.0740920004431018 1101 38-39 -0.22951451154578706 1101 40-41 -0.6413900576052285 1101 42-43 -0.3142904984425243 1101 44-45 -0.3429721263469787 1101 46-47 0.22525985290408812 1101 48-49 -0.28384703220066854 1101 50-51 -0.0947074021479537 1101 52-53 -0.13677278304436413 1101 54-55 0.02158877828772887 1101 56-57 0.0157201263865403 1101 58-59 0.2904128059867972 1101 60-61 -0.17461865516003883 1101 62-63 -0.1568530209405523 1101 64-65 -0.06505411029757369 1101 66-67 -0.4579210225750501 1101 68-69 -0.4266582360257303 1101 70-71 -0.3718745572429256 1101 72-73 -0.5396286628213076 1101 74-75 -0.5853374814077021 1101 76-77 -0.03035846240663531 1101 78-79 -0.0619489483736686 1101 80-81 0.3298702472801196 1101 82-83 -0.15629065125609998 1101 84-85 -0.05278616086442511 1101 86-87 0.03759539979577298 1101 88-89 0.22715883733342324 1101 90-91 -0.35589591913482366 1101 92-93 -0.16672138109893808 1101 94-95 0.01897391421324457 1101 96-97 -0.04480974786819303 1101 98-99 0.13680444921485702 1101 100-101 0.5793270668548409 1101 102-103 0.5246163668688162 1101 104-105 0.8034547547030471 1101 106-107 0.7548218169688425 1101 108-109 0.356910069571029 1101 110-111 0.437518739750562 1101 112-113 0.5568399134165745 1101 114-115 0.8107559091552012 1101 116-117 0.41237828841086 1101 118-119 0.43087581415680987 1101 120-121 0.42458434723561567 1101 122-123 0.4785512532790044 1101 124-125 0.2862748885506683 1102 1 -0.28229979208792244 1102 2 -0.009060294490588205 1102 3 0.23785462618138098 1102 4 0.018539861911136768 1102 5 -0.11490777404799957 1102 6 0.13614624772732498 1102 7 -0.17128841158325514 1102 8 0.02623742208276525 1102 9 0.08956520780807864 1102 10-11 0.13276132820453768 1102 12-13 0.004880697781587173 1102 14-15 -0.06348401930736713 1102 16-17 0.03174125091376112 1102 18-19 -0.41599889090280584 1102 20-21 -0.4803789156537661 1102 22-23 0.017579989307414223 1102 24-25 -0.15969184558150573 1102 26-27 -0.02332110242412 1102 28-29 -0.4214557070022664 1102 30-31 -0.26556815311096926 1102 32-33 -0.2132792746468084 1102 34-35 0.12244649564458143 1102 36-37 -0.15416480567099455 1102 38-39 -0.07347519879400011 1102 40-41 -0.5016309465953839 1102 42-43 -0.6253386658841933 1102 44-45 -0.3906476407992798 1102 46-47 -0.4316235033858504 1102 48-49 -0.8397066620642732 1102 50-51 -0.535624354477612 1102 52-53 -0.3153112931780697 1102 54-55 -0.262196265053646 1102 56-57 -0.7883090164173367 1102 58-59 -0.6033153809248262 1102 60-61 -0.6664630197454144 1102 62-63 -0.594582802731999 1102 64-65 -0.62406207434028 1102 66-67 -0.5279248544291519 1102 68-69 -0.7036779856088842 1102 70-71 -0.49454308115058865 1102 72-73 -0.25636661578271003 1102 74-75 -0.0879524629215993 1102 76-77 -0.5717140066852657 1102 78-79 -0.5189752224651549 1102 80-81 -0.26964662841246323 1102 82-83 -0.4220841039637726 1102 84-85 -0.36402851010167936 1102 86-87 -0.36265988453815723 1102 88-89 -0.3338582610209464 1102 90-91 -0.8878168172385728 1102 92-93 -0.4133969887585067 1102 94-95 -0.4659006184558976 1102 96-97 -0.4875054054454253 1102 98-99 -0.5091555338007119 1102 100-101 -0.29531693318658014 1102 102-103 -0.9009317436128548 1102 104-105 -0.6367476086188617 1102 106-107 -0.582474551054851 1102 108-109 -0.5462165162469681 1102 110-111 -0.6022108556263568 1102 112-113 -0.44426821736356814 1102 114-115 -0.23886142325295978 1102 116-117 -0.3094653512291501 1102 118-119 -0.36360476248545126 1102 120-121 -0.6676157583992826 1102 122-123 -0.7376015652050292 1102 124-125 -0.3656503592253024 1103 1 0.10413257833652168 1103 2 -0.02157463383775493 1103 3 -0.12495692872528252 1103 4 -0.01733457755074852 1103 5 -0.35674738193203837 1103 6 0.043852995041959275 1103 7 -0.08828705586315522 1103 8 0.10983320892184878 1103 9 0.07691529645131823 1103 10-11 -0.038502620069529314 1103 12-13 0.15093868088722218 1103 14-15 0.13556697662546924 1103 16-17 0.3882382787581662 1103 18-19 -0.11678625142005927 1103 20-21 0.03123334839879277 1103 22-23 0.06397168812897291 1103 24-25 -0.061928783739809035 1103 26-27 0.1678615026441861 1103 28-29 0.029587154609998834 1103 30-31 0.02848232139106699 1103 32-33 0.17950412299632035 1103 34-35 0.33937213961162627 1103 36-37 0.5276320448869356 1103 38-39 0.4982528228975198 1103 40-41 0.054495865376672725 1103 42-43 -0.42302351310497244 1103 44-45 -0.12905623397896449 1103 46-47 0.12873211242392557 1103 48-49 0.13828895791695572 1103 50-51 0.7743349739195082 1103 52-53 0.5421868818141107 1103 54-55 0.15981854358125247 1103 56-57 0.1496720035013155 1103 58-59 0.5842067798844326 1103 60-61 0.48532548791961005 1103 62-63 0.18092559230397853 1103 64-65 0.20184260810835752 1103 66-67 0.24327130784011786 1103 68-69 -0.015629179685433314 1103 70-71 0.03405218425768908 1103 72-73 0.29418088656372987 1103 74-75 0.5423009524501836 1103 76-77 0.11236671080352423 1103 78-79 0.5266916875358838 1103 80-81 0.3074469162741593 1103 82-83 0.035951144758723075 1103 84-85 0.3628564622624886 1103 86-87 0.4798210832374217 1103 88-89 0.4959675810898041 1103 90-91 0.6639091187297232 1103 92-93 0.39536304878451034 1103 94-95 0.024119195914732217 1103 96-97 0.30095533081487247 1103 98-99 0.7331898619652755 1103 100-101 0.608088009977461 1103 102-103 0.5556330702363432 1103 104-105 0.5873529232405623 1103 106-107 0.6891335415512572 1103 108-109 0.8600197961943721 1103 110-111 1.0359370221502608 1103 112-113 0.9563001422151132 1103 114-115 0.8277305049981578 1103 116-117 0.8941898787218356 1103 118-119 0.7157622205159058 1103 120-121 0.7184776820011791 1103 122-123 0.6674980280789384 1103 124-125 0.42022408023658286 1104 1 0.036706682961078485 1104 2 0.13275456631125238 1104 3 -0.08166492723520236 1104 4 0.04445031947442146 1104 5 -0.43039966227900806 1104 6 -0.07311789527787482 1104 7 -0.009930395759916166 1104 8 0.09996678350542254 1104 9 0.06926269680254649 1104 10-11 0.1611807389853368 1104 12-13 0.19177205856803425 1104 14-15 0.250206963853401 1104 16-17 0.340114176794458 1104 18-19 0.12253842528686931 1104 20-21 -0.0160712122951594 1104 22-23 -0.11227758013774292 1104 24-25 -0.08634472754269495 1104 26-27 -0.04042970059141027 1104 28-29 0.06539152904457524 1104 30-31 0.37151355974985023 1104 32-33 -0.003886862049711226 1104 34-35 0.39025288039178463 1104 36-37 0.06781085389124542 1104 38-39 -0.006600564362337025 1104 40-41 0.1241142997335487 1104 42-43 0.08503885717868087 1104 44-45 -0.057644387882433534 1104 46-47 -0.22323159360555422 1104 48-49 -0.39565016187442836 1104 50-51 -0.30771494359419194 1104 52-53 -0.5756062106237607 1104 54-55 -0.3142275423109311 1104 56-57 -0.35452947593032746 1104 58-59 -0.17779896754785085 1104 60-61 -0.14494964379237985 1104 62-63 -0.2643886538777025 1104 64-65 -0.5056051062229727 1104 66-67 -0.46978281381386466 1104 68-69 -0.37591069743328376 1104 70-71 -0.2038303126823351 1104 72-73 0.12387063165446222 1104 74-75 -0.2741537333036348 1104 76-77 -0.5654364928578026 1104 78-79 -0.4566567239376944 1104 80-81 -0.351618593810457 1104 82-83 -0.47906402207646437 1104 84-85 -0.1343310211072115 1104 86-87 -0.49453898275161734 1104 88-89 -0.43634309955518447 1104 90-91 -0.29683006492202324 1104 92-93 0.05884290616304355 1104 94-95 -0.03735704271013773 1104 96-97 -0.1830370059420865 1104 98-99 -0.14868976125861266 1104 100-101 0.0473142352980922 1104 102-103 -0.3970836558559334 1104 104-105 0.04464098667518357 1104 106-107 -0.49385565540472953 1104 108-109 0.05763567613689702 1104 110-111 0.19207293828032235 1104 112-113 -0.1300949405372691 1104 114-115 0.2942064775382036 1104 116-117 6.956527625980868E-4 1104 118-119 0.1257616883462518 1104 120-121 0.2335114747743141 1104 122-123 0.5102152863408733 1104 124-125 0.6502011969413886 1105 1 -0.04050462022408752 1105 2 -0.16927322130217348 1105 3 0.01584010656125656 1105 4 -0.033500859305945596 1105 5 0.008954320629861456 1105 6 -0.13145180113399135 1105 7 -0.24476162485182584 1105 8 0.0036191265328895383 1105 9 0.10692764942408672 1105 10-11 0.004316543150842733 1105 12-13 0.0989863741038306 1105 14-15 0.3954788945587637 1105 16-17 0.06917173383094166 1105 18-19 0.030608882582832564 1105 20-21 -0.18239788850974037 1105 22-23 -0.21252543846213712 1105 24-25 -0.24470981387032253 1105 26-27 -0.21685303626767904 1105 28-29 -0.21622626179259896 1105 30-31 -0.008893477524019033 1105 32-33 0.2329763825161706 1105 34-35 0.420600991859331 1105 36-37 0.4917278609887674 1105 38-39 -0.16253991570283688 1105 40-41 0.20618500013149088 1105 42-43 0.009035136661402987 1105 44-45 -0.11202524412988168 1105 46-47 -0.297834835317623 1105 48-49 -0.05636435321682853 1105 50-51 -0.0693032451040736 1105 52-53 -0.3688743996725421 1105 54-55 -0.07425417783235844 1105 56-57 0.34597416795697455 1105 58-59 0.00865760968195417 1105 60-61 -0.08223990227320144 1105 62-63 0.2600061014612933 1105 64-65 0.1982253354299104 1105 66-67 0.561709462413404 1105 68-69 0.888584258200602 1105 70-71 0.4930215166600789 1105 72-73 0.095475263275695 1105 74-75 0.39387729919275927 1105 76-77 0.2987408447527109 1105 78-79 0.3087207975847619 1105 80-81 0.2455746352708843 1105 82-83 0.01922897922888822 1105 84-85 0.3248119915605159 1105 86-87 0.46138292866413266 1105 88-89 0.6082211929916248 1105 90-91 0.10253364206610271 1105 92-93 0.5850107205176869 1105 94-95 0.5074265701023926 1105 96-97 0.7669685431248787 1105 98-99 0.499391054295689 1105 100-101 0.4361399998802469 1105 102-103 0.35487040843925755 1105 104-105 0.6071499048185203 1105 106-107 1.2906185837124902 1105 108-109 0.5682264667996648 1105 110-111 0.3197358298198445 1105 112-113 0.25776370094544276 1105 114-115 0.5913563710489669 1105 116-117 0.49205496277575733 1105 118-119 0.9932314723554256 1105 120-121 1.1878637929630962 1105 122-123 1.4981817382674585 1105 124-125 0.9537113781580615 1106 1 0.09125882418370068 1106 2 0.12636971832171184 1106 3 0.08886159286940654 1106 4 0.20141542244925148 1106 5 0.21265668783540548 1106 6 -0.11561544349624597 1106 7 0.017693010582021884 1106 8 -0.16682792064625573 1106 9 -0.09278985305034837 1106 10-11 -0.05358256192999278 1106 12-13 0.08515275896031937 1106 14-15 0.04795796499756477 1106 16-17 0.12499077045251283 1106 18-19 -0.10689214843002759 1106 20-21 -0.007860297780609926 1106 22-23 -0.2760719165221843 1106 24-25 -0.1029223468627336 1106 26-27 -0.04416611363488698 1106 28-29 -0.10573261283185786 1106 30-31 -0.24071929821026572 1106 32-33 0.1826758422654251 1106 34-35 0.1597573140302302 1106 36-37 0.24129483558461118 1106 38-39 0.13945921824635832 1106 40-41 -0.13950330405854316 1106 42-43 0.23654874603123943 1106 44-45 0.8815386580476172 1106 46-47 0.8938639645833959 1106 48-49 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48-49 0.99776416844621 2225 50-51 1.2286431552813966 2225 52-53 1.4557471963531796 2225 54-55 0.900502275665346 2225 56-57 1.1444882435325425 2225 58-59 1.127185420227967 2225 60-61 0.8556896616968714 2225 62-63 0.9617855554962134 2225 64-65 1.11260330187892 2225 66-67 0.6493557426178782 2225 68-69 0.9049803791791078 2225 70-71 1.0568032857021166 2225 72-73 0.9572615847958375 2225 74-75 1.091043353877886 2225 76-77 1.2833499242327768 2225 78-79 1.1957229741345152 2225 80-81 1.5534733509403793 2225 82-83 1.1175890902163097 2225 84-85 1.4329222700813453 2225 86-87 1.4456790945028253 2225 88-89 1.2542604816530734 2225 90-91 1.0943508118868301 2225 92-93 -2.9599740768638085 2225 94-95 0.2934739446738668 2225 96-97 1.4434906049211698 2225 98-99 1.5445835377223425 2225 100-101 1.7267093919416574 2225 102-103 1.583685047259376 2225 104-105 1.4722952019757116 2225 106-107 1.2816520848561481 2225 108-109 1.285283306316508 2225 110-111 0.82370401785602 2225 112-113 1.028691531429324 2225 114-115 1.35614777118521 2225 116-117 1.6824532215359582 2225 118-119 1.5360064902164225 2225 120-121 1.0444773473851505 2225 122-123 1.4861082561755339 2225 124-125 1.2139890682461747 2226 1 0.03152090023714038 2226 2 -0.16434413916372748 2226 3 -0.21092536327996214 2226 4 -0.18040412191525945 2226 5 -0.15173929537802167 2226 6 0.06287033930608033 2226 7 -0.03489349164476607 2226 8 -0.17483580005701782 2226 9 -0.7231962422247236 2226 10-11 -0.47937317943598856 2226 12-13 -0.41465721448949466 2226 14-15 -0.315707352748241 2226 16-17 -0.323172127800305 2226 18-19 -0.5334027256825138 2226 20-21 -0.4839303346082957 2226 22-23 -0.4061303697595946 2226 24-25 -0.4828924779007622 2226 26-27 -0.47078107166846195 2226 28-29 -0.21087350012202677 2226 30-31 -0.2761632644658292 2226 32-33 -0.4348007122052877 2226 34-35 0.039422332015845996 2226 36-37 0.2123679770714233 2226 38-39 0.711364194265542 2226 40-41 0.7480545068356008 2226 42-43 0.7288085096489212 2226 44-45 -0.0857848046108316 2226 46-47 0.26833670781738306 2226 48-49 -0.07417827759695683 2226 50-51 0.17828344305117838 2226 52-53 0.24831314359538226 2226 54-55 0.32496270732002586 2226 56-57 -0.5305717085058248 2226 58-59 0.006082302721971189 2226 60-61 0.18422683196066458 2226 62-63 -0.1964878257987479 2226 64-65 0.10540905727460625 2226 66-67 0.1985164140807072 2226 68-69 0.35102354464673624 2226 70-71 0.7498488492512791 2226 72-73 -0.15065208426890564 2226 74-75 0.043081723182432086 2226 76-77 -0.22504336113892265 2226 78-79 0.041046715141707324 2226 80-81 0.2441208329547706 2226 82-83 0.19073057702686214 2226 84-85 0.3825625578511307 2226 86-87 0.3005951616491096 2226 88-89 0.002461920501996673 2226 90-91 0.5092189174024142 2226 92-93 -1.8856335492858705 2226 94-95 -0.07942773398320924 2226 96-97 0.24444983753507543 2226 98-99 0.47503917321394695 2226 100-101 0.5048868499752288 2226 102-103 0.1880015940219657 2226 104-105 0.5118635472994555 2226 106-107 0.3308127563189771 2226 108-109 0.2996717955251391 2226 110-111 0.5599150490310798 2226 112-113 0.402792250853782 2226 114-115 0.4160998095545132 2226 116-117 -0.030976035058767337 2226 118-119 -0.10188319803297574 2226 120-121 -0.20612217299854407 2226 122-123 0.1863480643290103 2226 124-125 -0.21167040417588723 2227 1 0.27127367076898423 2227 2 0.027031628582353306 2227 3 0.050482138893819695 2227 4 0.10506727002430694 2227 5 0.06669104118413571 2227 6 0.24730108570203413 2227 7 0.14322913727288267 2227 8 0.0867600041310439 2227 9 -0.06166357459439098 2227 10-11 0.14172993807000722 2227 12-13 0.05626120089367248 2227 14-15 -0.01676129019874395 2227 16-17 0.17078836200646208 2227 18-19 0.32160195417637283 2227 20-21 0.3557083470560727 2227 22-23 0.24359465391661672 2227 24-25 -0.09459434042921089 2227 26-27 -0.5655828284154865 2227 28-29 -0.6123001647949096 2227 30-31 0.2058871229378738 2227 32-33 0.1798585996062343 2227 34-35 0.23122454844131823 2227 36-37 0.42475326514581013 2227 38-39 -0.0759348348714255 2227 40-41 -0.32845703220107225 2227 42-43 0.1834000327352996 2227 44-45 -0.09932316560794874 2227 46-47 0.5197171277308783 2227 48-49 0.6156910740348991 2227 50-51 0.21360668561187168 2227 52-53 0.17385077353762313 2227 54-55 0.48117307389973973 2227 56-57 0.05151689313019148 2227 58-59 0.5063481408597781 2227 60-61 0.9200695665489675 2227 62-63 0.9874887804451262 2227 64-65 0.843721871226677 2227 66-67 1.3677140813510462 2227 68-69 0.38281058614516894 2227 70-71 0.536901424281325 2227 72-73 0.49039443186691045 2227 74-75 0.6560020105091624 2227 76-77 0.3795722535286963 2227 78-79 0.15745058973256576 2227 80-81 -0.005002731934659721 2227 82-83 0.09544145036052853 2227 84-85 0.30056256892772026 2227 86-87 0.26969701642395094 2227 88-89 -0.14812984626934522 2227 90-91 0.42701401158111096 2227 92-93 0.041130812896440716 2227 94-95 0.2695512814187886 2227 96-97 -0.0032393090691158477 2227 98-99 0.06174671249430119 2227 100-101 0.13013538094260113 2227 102-103 0.2959789534738988 2227 104-105 0.4143092581672505 2227 106-107 0.30793959987877173 2227 108-109 -0.021842817264996484 2227 110-111 0.2573801716212323 2227 112-113 0.2877867956336644 2227 114-115 0.024381775206013145 2227 116-117 0.44240138309908517 2227 118-119 0.4608989088450315 2227 120-121 0.47192845809011885 2227 122-123 0.04437111471087718 2227 124-125 0.45602084698023404 2228 1 0.41056875154447425 2228 2 0.08513182485924276 2228 3 -0.049417765484108145 2228 4 0.038355495088474356 2228 5 0.04524597354969018 2228 6 0.12893661461512806 2228 7 0.12544119460139314 2228 8 0.11374108661766513 2228 9 -0.04005074893412086 2228 10-11 -0.03260420236582462 2228 12-13 0.17468438728961644 2228 14-15 0.023612474682792595 2228 16-17 0.06309609732902999 2228 18-19 -0.042916664168529906 2228 20-21 0.11802877606926643 2228 22-23 -1.7319965533260557 2228 24-25 -0.1491352705915432 2228 26-27 -0.043693202012612176 2228 28-29 0.18005733874198881 2228 30-31 -0.09288712508677577 2228 32-33 0.1616596497714795 2228 34-35 0.20343543146364595 2228 36-37 0.3373904772310965 2228 38-39 0.11535964197517501 2228 40-41 0.7953483908567094 2228 42-43 0.4761825232709569 2228 44-45 0.1692770055052364 2228 46-47 0.48664922487395046 2228 48-49 0.11515984028645931 2228 50-51 0.554012883711728 2228 52-53 0.2696461982170604 2228 54-55 -0.0019980001430610628 2228 56-57 0.020551557530261277 2228 58-59 0.04233223739893077 2228 60-61 0.3185368173863665 2228 62-63 0.4181236327341864 2228 64-65 0.5163113281939502 2228 66-67 0.275536638095204 2228 68-69 0.6196289992660837 2228 70-71 0.6192230835200405 2228 72-73 0.5251510988504506 2228 74-75 0.5128792507777931 2228 76-77 0.15764516116671956 2228 78-79 0.5553306874795787 2228 80-81 0.3021538254469718 2228 82-83 0.2014812984835963 2228 84-85 -0.07173163391409787 2228 86-87 0.36741047453197595 2228 88-89 0.06554075525501801 2228 90-91 0.15364671656744022 2228 92-93 -0.17606270717021744 2228 94-95 -0.5050378512162972 2228 96-97 -0.29050815248936956 2228 98-99 -0.404830296827452 2228 100-101 -0.28278422381948687 2228 102-103 -0.06517891242033613 2228 104-105 0.15335326109742198 2228 106-107 0.13947139841256728 2228 108-109 -0.26194089979076196 2228 110-111 -0.4382704219625815 2228 112-113 -0.13092605295353366 2228 114-115 -0.03147046352484395 2228 116-117 0.14852007749428608 2228 118-119 -0.006958653407174609 2228 120-121 0.18728223180691117 2228 122-123 0.48657567884756503 2228 124-125 0.34395289379590466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 5.0 16 20.0 17 119.0 18 448.0 19 1269.0 20 3323.0 21 7764.0 22 13998.0 23 19407.0 24 22978.0 25 24137.0 26 23372.0 27 20683.0 28 16893.0 29 13338.0 30 10244.0 31 8546.0 32 7928.0 33 7896.0 34 9648.0 35 13268.0 36 17777.0 37 26361.0 38 46620.0 39 107213.0 40 54957.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.947292764734065 16.29069679469397 12.008759320438134 16.75325112013383 2 14.336468610287445 22.928647007406727 42.428494356083256 20.30639002622257 3 20.17820473679148 37.65197171140954 26.680942005043768 15.488881546755218 4 9.723281243453851 18.284595081486447 37.46931165947463 34.52281201558507 5 9.43803401824288 37.80064991823745 33.299108767204636 19.46220729631504 6 21.886854002601346 36.12190358291476 22.996817324461432 18.99442509002246 7 19.690766058710118 36.52193369495665 25.598790499650786 18.188509746682445 8 25.737013136378273 25.327363899844002 26.134533911596066 22.80108905218166 9 28.53283258122932 12.637020246165942 26.269828988695437 32.560318183909295 10-11 21.455143513155196 25.711396989632256 29.48993546822134 23.343524028991204 12-13 26.13970830410066 24.825623400916136 33.708975362999226 15.325692931983978 14-15 23.206548560052866 20.389910750880365 39.89956337356654 16.503977315500237 16-17 14.872630245036742 23.638266550121912 38.71969453006395 22.7694086747774 18-19 15.53676805781512 17.915723922762613 50.075280104722985 16.47222791469928 20-21 18.01641106282961 15.891420995621209 50.725616564968824 15.36655137658036 22-23 16.905646646996214 18.170355050991922 48.84936210433426 16.074636197677602 24-25 15.74267115281266 18.445232563732063 49.58271732470677 16.22937895874851 26-27 16.749163529903807 16.880489335006274 49.96580928481807 16.404537850271854 28-29 16.449886033332636 17.162125426068044 50.31189226490454 16.07609627569478 30-31 17.970406554387786 16.57531983588937 50.24570589735976 15.208567712363083 32-33 14.693676974148524 17.168413623650576 50.84026452675327 17.29764487544763 34-35 14.029194073358447 18.200448126203046 50.06017186884101 17.710185931597497 36-37 16.399119981931186 18.00586399872849 48.281685251332156 17.313330768008164 38-39 16.575005593765095 16.872468429208027 48.22379660861001 18.32872936841687 40-41 15.145663145546044 17.8591182316537 48.24151339527968 18.753705227520573 42-43 16.419825243591447 17.07790639438655 47.71914342802355 18.783124933998455 44-45 16.89328164915337 16.971079794336838 47.10740431298696 19.028234243522835 46-47 15.600628592160582 16.861995163227984 48.10758419653229 19.429792048079143 48-49 15.907208906472833 17.131765276633473 48.16985700962331 18.791168807270388 50-51 13.745947002847162 17.941511369303978 48.48937307021919 19.82316855762967 52-53 13.970105433968893 17.605611713584295 47.219654798897565 21.20462805354925 54-55 14.484519482909326 17.879861317318188 46.831648592471154 20.803970607301334 56-57 15.357567431078992 17.141509858106417 46.488000577151695 21.012922133662894 58-59 14.613798569244828 17.85986880213463 47.29375649559084 20.2325761330297 60-61 15.610479827023049 17.71926376057581 46.34807847532695 20.322177937074194 62-63 14.447088541949837 17.929107052491144 46.61668207120662 21.007122334352403 64-65 13.928115112848147 18.665269787896086 45.852441087474304 21.55417401178146 66-67 14.77200488486278 18.78685603874432 45.22157536824672 21.21956370814618 68-69 15.251284785938719 18.91968192725733 44.89217208550949 20.93686120129446 70-71 14.4584107918438 19.223755941390134 44.709052242410806 21.608781024355256 72-73 15.288689349724912 18.960902359408884 44.923600989517226 20.826807301348982 74-75 15.672915967449686 18.42743879041474 44.76442007597025 21.135225166165323 76-77 14.643520701614243 18.68472422766914 44.479168736009065 22.19258633470755 78-79 14.809490102017236 18.339577188372733 44.675959084364706 22.174973625245322 80-81 14.231703797881284 18.971841058605563 43.79786037213465 22.998594771378507 82-83 14.260187678071986 18.91721776406932 42.84862900907024 23.973965548788456 84-85 15.212202132320114 18.51837523773477 42.4030998836289 23.86632274631622 86-87 15.218082281154462 18.226463466146956 41.924535877243244 24.63091837545534 88-89 15.74265955956312 18.2591430071078 41.69575100322664 24.302446430102446 90-91 16.25585013613722 18.288393413551823 41.16645127291434 24.28930517739662 92-93 15.763531860922992 19.16046888772479 40.28174669146824 24.79425255988398 94-95 15.060242853513095 19.146593557574977 41.02871351211183 24.764450076800095 96-97 15.40858276838403 19.114141367672214 40.57890400531979 24.898371858623964 98-99 16.06315184149306 19.564419583344225 40.1439736518807 24.228454923282015 100-101 15.753930248800746 19.205941273153858 40.43409435942904 24.606034118616353 102-103 15.409815495454046 20.402783514317534 40.39943744150908 23.787963548719343 104-105 15.587325990708303 20.69439893809176 40.71365862674429 23.004616444455646 106-107 14.785418988689095 21.747199457979846 40.257124992942444 23.210256560388615 108-109 14.77762001451236 22.421075964057856 40.02055569380172 22.780748327628064 110-111 14.568829403461075 23.255084435614577 40.01338422125791 22.16270193966644 112-113 13.706963161543214 24.307024431760556 39.387339649254145 22.598672757442085 114-115 14.007237353190574 23.7276864831294 39.75546487081607 22.509611292863962 116-117 14.312884115522712 23.982819227576226 39.38832184254165 22.315974814359414 118-119 14.240753500929015 25.03772000723555 39.05774742285828 21.663779068977163 120-121 14.242174258986834 25.204119080703492 39.671715666747176 20.8819909935625 122-123 13.70506839202926 26.317913671923193 39.147405159776575 20.829612776270967 124-125 13.19712513644519 27.304303094430526 39.16614737335168 20.332424395772602 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 138.0 1 242.0 2 990.5 3 1923.0 4 2305.0 5 2679.5 6 3544.5 7 5616.0 8 9256.0 9 11919.5 10 12027.0 11 11280.0 12 10718.0 13 10209.0 14 9607.5 15 8957.0 16 8246.0 17 7516.5 18 6758.5 19 5982.0 20 5372.0 21 4942.0 22 4498.5 23 4081.5 24 3713.0 25 3290.0 26 2901.0 27 2627.0 28 2464.5 29 2443.5 30 2550.5 31 2613.0 32 3013.5 33 3323.0 34 3299.5 35 3510.0 36 3820.0 37 4557.5 38 4874.5 39 4297.5 40 3687.0 41 3318.0 42 3369.5 43 4059.5 44 5500.0 45 7197.5 46 9574.5 47 11047.5 48 13473.5 49 17298.5 50 22170.0 51 25423.0 52 25283.0 53 25351.0 54 23418.0 55 19519.0 56 15758.5 57 12087.0 58 8800.0 59 6344.0 60 4377.5 61 3165.5 62 2227.5 63 1386.0 64 767.0 65 385.5 66 244.0 67 165.0 68 134.0 69 114.0 70 87.0 71 70.0 72 68.0 73 61.5 74 51.5 75 37.5 76 38.0 77 31.5 78 21.5 79 11.0 80 7.0 81 7.5 82 4.5 83 2.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 1.5 98 2.0 99 4.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49726691397575146 2 0.0 3 0.0 4 0.17439890927492713 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002195669721087212 14-15 4.182228040166118E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.091114020083059E-4 24-25 2.091114020083059E-4 26-27 0.0029275596281162827 28-29 8.364456080332236E-4 30-31 0.0 32-33 3.1366710301245886E-4 34-35 1.0455570100415295E-4 36-37 0.008782678884348848 38-39 6.273342060249177E-4 40-41 9.410013090373765E-4 42-43 0.0011501127110456824 44-45 0.011187460007444366 46-47 5.227785050207648E-4 48-49 0.0 50-51 0.0038685609371536594 52-53 0.0 54-55 0.0 56-57 7.318899070290707E-4 58-59 0.0010455570100415296 60-61 0.0 62-63 0.0 64-65 0.0010455570100415296 66-67 4.182228040166118E-4 68-69 0.004495895143178577 70-71 2.091114020083059E-4 72-73 2.091114020083059E-4 74-75 1.0455570100415295E-4 76-77 5.227785050207648E-4 78-79 0.002195669721087212 80-81 0.0 82-83 3.1366710301245886E-4 84-85 5.227785050207648E-4 86-87 0.0 88-89 0.0014637798140581413 90-91 0.004182228040166118 92-93 0.0038685609371536594 94-95 0.005123229349203495 96-97 0.0 98-99 3.1366710301245886E-4 100-101 0.0 102-103 0.008678123183344695 104-105 0.43704283019735934 106-107 6.273342060249177E-4 108-109 2.091114020083059E-4 110-111 0.00815534467832393 112-113 9.410013090373765E-4 114-115 1.0455570100415295E-4 116-117 0.0010455570100415296 118-119 0.004077672339161965 120-121 1.0455570100415295E-4 122-123 0.0031366710301245883 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 478214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.286562507590588 #Duplication Level Percentage of deduplicated Percentage of total 1 76.20626238572721 20.794069420579948 2 10.310434527309232 5.626726324196872 3 4.165183088411326 3.409605860924846 4 2.1247433786041596 2.3190777205148643 5 1.3135472283543403 1.7921094276581542 6 0.8846716536566098 1.4483789025716771 7 0.6405373711792641 1.2234644111991524 8 0.4805359061197402 1.048973843158238 9 0.3851837680586825 0.9459306867638264 >10 2.847705984582024 15.18397355458066 >50 0.31905971680927114 6.086468020301744 >100 0.27139296393656076 14.456226907903119 >500 0.033828018167729956 6.256393195100779 >1k 0.0130699161102593 6.433660815724464 >5k 0.0015376371894422708 2.867539770775326 >10k+ 0.002306455784163406 10.107401138046326 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25238 5.277553563885625 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12341 2.580643812184503 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10601 2.216789972690051 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8082 1.6900383510311285 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5587 1.1683054030204052 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3980 0.8322633799930574 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3898 0.8151162450283763 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2208 0.4617179756343394 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2124 0.44415261786564175 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1914 0.40023922344389745 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1840 0.3847649796952829 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 1739 0.36364472809244397 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1715 0.3586260544442446 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1561 0.32642289853496553 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1527 0.3193131108666831 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 1371 0.2866917321533874 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1327 0.27749083046502193 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 1258 0.2630621437264488 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 1105 0.23106809921917804 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1065 0.22270364313884577 No Hit GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1027 0.21475740986253017 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1009 0.21099340462638067 No Hit GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1000 0.2091114020083059 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 980 0.20492917396813976 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 954 0.19949227751592385 No Hit GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.19907405471190723 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 930 0.1944736038677245 No Hit CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 886 0.18527270217935904 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 825 0.17251690665685238 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 803 0.16791645581266965 No Hit GTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 792 0.16561623039057827 No Hit CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 787 0.16457067338053674 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 771 0.16122489094840387 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 768 0.16059755674237894 No Hit GCAGTGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 761 0.15913377692832079 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 759 0.1587155541243042 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 752 0.15725177431024606 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 737 0.15411510328012146 No Hit GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC 725 0.15160576645602178 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG 720 0.15056020944598025 No Hit GTATAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.14972376383794703 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 695 0.1453324243957726 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA 686 0.14345042177769787 No Hit GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 638 0.13341307448129916 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 608 0.12713973242104998 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 607 0.1269306210190417 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC 599 0.12525772980297523 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 599 0.12525772980297523 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT 595 0.12442128419494201 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 587 0.12274839297887556 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 581 0.12149372456682574 No Hit CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 576 0.1204481675567842 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA 575 0.1202390561547759 No Hit GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC 563 0.11772971933067622 No Hit GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA 556 0.11626593951661807 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 542 0.1133383798885018 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.1131292684864935 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 539 0.11271104568247688 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 527 0.11020170885837721 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 520 0.10873792904431906 No Hit GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACGGACT 519 0.10852881764231077 No Hit GTATTAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 517 0.10811059483829416 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 517 0.10811059483829416 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 513 0.10727414923026093 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA 503 0.10518303521017787 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG 502 0.10497392380816957 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC 492 0.10288280978808649 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 481 0.10058258436599515 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.182228040166118E-4 0.0 6 0.0 0.0 0.0 4.182228040166118E-4 0.0 7 0.0 0.0 0.0 4.182228040166118E-4 0.0 8 0.0 0.0 0.0 4.182228040166118E-4 0.0 9 0.0 0.0 0.0 4.182228040166118E-4 0.0 10-11 0.0 0.0 0.0 9.410013090373765E-4 0.0 12-13 0.0 0.0 0.0 0.0016728912160664472 0.0 14-15 0.0 0.0 0.0 0.0016728912160664472 0.0 16-17 0.0 0.0 0.0 0.0025093368240996705 0.0 18-19 0.0 0.0 0.0 0.004705006545186883 0.0 20-21 0.0 0.0 0.0 0.014742353841585565 0.0 22-23 0.0 0.0 0.0 0.04694550975086467 0.0 24-25 0.0 0.0 0.0 0.1275579552250666 0.0 26-27 0.0 0.0 0.0 0.23002254220913648 0.0 28-29 0.0 0.0 0.0 0.410172015039292 0.0 30-31 0.0 0.0 0.0 0.6928906305545216 0.0 32-33 0.0 0.0 0.0 1.1489625983346368 0.0 34-35 0.0 0.0 0.0 1.5916514363862204 0.0 36-37 0.0 0.0 0.0 2.154892997695592 0.0 38-39 0.0 0.0 0.0 2.8188216990719637 0.0 40-41 0.0 0.0 0.0 3.876716281831983 0.0 42-43 0.0 0.0 0.0 4.778090980188786 0.0 44-45 0.0 0.0 0.0 5.488546968512004 0.0 46-47 0.0 0.0 0.0 6.158539900546618 0.0 48-49 0.0 0.0 0.0 6.748024942808032 0.0 50-51 0.0 0.0 0.0 7.281781796434233 0.0 52-53 0.0 0.0 0.0 7.9507291714588035 0.0 54-55 0.0 0.0 0.0 8.553074564943728 0.0 56-57 0.0 0.0 0.0 9.10648789035871 0.0 58-59 0.0 0.0 0.0 9.744591333587056 0.0 60-61 0.0 0.0 0.0 10.294031542363879 0.0 62-63 0.0 0.0 0.0 10.838557633193506 0.0 64-65 0.0 0.0 0.0 11.40305386291493 0.0 66-67 0.0 0.0 0.0 11.931373820088915 0.0 68-69 0.0 0.0 0.0 12.486773703822974 0.0 70-71 0.0 0.0 0.0 13.132091490420606 0.0 72-73 0.0 0.0 0.0 13.677349471157264 0.0 74-75 0.0 0.0 0.0 14.217902445348734 0.0 76-77 0.0 0.0 0.0 14.797559251715759 0.0 78-79 0.0 0.0 0.0 15.347835906100617 0.0 80-81 0.0 0.0 0.0 15.904072235442712 0.0 82-83 0.0 0.0 0.0 16.556604365409626 0.0 84-85 0.0 0.0 0.0 17.10018945493022 0.0 86-87 0.0 0.0 0.0 17.670540803907873 0.0 88-89 0.0 0.0 0.0 18.199069872483868 0.0 90-91 0.0 0.0 0.0 18.71724792666045 0.0 92-93 0.0 0.0 0.0 19.190153362302233 0.0 94-95 0.0 0.0 0.0 19.67633737197154 0.0 96-97 0.0 0.0 0.0 20.16011660051776 0.0 98-99 0.0 0.0 0.0 20.664493302161794 0.0 100-101 0.0 0.0 0.0 21.235785652448484 0.0 102-103 0.0 0.0 0.0 21.73796668437143 0.0 104-105 0.0 0.0 0.0 22.21902746469154 0.0 106-107 0.0 0.0 0.0 22.671858205740527 0.0 108-109 0.0 0.0 0.0 23.1671385613972 0.0 110-111 0.0 0.0 0.0 23.642344222461077 0.0 112-113 0.0 0.0 0.0 24.152157820557324 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAC 155 0.0 100.39057 1 TACAACG 155 0.0 99.86502 2 ATAACGC 205 0.0 95.8367 3 GTATAAC 225 0.0 90.43732 1 GTGTTAA 20 8.284189E-4 89.77234 1 TCACGCA 130 0.0 87.01257 4 GGTTTCA 330 0.0 85.23839 1 TAACGCA 260 0.0 77.853355 4 CGCATAG 365 0.0 72.145805 1 TATGCGA 25 0.0020513302 71.4419 4 ATCATCG 75 3.6379788E-12 71.4419 3 TGAGTAC 75 3.6379788E-12 71.344795 5 GGGATTG 25 0.0020624287 71.344795 7 ATCTACG 120 0.0 69.457405 2 AGTGTAC 240 0.0 69.457405 3 AACTCAG 95 0.0 68.93517 2 TATCACG 165 0.0 68.55536 2 TATATGC 35 9.12042E-5 68.03991 2 GTTTCAA 425 0.0 67.23943 2 GAGTTCT 265 0.0 65.494286 1 >>END_MODULE