FastQCFastQC Report
Fri 10 Feb 2017
SRR3558295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156272
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT49183.147076891573666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26681.7072796150302036No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT19931.275340432067165RNA PCR Primer, Index 26 (100% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.847880618408928No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT13000.8318828708917784RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG10240.655267738302447RNA PCR Primer, Index 26 (100% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.5144875601515306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.347471076072489No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.2930787345141804No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT4440.28411999590457665RNA PCR Primer, Index 26 (100% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC4110.2630029691819392RNA PCR Primer, Index 26 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.20669089792157264No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT2870.18365414149687723No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG2770.1772550424900174RNA PCR Primer, Index 26 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT2520.1612572949728678No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.15997747517149585No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT2480.1586976553701239No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT2380.15229855636326406RNA PCR Primer, Index 26 (100% over 21bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA2310.14781918705846217RNA PCR Primer, Index 26 (100% over 29bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.12990170983925461No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1940.1241425207330808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAC851.4551915E-1163.089092
ATAGTAC300.00420610159.5841334
GATTACT954.0017767E-1156.4481283
CATGGGT709.0017784E-751.0721174
GGACCGT350.0077895250.99047
GTATCAA9900.050.68621
ATTACTT1101.72804E-1048.7506564
TATCAAC11800.042.9207762
TCAACGC13600.040.7450374
AACGCAG13650.040.5308276
ATCAACG12650.040.5077933
CAACGCA13750.040.236065
GTGTACT600.001296006339.7227554
TTAATAC650.001920405336.6671643
CTGTACC1001.0461388E-535.750484
CGCAGAG15600.035.0831388
GCAGAGT15900.034.4211929
TTAGGGC700.002762437334.0480773
ACGCAGA16200.033.7837647
ATACCGT750.003903838331.727366
Produced by FastQC (version 0.11.5)