FastQCFastQC Report
Fri 10 Feb 2017
SRR3558269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861513
Sequences flagged as poor quality0
Sequence length125
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13464915.629363689230457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT627357.28195627924361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488275.667587140298521No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252502.930890189701142No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161791.8779751437296943No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126241.465329019991573No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112351.3041010408432605No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83150.9651624525689108No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44900.521176116901312No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.4031279853002798No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.38095768723164947No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31870.3699305756268333No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.22855139736719005No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17780.20638109929855963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC17620.2045239015545906No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT17600.20429175183659443No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12520.1453257234655774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12150.141030953682649No Hit
GCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGA11970.13894160622068386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT11490.1333700129887767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11310.13128066552681156No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT9910.11503018526708245No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.11108364006114824No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT9150.10620849598322951No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA9140.10609242112423144No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9090.10551204682924112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT9070.10527989711124497No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA8720.10121727704631271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAC700.093.877771
TACAACG800.089.313912
TTGGACT1550.080.670624
TTAGGAC604.3655746E-1179.390143
ATCGACG401.9729596E-674.428262
CTTAGGA658.913048E-1173.283212
GTATCAA327100.072.908491
TTTGGAC1750.071.4511263
TGGACTG1750.071.393075
GGTATCA127450.071.294741
TAGGACC807.2759576E-1266.9854354
AGGACCG807.2759576E-1266.931015
TATCGAC453.8936996E-666.378221
ACGCGGA507.274404E-659.74041
ATGAACG507.4188574E-659.5426063
TCGACGC507.4188574E-659.5426063
TCACGCA603.1367017E-759.5426034
TACGTAA150.004235130759.506313114-115
CAAGGCA507.454699E-659.494235
TATCAAC417450.056.982322
Produced by FastQC (version 0.11.5)