FastQCFastQC Report
Fri 10 Feb 2017
SRR3558183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517324
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194081.2790939838821505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84210.5549902328045955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68770.45323213763177805No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39330.2592063395820537No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA28450.18750115334628595No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23050.15191218223662184No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA21450.14136730190783248No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19300.12719761896602177No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16550.10907360590091504No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15920.10492155927145422No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC15630.10301029971186114No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT15230.1003740796296638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5150.086.65751
GCATAGT8100.055.765432
TAGTACT8200.054.3605585
ATAGTAC8600.051.8321574
TACAACG3550.048.5518842
ACAACGC4000.044.5756573
GACCGTT2400.044.562437
CATAGTA10600.042.6132053
TAACGCA4950.042.0241854
GTATAAC5450.038.2141041
AGATTAC9700.037.376152
AACGCAG208050.037.244356
CATGGGC53750.035.7158134
CATGGGG50550.035.5076754
AGTGTAC9650.034.490323
ACATGGG206950.034.4054953
TACATGG215850.033.9781382
GTACATG214950.033.856431
GGGCTTT32400.033.375987
GTATCAA227050.032.6549221