FastQCFastQC Report
Fri 10 Feb 2017
SRR3558181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591519
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199621.2542734331164127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81730.5135345540958041No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54580.3429428112388228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50970.3202600785790179No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41520.26088284211498575No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.20364192950256954No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA27550.17310506503535303No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26210.16468543573780772No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25590.16078978636133154No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA23990.1507364976478446No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21360.13421140432505047No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21240.13345740767153896No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA18680.11737214572995988No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16180.10166388211513655No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC15930.10009305575365421No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5000.058.2883451
TACAACG3250.049.3627172
TAACGCA5050.048.240434
ATAGTAC7600.043.781754
GTATAAC6200.043.1694451
GCATAGT7750.042.934362
TAGTACT7750.041.400995
CATGGGG52250.039.460434
TAAGGTA5350.037.761035
GTACCCG5400.035.2007649
CATGGGT25700.034.911024
CATGGGC58750.034.7911874
ACAACGC4750.033.774493
ACATGGG254000.032.773523
ATAACGC8250.032.4098663
TACATGG281250.031.3516242
GTACATG281400.030.9648651
AACGCAG257700.030.5237336
AGATTAC7700.030.0948752
ATGGGTA10400.029.7090445
Produced by FastQC (version 0.11.5)