FastQCFastQC Report
Fri 10 Feb 2017
SRR3558176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5037126
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT672941.3359602281142064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244630.4856539224946924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244530.4854553965892456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130200.2584807288918323No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA103020.2045213877913715No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT92930.18449012393178174No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA76750.15236863243047724No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.11677293758385239No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC56030.11123406482188454No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC53670.10654885345333827No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA53600.10640988531952546No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51600.10243936721058794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10150.060.3637351
TAACGCA10650.038.499944
AACGCAG707100.036.5199936
GTATCAA720050.034.820891
TAGTACT18200.034.2829745
ACGCAGA796350.032.402427
CGCAGAG801150.032.2082868
ATAGTAC19050.032.1294174
CATGGGG154800.031.6312874
AGATTAC23300.031.6246932
GCATAGT19500.031.3879682
TATCAAC804300.031.1489282
ATCAACG810300.030.874283
GCAGAGT837700.030.788819
TCAACGC826150.030.7063244
GACCGTT10300.030.5724537
GATTACT24300.030.5678123
ACATGGG651700.030.5644723
CAACGCA839450.030.36145
TACAACG10600.029.7119122