FastQCFastQC Report
Fri 10 Feb 2017
SRR3558173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2696704
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325411.2066952843174483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127870.47417143297892544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122480.45418407062844124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66080.2450398708942472No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55450.20562138076704006No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48270.17899628583633947No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA39980.14825505505980635No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA30840.11436182836529332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6700.065.721541
ACAACGC6400.045.503313
TACAACG6450.044.2291342
ATAGTAC12050.038.4710924
TAGTACT12600.038.2068675
AGATTAC13800.037.468572
TAACGCA7650.035.737434
GATTACT15050.035.146373
AACGCAG371600.034.6521036
GCATAGT13200.034.218942
CATGGGT39900.033.663754
CATGGGG85450.032.828944
ACATGGG359200.032.8105353
GTATCAA383750.031.9735681
TACATGG383350.031.8133072
GTACATG384450.031.5903171
ATAACGC10500.031.131523
CATGGGC82500.031.121234
ACGCAGA416200.030.9044977
CGCAGAG419400.030.7536938