FastQCFastQC Report
Fri 10 Feb 2017
SRR3558171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3844682
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT519751.351867332590836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192760.5013678634539865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178810.46508397833682996No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101770.2647032966575649No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81250.21133087209813453No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74860.19471051181866275No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA64260.16713996112032153No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA48000.12484777674720562No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC47450.12341722930531056No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG44570.11592636270047821No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43010.11186880995619404No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA42420.11033422270034297No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.10781125721190986No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39120.10175093804897259No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38650.10052847023498952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9550.060.431611
TAACGCA8900.044.0703664
ACAACGC9400.037.9329073
ATAGTAC15500.037.5739754
ATAACGC12300.036.7198753
AACGCAG569950.036.1553846
TACAACG9000.035.6569332
TAGTACT16700.035.5857625
AGATTAC20000.035.359792
GCATAGT16950.035.0608982
GTATCAA593550.033.6102751
GTATAAC11150.033.083591
TCACGCA8000.032.685524
GATTACT22050.032.341893
ACGCAGA644650.031.8803657
CGCAGAG649150.031.705138
CATGGGG119300.030.5858554
GACCGTT7400.030.510457
GCAGAGT677400.030.3390589
TATCAAC660650.030.1166552