FastQCFastQC Report
Fri 10 Feb 2017
SRR3558169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3675961
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471581.2828754167957712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170060.46262732384810396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163550.4449176691482853No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA94040.25582425928893154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91570.24910492793585132No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90500.24619412447520525No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54400.1479885123917256No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG44720.12165526239260971No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA44380.12073033419016142No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38900.1058226678683479No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC37000.1006539514429016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7600.064.980741
ATAGTAC13050.038.2564134
TACAACG7150.037.4060552
TAGTACT14050.037.225615
GCATAGT13950.035.36222
AACGCAG522500.035.2129826
TAACGCA8300.035.0875784
GTATCAA527150.033.7599951
AGATTAC17300.032.9807552
CATGGGT60300.032.131854
CATGGGG118950.032.0279434
ACATGGG508150.031.930533
ACGCAGA587100.031.4159747
CGCAGAG589550.031.355978
TACATGG540700.031.1514972
GTACATG539200.030.9861371
GATTACT18450.030.9250413
CATGGGC121200.030.795874
TATCAAC590950.030.2023012
ACAACGC9100.030.0435923