FastQCFastQC Report
Fri 10 Feb 2017
SRR3558167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12611485
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2129711.6887067621299157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT851540.6752099376084577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT729650.5785599396106009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331840.26312523862178006No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA271740.21547026381112136No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT220790.17507058050657795No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA176250.13975356589648244No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC156400.12401394443239634No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC155420.12323687495961023No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150330.11920087126932315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC145920.11570405864178564No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT140020.11102578324439984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1233800.072.690731
GGTATCA481200.068.206021
TATCAAC1632150.055.434042
ATCAACG1721050.052.49123
TCAACGC1775500.050.9584164
AACGCAG1783350.050.7120176
CAACGCA1822400.049.6274265
ACGCAGA2095900.043.1032527
CGCAGAG2110900.042.7885178
GACCGTT30150.041.58327
GCAGAGT2196850.041.079299
GTACTGG111900.035.8920061
TACTGGT107950.034.575892
GGTTCAC112350.032.054536
AGAGTAC2178850.031.53168310-11
ACGGTAC38950.031.4337443
ATGGGAC184100.030.6371825
TACATGG1599750.030.6096782
GTACATG1588850.030.5958981
ACATGGG1609900.030.2099513
Produced by FastQC (version 0.11.5)