FastQCFastQC Report
Fri 10 Feb 2017
SRR3558161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3824713
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT625641.6357828678910027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228080.5963323261117893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227750.5954695162748159No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118410.30959185695763314No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94330.24663288461121133No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93250.2438091433265712No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA77890.20364926727835528No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA59890.15658691253435225No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA58910.15402462877606765No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53600.14014123412658674No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC50010.13075490893042171No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC49850.1303365768882528No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG47140.12325107792401678No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45340.11854484244961648No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA45330.11851869669698092No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT40440.10573342365819344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8250.062.7575151
AGATTAC15450.040.008672
AACGCAG644850.038.5051886
GACCGTT7200.037.1401867
TAACGCA7400.036.946684
TAGTACT14600.036.6386035
TACAACG7300.036.6386032
GCATAGT14700.036.3893622
GATTACT17250.036.1784063
GTATCAA671300.036.0809671
ATAGTAC15050.034.753254
ACGCAGA730500.034.0520367
CGCAGAG735000.033.9405758
GCAGAGT766950.032.5111669
TATCAAC749250.032.436922
CATGGGT54250.032.2104534
CATGGGG136800.032.1510284
ATCAACG759650.031.930253
TCAACGC775950.031.5199384
CAACGCA786600.031.1687415
Produced by FastQC (version 0.11.5)