FastQCFastQC Report
Fri 10 Feb 2017
SRR3558160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3545214
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501281.4139625985906634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195950.5527169869012138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180990.5105192521523383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99030.2793343363757449No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA85270.24052144666020162No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT76340.2153325582038207No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA59910.16898838828911317No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA47500.13398344923606867No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43220.12191083528385027No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39060.11017670583496511No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA39010.11003567062524294No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38980.10995104949940962No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.10098121016107912No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35750.10084017495135696No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT35730.10078376086746808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8650.063.9795721
TAACGCA7300.038.2641144
TAGTACT14500.037.298385
ATAGTAC15350.037.168884
GCATAGT15200.036.7536852
AACGCAG546150.034.9160776
AGATTAC17350.032.8842852
GTATCAA594950.031.5368251
TACAACG8350.031.3171942
CGCAGAG613450.031.1410088
ACGCAGA612950.031.1276367
GACCGTT7150.030.7486867
GATTACT18600.030.6743183
CATGGGG114150.030.6138554
GTATAAC9400.030.3870051
CATGGGC118300.030.1930074
ACATGGG500100.029.9950183
ATAACGC9600.029.7157463
GCAGAGT643500.029.6590949
TACATGG540100.028.9399782
Produced by FastQC (version 0.11.5)