FastQCFastQC Report
Fri 10 Feb 2017
SRR3558159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4382310
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT679341.550187001832367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263950.6023079152319211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223840.5107808438928327No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT136530.3115480191953559No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA133290.30415465815973763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117410.26791806147899166No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC83250.18996830438741213No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC81940.186979013351406No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA70150.16007539402735088No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70050.15984720387193058No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA69010.15747402625555929No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT65190.14875716231850325No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA61110.1394470039773544No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA59210.13511139102436842No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC56550.12904153289018805No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT55560.12678245035152694No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.11927499423819857No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG48850.1114708909228238No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA47400.10816213366922924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9500.057.6113171
GACCGTT10400.038.846687
ATAGTAC15250.037.407954
TACAACG6950.036.7659872
AACGCAG796950.036.5406726
TAGTACT16300.036.0919345
TAACGCA9550.036.0900274
GTATCAA790550.035.9475631
GCATAGT17250.033.7597772
ACGGTAC15000.032.8813323
ACGCAGA885750.032.8337067
CGCAGAG889200.032.7597668
TTAGGAC13450.032.69433
TATCAAC878550.032.4463462
ATCAACG888100.032.010463
TCAACGC906200.031.7448734
GCAGAGT926000.031.4450349
CAACGCA917100.031.3934945
GTATAAC12400.030.7044871
GTACTGG39400.030.3489321
Produced by FastQC (version 0.11.5)