FastQCFastQC Report
Fri 10 Feb 2017
SRR3558153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4411747
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494661.1212338332184506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201030.45566982875491274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156240.3541454213036242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83840.1900380960195587No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA53180.12054181710782598No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA47110.10678309522282217No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46770.10601242546320086No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA46040.10435775215577865No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45960.10417641809469128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA450300.046.7902371
AACGCAG491200.043.835816
TATCAAC524150.040.2900732
ATCAACG528450.039.8272633
TCAACGC540200.039.4010854
CAACGCA549100.038.7841075
ACGCAGA561250.038.3192337
CGCAGAG568300.037.9066128
GCAGAGT602600.035.7489669
CGCATAG7800.031.2766721
GTACATG495300.030.85021
ACATGGG491450.030.7919713
TACATGG501450.030.7587152
CATGGGG162350.029.7277584
GGTATCA268250.029.634531
CATGGGC134250.028.2022364
TAACGCA8700.027.3274564
ATGGGAC49900.027.0385725
AGAGTAC594150.026.97049510-11
GAGTACT292950.026.18890612-13