FastQCFastQC Report
Fri 10 Feb 2017
SRR3558152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5304815
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660621.2453214673838766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241930.4560573742910921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241660.45554840272469443No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA174300.3285694223078467No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT167570.31588283474541523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126570.23859455984798716No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG72360.13640437979458284No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70900.1336521631762842No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT70210.13235145806215673No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG66940.12618724686911797No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC62320.1174781778440907No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC61500.11593241234614214No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT60180.11344410691042006No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59660.11246386537513561No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53990.10177546248078397No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53290.10045590656790106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9150.047.4771841
TAACGCA10550.035.4903264
AACGCAG786150.033.0626686
GTATCAA788950.032.0117261
GACCGTT10650.031.2421997
CATGGGG172300.029.974814
ACGCAGA876800.029.6266697
CGCAGAG882550.029.453848
TATCAAC872300.029.0041182
ATCAACG874600.028.8870723
TCAACGC887300.028.821914
CAACGCA897050.028.6411535
CATGGGT59800.028.225334
GCAGAGT920500.028.2137099
ACATGGG731200.028.0579853
ATAACGC14250.027.5264993
TACTGGT32550.027.2055282
TACATGG802000.026.8186022
GTACATG804800.026.6489471
GTACTGG37100.026.6267031
Produced by FastQC (version 0.11.5)