FastQCFastQC Report
Fri 10 Feb 2017
SRR3558150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481543
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT498021.430457702231453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183900.5282140706003057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181390.5210046235246844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99780.28659706342848557No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA96550.27731956778933936No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT91340.26235493860049985No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56560.16245670382356328No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA53210.1528345334238296No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG50000.14361448357811465No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA44350.1273860469337877No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42270.12141168441693811No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37650.10814170613432032No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37610.10802681454745784No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA37030.10636088653795171No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36780.10564281412006114No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35600.10225351230761762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9200.064.018191
TAGTACT13750.043.2169345
TACAACG6450.042.3794022
ATAGTAC14350.040.5817534
GCATAGT15100.038.5661052
CATGGGT49850.036.5956844
AGATTAC16150.036.0587122
AACGCAG581450.034.456866
TATCACG6300.033.956162
CATGGGG119150.033.713924
GTATCAA609650.031.7926351
ATGGGTG31800.030.6459715
ACGCAGA654200.030.594787
CGCAGAG657900.030.5130188
ACATGGG552350.030.3921033
CATGGGC132900.029.6445714
TACATGG594900.029.4769062
TAACGCA9700.029.4053364
GATTACT20050.029.3411733
GTACATG594750.029.29821