FastQCFastQC Report
Fri 10 Feb 2017
SRR3558148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5575826
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1165122.0895917483795228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470050.8430141112724823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390680.7006674885478851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203010.364089553727107No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA114990.20622953442234387No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT111430.1998448301650733No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC99440.17834128970308616No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA92420.1657512268137492No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC88750.15916924236875396No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84790.15206715561066647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC72920.1307788298989244No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA71590.12839353308370813No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA68750.12330011732790802No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT66290.11888821494788396No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65020.11661052550779023No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA62410.11192960468995984No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT61440.11018995212547882No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59450.10662097418391464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT16600.060.4917837
GTATCAA887900.052.7865221
AACGCAG1045150.045.172686
TTAGGAC23200.045.0858043
TATCAAC1046900.044.9722672
ATCAACG1078950.043.58683
TCAACGC1107750.042.5716324
CAACGCA1127950.041.8408475
GGACCGT29200.039.8872226
CGCAGAG1216050.038.7764478
ACGCAGA1216800.038.747667
TAGGACC29900.038.3624
GGTATCA489150.037.9961971
GCAGAGT1258850.037.448639
CTTAGGA29500.036.0616952
AGGACCG31450.035.7154665
TACTGGT47200.035.1299362
GTACTGG50400.034.5866281
TCGATCA17150.034.53692242-43
ACGGTAC19550.033.4395983
Produced by FastQC (version 0.11.5)