FastQCFastQC Report
Fri 10 Feb 2017
SRR3558145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105856
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327551.0546206907210123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126520.40735951698984113No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93750.30184915205341134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80820.2602181170022049No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75960.244570256959756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46850.15084408291949145No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC46580.14997475736157762No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46470.14962058768983494No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40350.12991587504378826No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40250.12959390261493128No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35730.11504074883059615No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA35510.11433240948711079No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT33400.10753879123822868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC5550.037.4774743
TACAACG5700.034.4060132
TAACGCA7050.032.8753784
CATGGGG92600.032.088864
CGCATAG4850.031.8878781
AACGCAG423750.031.4252266
CATGGGT44000.031.1999974
GTATCAA437550.029.4730851
ACATGGG392900.028.9806923
GTACTGG22050.028.8648431
ACGCAGA463450.028.7186157
CGCAGAG466050.028.6603958
TACTGGT19300.028.6365622
GCAGAGT487850.027.4040379
ATCAACG471150.027.3082563
TCAACGC477600.027.3003084
TATCAAC473250.027.3000982
CAACGCA481750.027.188495
AGATTAC12800.026.928572
TACATGG450350.026.8804242