FastQCFastQC Report
Fri 10 Feb 2017
SRR3558144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087999
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398251.2896701067584542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149380.4837436799688083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147990.47924238317434686No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA106600.3452073656759604No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT99830.32328378344682107No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82020.2656088943033984No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA52310.16939772325055805No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG49530.16039512966163524No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44520.14417103114346863No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44510.14413864771329266No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40930.13254537971029134No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT39940.12933942012286923No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA38410.12438475530594406No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.11107516550361578No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32830.10631480126774653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7900.061.7521671
TACAACG6200.045.051282
AGATTAC15200.044.9629942
TAGTACT12350.040.902795
TATCACG7200.040.4449732
GATTACT17100.039.9671063
ATAGTAC12950.037.630944
GCATAGT13850.036.4728852
TCACGCA8250.036.0177884
CATGGGT49150.035.1860434
AGTGTAC17750.032.8116953
ATCACGC9500.031.9041753
CATGGGG98450.031.7519934
AACGCAG501600.030.9433566
ATTACTT22400.030.5106014
TAACGCA7700.030.100584
ACAACGC9100.030.041213
ACATGGG467400.028.9772113
ATAACGC12150.028.8586983
CATGGGC99000.028.6341424