FastQCFastQC Report
Fri 10 Feb 2017
SRR3558143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305794
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAA218290.9467020904729564No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCA194020.8414455064069036No Hit
GCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGT183570.7961248923364359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131560.5705626781924145No Hit
GACTGGGAGCGTTCCTTCGGGGACTTTCCCCGGGCGTCGAACAGCCGCCT128550.5575086065797725No Hit
CTCCCAGTCCGTCCCCCGGCCGGCACGCGGCAACCCGCTCTCGCCGCGGG128310.5564677503714556No Hit
CCCCTTGTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGT120960.5245915289917487No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT111010.4814393653552746No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTTGCCGCGTG107190.4648724040395629No Hit
GTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGTCCCCGAAGGAACGCTC107170.4647856660222032No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA96590.4189012548388971No Hit
GTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGTCCCCGA94640.4104442981463219No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA85580.3711519762823566No Hit
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCG82600.3582280116957543No Hit
CCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAA79940.34669185538690794No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACC77360.3355026511475006No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAG75530.3275661225590838No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGA68040.2950827350578586No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC66790.2896616089728744No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGC65550.28428385189657013No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG62540.27122978028392825No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA60040.2603875281139599No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAA55040.23870302377402317No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAA54770.2375320605396666No Hit
GTACTGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAAT54750.23744532252230685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52940.22959553195124976No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGAT52900.22942205591653025No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCC51960.22534536910062217No Hit
GAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGC50060.2171052574514462No Hit
GGGGACGGACTGGGAGCGTTCCTTCGGGGACTTTCCCCGGGCGTCGAACA47560.20626300528147787No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCG46980.20374760277804524No Hit
TCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTTGCCGCGTGC46580.20201284243085027No Hit
TACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGTCCCCGAAGGAA46090.19988776100553649No Hit
GCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCG45840.19880353578853965No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCC44270.19199460142579952No Hit
GGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGG41550.18019823106487398No Hit
GCTCGAGCTGCTCCCGCGGCGAGAGCGGGTTGCCGCGTGCCGGCCGGGGG41310.179157374856557No Hit
ACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTT40250.17456025993649044No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT40200.17434341489309108No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACC39440.17104737023342068No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGG38490.16692731440883268No Hit
GGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCG38270.16597319621787548No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG37740.1636746387578422No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCC37220.1614194503064888No Hit
GAGTACGACCGGGCGTGAATGGCACTCGGTCCTCCGGATTTTCAAGGGTC36530.15842698870757752No Hit
GTCCGTCCCCCGGCCGGCACGCGGCAACCCGCTCTCGCCGCGGGAGCAGC36080.15647538331698324No Hit
GGTTAAAATTCCTGAACCGGGACGTGGTGGTTGACGGCAACGTTAGGGAG33990.1474112605028897No Hit
GAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTTCCCTTG33180.14389837079981993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33180.14389837079981993No Hit
CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAAC32160.13947473191447285No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT31640.13721954346311943No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGG31120.134964355011766No Hit
GCATTGCGATGGTCCCTGCGGATGCTAACGCAATGTGATTTCTGCCCATG31050.1346607719510069No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGGCGG31010.1344872959162874No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCA30670.1330127496211717No Hit
CCCTAACGTTGCCGTCAACCACCACGTCCCGGTTCAGGAATTTTAACCCG29900.12967333595282146No Hit
TCCCAGTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCG29750.12902280082262335No Hit
CCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCAT29180.12655076732787057No Hit
CCTTCGGGGACTTTCCCCGGGCGTCGAACAGCCGCCTCAGAACTGGTACG28470.12347156771159956No Hit
TCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAG28340.12290777059876119No Hit
CCCCGAAGGAACGCTCCCAGTCCGTCCCCCGGCCGGCACGCGGCAACCCG28200.12230060447724297No Hit
CCGGAGGACCGAGTGCCATTCACGCCCGGTCGTACTCATAACCGCATCAG27770.12043573710400841No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGGCGGCTGT27110.11757338253113678No Hit
GCTCCCGCGGCGAGAGCGGGTTGCCGCGTGCCGGCCGGGGGACGGACTGG26880.11657589533149969No Hit
GCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTT26850.11644578830546007No Hit
GTTCGACGCCCGGGGAAAGTCCCCGAAGGAACGCTCCCAGTCCGTCCCCC26270.11393038580202741No Hit
GGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGC26180.11354006472390857No Hit
CCCGGGGAAAGTCCCCGAAGGAACGCTCCCAGTCCGTCCCCCGGCCGGCA26150.11340995769786893No Hit
GGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGGACT25690.11141498329859476No Hit
CCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTTC24590.1066443923438087No Hit
GGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACC24360.10564690514417159No Hit
GATTCCCCTTGTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGA23570.1022207534584616No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG23280.10096305220674527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGG12900.067.320851
ACAACGC3150.064.140263
TACAACG3200.063.1380652
CGCATAG2850.060.5256651
ACTGGGC14250.055.462463
TACTGGG16100.053.5185662
GTATCAA182000.053.109031
TAATCCG2200.051.3207785
ATAGTAC4200.049.520054
GTATAAC3100.047.969451
TATCAAC217150.045.070892
ATCAACG223400.043.7833483
AACGCAG230700.043.604776
TCAACGC231150.042.726714
GGTATCA99000.042.2983931
CAACGCA235200.042.1173065
GTACAAC5100.041.9873771
GGTTTCA5750.041.378871
CATGGGG90000.041.2006844
TGGGCGA3750.039.6126026
Produced by FastQC (version 0.11.5)