FastQCFastQC Report
Fri 10 Feb 2017
SRR3558141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5016342
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT886291.766805373317848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335430.6686745042503083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307410.6128170686926848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159000.31696403474882695No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA102300.20393346386669808No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT78330.15614964051494096No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77820.1551329634223504No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA75510.15052801423826367No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71370.14227498842782252No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA63890.1273637244031607No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60530.12066561649903455No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA55860.11135604390609731No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC55390.11041910619331775No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC52830.10531578588541211No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA51150.10196673193334904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9500.051.35821
AACGCAG797500.041.6136866
GTATCAA824150.039.3468321
ACGCAGA908600.036.5224157
CGCAGAG912150.036.3281488
TATCAAC924550.035.195582
ATCAACG928450.034.9517173
GCAGAGT957800.034.5594759
TCAACGC949800.034.5540244
CAACGCA964950.034.0977445
GACCGTT10650.030.690217
ACATGGG727650.030.0416373
TACAACG9400.029.716962
CATGGGG168400.029.5757884
TACATGG758100.029.5092052
GTACATG755800.029.3959141
ATAGTAC16800.029.0094154
TAGTACT18200.028.4107235
CATGGGC188800.028.237414
CATGGGT56600.027.721834
Produced by FastQC (version 0.11.5)