FastQCFastQC Report
Fri 10 Feb 2017
SRR3558133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334112
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416251.7833334475809215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160260.6865994433857502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144840.6205357754897795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72480.31052494481841486No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59310.25410091717963834No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52230.22376818250366737No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA52040.2229541684375043No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA41610.17826908048971085No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA35340.1514066163063298No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34380.14729370312992693No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31240.1338410496154426No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.12073113886565855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA402300.039.0953371
AACGCAG411000.038.8312646
CGCATAG4550.035.2992861
TATCAAC452950.034.7388532
ATCAACG458150.034.357253
ACGCAGA466850.034.185827
CGCAGAG468400.034.0600178
TAACGCA4550.033.942374
TCAACGC468000.033.9387444
CAACGCA475650.033.442855
GCAGAGT489100.032.630659
GGACCGT7150.031.5552546
AGATTAC8050.031.0028172
GTATAAC6600.030.6442181
GACCGTT4750.029.9993427
ACATGGG376250.029.9324343
TACATGG393750.029.4582562
GTACATG392100.029.4167371
ATGGGAC33050.028.9357415
CATGGGG85450.028.778524