FastQCFastQC Report
Fri 10 Feb 2017
SRR3558117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026907
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388021.2819026154420998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147460.4871639597780837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141600.4678042635601292No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132410.4374432382626886No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125520.4146807285456739No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76820.2537904203862226No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC60690.2005017002504537No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA58310.19263888847592608No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55480.18328941060957604No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48620.16062601196534945No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG47790.1578839389515436No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT45580.15058275658948228No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC39610.13085965310463782No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC38030.12563980327112792No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC36900.12190661952944046No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA36780.12151017523828779No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT34770.11486973336148087No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33170.10958380947944553No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32740.10816321743614853No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA32020.1057845516892326No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31740.10485951500987642No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA30630.10119240531671439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7400.057.851561
TAGTACT11550.039.5892645
TAACGCA7000.039.02374
ATAGTAC11450.038.8977474
GCATAGT12650.034.744152
CATGGGT39650.033.9978454
AGATTAC14400.033.408962
GTATAAC9050.032.8500251
ATAACGC9650.031.3842353
GATTACT15150.030.9658583
TACAACG6350.030.866022
CATGGGG93950.030.592664
AACGCAG546000.029.504136
TACTGGT19950.029.4735682
GTACTGG23050.029.1488761
AGTGTAC15750.029.0321273
ACAACGC6900.028.4009933
TTCAACG17600.028.0049084
ACATGGG474900.027.9350643
CATGGGC98850.027.754544
Produced by FastQC (version 0.11.5)