FastQCFastQC Report
Fri 10 Feb 2017
SRR3558111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5197848
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569191.0950493358020472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210010.4040325919495914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195540.3761941480397272No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA156240.3005859347945534No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT150480.2895044256777035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103630.1993709704477699No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG73880.14213574540848445No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC65730.12645617955738606No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG63270.12172345170539808No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA62150.11956871382156616No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT57120.10989163207542814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11400.043.2882731
AACGCAG744750.031.5707386
GTATCAA766050.029.7416731
TAGTACT18750.029.4499725
CATGGGG161950.029.1833634
ACGCAGA821750.028.533027
CGCAGAG830150.028.3158118
TATCACG9450.028.280042
ATAGTAC18700.027.3061244
GCAGAGT869550.027.053289
TATCAAC845900.026.9805432
TCAACGC858850.026.8858534
ATCAACG847750.026.8806883
CAACGCA865750.026.7401565
ACATGGG669050.026.08223
TACAACG8700.025.9396232
CATGGGT57150.025.1421454
AGTACTT377500.024.72658312-13
TACATGG760400.024.56282
GAGTACT339550.024.41336312-13
Produced by FastQC (version 0.11.5)