FastQCFastQC Report
Fri 10 Feb 2017
SRR3558107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4661886
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556211.1931008179951206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213470.457904805051003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202850.4351243252194498No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA181880.3901425302978237No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT165520.3550494370733218No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112370.2410397851856523No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC77210.16561966551734641No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG75280.16147971014306226No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA70340.15088314042857334No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT70310.15081878879063107No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70210.15060428333082362No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC59130.12683707838415612No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC54010.11585439884201373No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC53230.11418125625551546No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT52610.11285132240470917No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA51840.11119963036419166No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC49820.10686662007608079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8450.054.1843831
ACGGTAC12150.033.2295723
CATGGGT66500.032.052724
CATGGGG160600.031.7570194
ATAGTAC15150.030.96044
TAACGCA8550.030.5547454
TCACGCA8800.030.361414
CACGGTA13500.029.9133552
AACGCAG774850.029.8801776
TAGTACT15500.029.8782395
GTACTGG36250.029.5261
GTATCAA779250.029.1034181
TACAACG9200.029.047892
ACATGGG708650.027.900023
ACGCAGA857800.026.9699767
TATCACG9250.026.9648172
CGCAGAG860500.026.9474438
ATGGGAC50700.026.8175815
CATGGGC144600.026.4839974
TATCAAC859750.026.3657362
Produced by FastQC (version 0.11.5)