FastQCFastQC Report
Fri 10 Feb 2017
SRR3558105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4377640
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503221.1495234875412323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185420.4235615537138733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180360.4120028143017699No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA178400.407525516031469No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT167280.38212370135506807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92830.21205489715919995No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC84460.19293500607633338No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA78030.1782467265467238No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG72410.16540875905739166No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG67060.15318756224815197No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT63540.14514670004842792No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA57370.13105234784038888No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50450.11524474374320409No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC49730.11360002192962418No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC49180.1123436372109173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9150.057.8429681
TAACGCA10800.047.8367274
ATAACGC15050.034.722593
TACAACG9450.033.9431342
GTATAAC15350.032.542621
CATGGGG126200.032.2797934
ATAGTAC17300.031.236424
TAGTACT17450.030.9675585
GCATAGT18300.029.2133562
CATGGGT46500.029.1170834
AGTGTAC19250.028.997673
AGATTAC18950.028.2113112
ACATGGG611100.027.7239723
AACGCAG703450.027.4378766
GACCGTT8700.027.2965347
ACAACGC11700.026.9002273
TATCACG8000.026.7302192
TACATGG717900.025.997542
CATGGGC120500.025.971054
GATTACT20600.025.6559873
Produced by FastQC (version 0.11.5)