##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3558103.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2743242 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.272248675107775 33.0 30.0 33.0 18.0 34.0 2 31.387864796470744 33.0 32.0 33.0 27.0 34.0 3 31.738018373880248 33.0 32.0 34.0 27.0 34.0 4 31.658544160522478 33.0 32.0 34.0 27.0 34.0 5 31.810466958438226 33.0 32.0 34.0 28.0 34.0 6 35.06585201013983 37.0 35.0 38.0 29.0 38.0 7 35.387010333029316 38.0 36.0 38.0 29.0 38.0 8 35.589051202919755 38.0 36.0 38.0 29.0 38.0 9 35.666702026288604 38.0 36.0 38.0 29.0 38.0 10-11 35.676362129188746 38.0 36.0 38.0 29.0 38.0 12-13 35.623394144592424 38.0 36.0 38.0 29.0 38.0 14-15 35.62848884640874 38.0 36.0 38.0 29.0 38.0 16-17 35.61043830620849 38.0 36.0 38.0 29.0 38.0 18-19 35.53874266287845 38.0 36.0 38.0 29.0 38.0 20-21 35.5094244328426 38.0 36.0 38.0 29.0 38.0 22-23 35.4064080019189 38.0 36.0 38.0 29.0 38.0 24-25 35.31689548351913 38.0 36.0 38.0 28.5 38.0 26-27 35.05122606755073 38.0 36.0 38.0 27.0 38.0 28-29 35.03041711230726 38.0 36.0 38.0 27.0 38.0 30-31 34.92189733898796 38.0 35.5 38.0 27.0 38.0 32-33 34.84240453448875 38.0 35.5 38.0 26.0 38.0 34-35 34.59067245981215 38.0 35.0 38.0 25.0 38.0 36-37 34.40241965528378 38.0 35.0 38.0 24.0 38.0 38-39 34.18377343304017 38.0 34.0 38.0 16.0 38.0 40-41 33.892577104025094 38.0 34.0 38.0 16.0 38.0 42-43 33.37455262787607 38.0 33.5 38.0 15.5 38.0 44-45 32.98505873707096 38.0 32.5 38.0 15.0 38.0 46-47 32.40607573083235 38.0 29.5 38.0 15.0 38.0 48-49 31.868010004221283 38.0 27.0 38.0 15.0 38.0 50-51 31.43531522191626 38.0 25.5 38.0 15.0 38.0 52-53 31.106448319178547 37.0 25.0 38.0 15.0 38.0 54-55 30.87959192080028 37.0 24.0 38.0 15.0 38.0 56-57 30.619927625780008 37.0 24.0 38.0 15.0 38.0 58-59 30.48089632631755 37.0 20.0 38.0 15.0 38.0 60-61 30.333054283945785 37.0 16.0 38.0 15.0 38.0 62-63 30.204498363615023 37.0 16.0 38.0 15.0 38.0 64-65 29.993960248494297 37.0 16.0 38.0 15.0 38.0 66-67 29.867136402840142 36.0 15.5 38.0 15.0 38.0 68-69 29.69311730426991 36.0 15.0 38.0 14.5 38.0 70-71 29.542244176780613 36.0 15.0 38.0 14.0 38.0 72-73 29.322658190564304 36.0 15.0 38.0 14.0 38.0 74-75 29.181551427107053 36.0 15.0 38.0 14.0 38.0 76-77 29.06067073192959 36.0 15.0 38.0 14.0 38.0 78-79 28.88954200905352 36.0 14.0 38.0 14.0 38.0 80-81 28.70381668842924 35.0 14.5 38.0 14.0 38.0 82-83 28.56243689036549 35.0 14.0 38.0 13.5 38.0 84-85 28.46368384561041 35.0 14.0 38.0 13.0 38.0 86-87 28.41712123830125 35.0 14.0 38.0 13.0 38.0 88-89 28.304258063998727 34.5 14.0 38.0 13.0 38.0 90-91 28.17636449864795 34.0 14.0 38.0 13.0 38.0 92-93 28.09896155716484 34.0 14.0 38.0 13.0 38.0 94-95 28.044083059387397 34.0 14.0 38.0 13.0 38.0 96-97 27.97487607728374 34.0 14.0 38.0 13.0 38.0 98-99 27.870676374887815 34.0 14.0 38.0 13.0 38.0 100-101 27.765695480019627 34.0 14.0 38.0 13.0 38.0 102-103 27.612293410497507 34.0 14.0 38.0 13.0 38.0 104-105 27.527932278668814 34.0 14.0 38.0 13.0 38.0 106-107 27.32879563669556 33.0 14.0 38.0 13.0 38.0 108-109 27.17126870323508 33.0 14.0 38.0 13.0 38.0 110-111 26.9749518999782 31.0 14.0 38.0 13.0 38.0 112-113 26.79611641991483 31.0 14.0 38.0 13.0 38.0 114-115 26.662013777858462 31.0 14.0 38.0 13.0 38.0 116-117 26.501959178227807 29.0 14.0 38.0 13.0 38.0 118-119 26.39650566738188 28.5 14.0 38.0 13.0 38.0 120-121 26.192371835951768 28.0 14.0 38.0 13.0 38.0 122-123 26.128244427578757 27.5 14.0 38.0 13.0 38.0 124-125 25.06217643211937 25.0 14.0 36.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.44305427539879005 1101 2 -0.15637089151712047 1101 3 -0.08761986162319602 1101 4 -0.05702540014364743 1101 5 -0.3232689477413473 1101 6 0.3674440841626918 1101 7 0.3947953974676537 1101 8 0.25076892741000734 1101 9 0.22144863666110126 1101 10-11 0.2877889903086057 1101 12-13 0.3256984268933465 1101 14-15 0.21362968402515747 1101 16-17 0.21954055212530932 1101 18-19 0.07425017614681195 1101 20-21 0.31569875494667343 1101 22-23 0.171496503975213 1101 24-25 -0.013280973381448291 1101 26-27 0.21261744859187104 1101 28-29 0.2366467950452389 1101 30-31 0.09666610766158357 1101 32-33 0.2312896624285372 1101 34-35 0.34639322637664094 1101 36-37 0.32329714779681495 1101 38-39 0.2794843604341608 1101 40-41 0.30430896959905596 1101 42-43 0.3744990070988763 1101 44-45 0.25894613594608273 1101 46-47 0.24285865742147905 1101 48-49 0.16272493666299326 1101 50-51 0.026873392187550138 1101 52-53 0.05539794197144943 1101 54-55 0.12411123457234297 1101 56-57 0.05856371818812178 1101 58-59 -0.1386827683391978 1101 60-61 -0.09404055653022425 1101 62-63 0.034898193035292735 1101 64-65 -0.06263656594335387 1101 66-67 -0.058968182405660485 1101 68-69 -0.031518496428400766 1101 70-71 -0.027279270487543528 1101 72-73 -0.05480051178745882 1101 74-75 -0.32669804725939144 1101 76-77 -0.2684911354688886 1101 78-79 -0.1709660880968933 1101 80-81 0.02637964668311099 1101 82-83 -0.08374287251748314 1101 84-85 -0.11466170943266363 1101 86-87 0.12199487947616561 1101 88-89 -0.09327408216470445 1101 90-91 0.007218576471110794 1101 92-93 -0.06299727936332289 1101 94-95 -0.1739327661033343 1101 96-97 -0.07455136745796764 1101 98-99 -0.18941147298162875 1101 100-101 -0.19818887386532325 1101 102-103 0.015588557649543588 1101 104-105 -0.041694984879008246 1101 106-107 -0.21708412893258 1101 108-109 -0.37528270376200723 1101 110-111 -0.4004819930486079 1101 112-113 -0.2668836069934777 1101 114-115 -0.25932060692214876 1101 116-117 -0.20891423733114678 1101 118-119 -0.24419167229977745 1101 120-121 -0.5025714966780477 1101 122-123 -0.29531613040196447 1101 124-125 -0.3768671501277687 1102 1 -0.2387726775044392 1102 2 -0.47106865809671916 1102 3 -0.2464038583563557 1102 4 -0.1996136197922027 1102 5 -0.17475636622791768 1102 6 0.18051176753129283 1102 7 0.31835204647435944 1102 8 0.40031673350265606 1102 9 0.29265283174309076 1102 10-11 0.2210615606392352 1102 12-13 0.33961061671219994 1102 14-15 0.3714436520918909 1102 16-17 0.23058523412868936 1102 18-19 0.1925172922677163 1102 20-21 0.14804885563882664 1102 22-23 0.24304812220574945 1102 24-25 0.14308051626420593 1102 26-27 0.26416417990981245 1102 28-29 0.0788614390236475 1102 30-31 0.09953893002540326 1102 32-33 -0.038864588011271906 1102 34-35 -0.03955786395857075 1102 36-37 -0.012071369164871726 1102 38-39 0.03223136395585868 1102 40-41 0.06846772974211746 1102 42-43 0.00102196508255048 1102 44-45 -0.015295620138886079 1102 46-47 0.06602052200233999 1102 48-49 -0.10813837340085897 1102 50-51 -0.12982853753731405 1102 52-53 -0.21197673788880778 1102 54-55 -0.2096781199671085 1102 56-57 -0.18049473912456193 1102 58-59 -0.14348705824262353 1102 60-61 -0.23624223548256396 1102 62-63 -0.1493409453256369 1102 64-65 -0.1638365044429051 1102 66-67 -0.1474252553155786 1102 68-69 -0.25984675704594196 1102 70-71 -0.17799392892646537 1102 72-73 -0.29725119586870363 1102 74-75 -0.373186811686228 1102 76-77 -0.3749527858116508 1102 78-79 -0.3504747222604401 1102 80-81 -0.3108336983208062 1102 82-83 -0.44103025535950025 1102 84-85 -0.3911330421409396 1102 86-87 -0.35427399365913814 1102 88-89 -0.4273314506129502 1102 90-91 -0.31535850240098995 1102 92-93 -0.24010756909519415 1102 94-95 -0.2784960103409446 1102 96-97 -0.2714206535460413 1102 98-99 -0.25167332250871866 1102 100-101 -0.32360195640470835 1102 102-103 -0.3738975724916358 1102 104-105 -0.20126998415598507 1102 106-107 -0.5381357470395223 1102 108-109 -0.39807797574205495 1102 110-111 -0.6240809236131675 1102 112-113 -0.3198479013858382 1102 114-115 -0.32662386172458113 1102 116-117 -0.5456766490880156 1102 118-119 -0.701941198378023 1102 120-121 -0.8407839767701191 1102 122-123 -1.0364630816186136 1102 124-125 -0.7253934382321567 1103 1 -0.10027645397440565 1103 2 0.011117315651105741 1103 3 -0.08116662565207378 1103 4 -0.09766272806214005 1103 5 -0.11084805688052768 1103 6 0.21186350002606957 1103 7 0.2009946386845911 1103 8 0.16948804022202069 1103 9 0.18651385019300193 1103 10-11 0.11414127594982659 1103 12-13 0.18773081745585785 1103 14-15 0.13991994939640762 1103 16-17 0.1308097793559142 1103 18-19 0.10855773437237559 1103 20-21 0.05648939853649182 1103 22-23 0.05332864579686003 1103 24-25 0.006017337397047129 1103 26-27 0.09652579103906334 1103 28-29 0.03840662631491654 1103 30-31 0.06089265465861615 1103 32-33 0.13380255857598655 1103 34-35 0.10722322278078167 1103 36-37 0.1715939912754223 1103 38-39 0.21141943562152932 1103 40-41 0.18429339366522157 1103 42-43 0.028638377495390444 1103 44-45 0.11811986369054495 1103 46-47 -0.07029971993960515 1103 48-49 -0.2336987077218744 1103 50-51 -0.16362517820612865 1103 52-53 -0.04439977126935091 1103 54-55 -0.14732116119517613 1103 56-57 -0.07003774305146848 1103 58-59 -0.14172336216984505 1103 60-61 -0.03663050883106678 1103 62-63 -0.13817183843058345 1103 64-65 -0.09734537430010803 1103 66-67 -0.07133246571904195 1103 68-69 -0.2935094790525845 1103 70-71 -0.22182363715685227 1103 72-73 -0.18966277021722178 1103 74-75 -0.25378201569161263 1103 76-77 -0.3226764590244571 1103 78-79 -0.18054422441221618 1103 80-81 -0.4003898217985338 1103 82-83 -0.19894894512787076 1103 84-85 -0.28233154215510226 1103 86-87 -0.15451570080800892 1103 88-89 -0.18809783328894625 1103 90-91 -0.21513838683759445 1103 92-93 -0.27603867838226037 1103 94-95 -0.30096820330774676 1103 96-97 -0.36478344374046046 1103 98-99 -0.2640833404234435 1103 100-101 -0.2074171992410605 1103 102-103 -0.31661203418218165 1103 104-105 -0.08646253777004631 1103 106-107 -0.3273232038775298 1103 108-109 -0.33014175854425787 1103 110-111 -0.6742786366963394 1103 112-113 -0.604077160453155 1103 114-115 -0.5465935245787499 1103 116-117 -0.5506583264976257 1103 118-119 -0.4698330463200051 1103 120-121 -0.5237569904350288 1103 122-123 -0.49605250503372034 1103 124-125 -0.7640366273577435 1104 1 -0.30400935223580916 1104 2 -0.11751632789134447 1104 3 -0.28441750545676214 1104 4 -0.06347459801750688 1104 5 -0.18027252707932107 1104 6 -0.01296298867665513 1104 7 0.1281030119696993 1104 8 -0.052020159286165324 1104 9 -0.013191012373297895 1104 10-11 -0.027543994213083067 1104 12-13 -0.062996519235746 1104 14-15 0.07322644489909891 1104 16-17 0.11143164573421416 1104 18-19 0.002757752552845716 1104 20-21 0.04686172031185265 1104 22-23 0.08332127916651189 1104 24-25 0.02894798128067322 1104 26-27 0.07649983075039302 1104 28-29 0.15201015993638833 1104 30-31 0.09874981522400361 1104 32-33 -0.02065679096472195 1104 34-35 -0.04513468920450947 1104 36-37 -0.011778555984939487 1104 38-39 0.099832517057024 1104 40-41 0.1296358298240463 1104 42-43 0.0647866923196645 1104 44-45 -0.030486438320046716 1104 46-47 0.07210356057199618 1104 48-49 -0.07952770745388804 1104 50-51 -0.06719887933579827 1104 52-53 -0.14906453646391427 1104 54-55 -0.06453151036637195 1104 56-57 -0.08040326486035454 1104 58-59 -0.17969952214849982 1104 60-61 -0.11839054320867604 1104 62-63 -0.0563543703083802 1104 64-65 -0.01940831412866828 1104 66-67 0.07744666877272621 1104 68-69 -0.05594214344708348 1104 70-71 -0.23975565638092533 1104 72-73 -0.08138351589965609 1104 74-75 -0.17123886364743157 1104 76-77 -0.2564253065127815 1104 78-79 -0.27197774234417693 1104 80-81 -0.07075495402600751 1104 82-83 0.030659773468027396 1104 84-85 -0.00262595945346078 1104 86-87 -0.09307233709126095 1104 88-89 -0.1481199922074694 1104 90-91 -0.3575343479408417 1104 92-93 -0.0888213167456442 1104 94-95 -0.26941163667693147 1104 96-97 -0.17667612468626714 1104 98-99 -0.17295959874189393 1104 100-101 -0.16260713198514054 1104 102-103 -0.14097173351977688 1104 104-105 -0.08967209833401668 1104 106-107 -0.20886123587751015 1104 108-109 -0.33980349562875034 1104 110-111 -0.32863935130670185 1104 112-113 -0.4716152892582137 1104 114-115 -0.26783934690837796 1104 116-117 -0.2894758642722657 1104 118-119 -0.36389924918803374 1104 120-121 -0.2914479414032556 1104 122-123 -0.3500576380639693 1104 124-125 -0.38715946473819685 1105 1 0.3198447865258025 1105 2 0.14934516678741616 1105 3 0.13334234350513796 1105 4 0.1409469541355186 1105 5 0.09770842848249472 1105 6 0.4131358471099986 1105 7 0.5036008381297634 1105 8 0.3317854580229138 1105 9 0.22439571798357605 1105 10-11 0.05976916514347863 1105 12-13 0.19775184067189144 1105 14-15 0.16215940202882706 1105 16-17 0.3718210234326733 1105 18-19 0.36572436854103074 1105 20-21 0.3723987440124432 1105 22-23 0.25029474196769996 1105 24-25 0.13912948444836815 1105 26-27 0.3393387648092272 1105 28-29 0.33582801198074463 1105 30-31 0.4094243028437248 1105 32-33 0.36330039789900326 1105 34-35 0.4604756037449178 1105 36-37 0.37318802753191704 1105 38-39 0.4029135249208835 1105 40-41 0.2957344800602115 1105 42-43 0.20976401457100025 1105 44-45 0.3814944407021912 1105 46-47 0.23420435159691522 1105 48-49 0.20506945663884935 1105 50-51 0.10176345275550958 1105 52-53 0.17633065708264084 1105 54-55 0.2548528910999437 1105 56-57 0.2814761412389224 1105 58-59 0.1801612685980949 1105 60-61 0.22181012964378155 1105 62-63 0.14922205967704372 1105 64-65 0.21530864599769117 1105 66-67 -0.0013141786827368662 1105 68-69 -0.029403784942665823 1105 70-71 0.08096679603987056 1105 72-73 0.2154264653249527 1105 74-75 -0.049692567061210724 1105 76-77 0.010086425496652396 1105 78-79 -0.12029967012096776 1105 80-81 -0.2807476595724552 1105 82-83 0.06903317990825641 1105 84-85 0.02417535888275424 1105 86-87 -0.009582387549396998 1105 88-89 -0.007042187408899281 1105 90-91 0.045323651430607015 1105 92-93 0.14367901086693635 1105 94-95 -0.01652568876327365 1105 96-97 0.10396927789450672 1105 98-99 -0.021873548543528187 1105 100-101 0.0031586290791771887 1105 102-103 0.11090006500950267 1105 104-105 0.0669958323953459 1105 106-107 -0.07849839189879759 1105 108-109 -0.008218047889624103 1105 110-111 -0.22888364012067441 1105 112-113 0.011898955800635491 1105 114-115 -0.004629659781290485 1105 116-117 0.1224143269491087 1105 118-119 -0.021815818730615888 1105 120-121 -0.06105883043362681 1105 122-123 -0.17183007430707775 1105 124-125 -0.3374877151478586 1106 1 0.5448342798820285 1106 2 0.3166201010650056 1106 3 0.14590627755024244 1106 4 0.0758318057906493 1106 5 -0.07792498800980496 1106 6 0.19857486833789295 1106 7 0.40507445080569227 1106 8 0.16453835517404514 1106 9 0.11383207007685314 1106 10-11 -0.008138906194396611 1106 12-13 0.17121569111093748 1106 14-15 0.29724633891742513 1106 16-17 0.3121237824326215 1106 18-19 0.16156804085893128 1106 20-21 0.2861608266527895 1106 22-23 0.3657686251338177 1106 24-25 0.2552272834182716 1106 26-27 0.322662485681235 1106 28-29 0.33033387558216276 1106 30-31 0.0920608445036919 1106 32-33 0.1934110417997843 1106 34-35 0.30670539522157014 1106 36-37 0.27047681046018823 1106 38-39 0.096325803387181 1106 40-41 0.19946417094134006 1106 42-43 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0.45889184273809036 2314 120-121 0.6404628235151044 2314 122-123 0.7424924549151761 2314 124-125 0.6062736189081797 2315 1 0.34934808216023683 2315 2 0.09710255720535699 2315 3 0.03564083313351318 2315 4 -0.08552545287734858 2315 5 -0.26205600571995546 2315 6 -0.45382857807435073 2315 7 -0.6554934381109092 2315 8 -0.5686957223182603 2315 9 -0.6813360932223915 2315 10-11 -0.6146027479670764 2315 12-13 -0.7705006034762434 2315 14-15 -0.6194281227105805 2315 16-17 -0.6004326011210566 2315 18-19 -0.6014887792125805 2315 20-21 -0.5898673287475589 2315 22-23 -0.6033464388887992 2315 24-25 -0.6099248222606164 2315 26-27 -0.2895963901814085 2315 28-29 -0.3700436603785917 2315 30-31 -0.4498854014429341 2315 32-33 -0.3423067702065339 2315 34-35 -0.5043920314018635 2315 36-37 -0.5200343831042957 2315 38-39 -0.5258215005955194 2315 40-41 -0.5380386872111487 2315 42-43 -0.2729204177634159 2315 44-45 -0.18597226074859208 2315 46-47 -0.16902312367173522 2315 48-49 -0.07312178517037893 2315 50-51 -0.18492111324011873 2315 52-53 -0.05974462316303786 2315 54-55 -0.017242071160897154 2315 56-57 0.03004713385856661 2315 58-59 0.09557747832058183 2315 60-61 -0.06108743595085997 2315 62-63 0.0264153917542842 2315 64-65 0.09112269105279225 2315 66-67 -0.026008086469392566 2315 68-69 -0.04042539940899559 2315 70-71 0.07625483593854554 2315 72-73 0.07698365058099554 2315 74-75 0.1348621559299623 2315 76-77 0.13710905772059334 2315 78-79 0.08012611328668484 2315 80-81 0.20834627043738152 2315 82-83 -0.08538738266048895 2315 84-85 0.06181809858319198 2315 86-87 -0.043505158748789796 2315 88-89 0.25861509559696216 2315 90-91 0.09094922613353162 2315 92-93 0.10248720381574472 2315 94-95 0.02983945110592856 2315 96-97 0.053974954469818925 2315 98-99 0.1301845924706555 2315 100-101 0.08966359789424772 2315 102-103 0.07477550725900528 2315 104-105 -0.1289186840167993 2315 106-107 0.07592961865882941 2315 108-109 0.03257841185983423 2315 110-111 -0.03963889840082757 2315 112-113 0.16740511307317618 2315 114-115 0.09144818901406637 2315 116-117 0.1134209810226352 2315 118-119 0.05912872314157269 2315 120-121 0.2417276836338118 2315 122-123 0.43407650295151257 2315 124-125 0.4471242969686102 2316 1 -0.8612848485842974 2316 2 -1.1684888406441054 2316 3 -0.7471115492748837 2316 4 -0.3290458116400252 2316 5 -0.29134816288247123 2316 6 -1.426258889443659 2316 7 -1.5200528836595026 2316 8 -1.4080882718774532 2316 9 -1.5811806633696648 2316 10-11 -1.5415023046097858 2316 12-13 -1.3559118555783627 2316 14-15 -1.1782736101487927 2316 16-17 -1.293169440544176 2316 18-19 -1.2657080627064303 2316 20-21 -1.0311301326001825 2316 22-23 -1.225988236779024 2316 24-25 -1.333692849575506 2316 26-27 -0.9953128963221616 2316 28-29 -0.9230210338688849 2316 30-31 -0.804706274907744 2316 32-33 -1.1017166463722603 2316 34-35 -1.179765317851441 2316 36-37 -1.0806214493021216 2316 38-39 -1.0794116753904035 2316 40-41 -1.0486028211356242 2316 42-43 -0.7375308239194567 2316 44-45 -0.7235760929862778 2316 46-47 -0.439704212190307 2316 48-49 -0.6528037909798883 2316 50-51 -0.4820902514962455 2316 52-53 -0.3867906915521715 2316 54-55 -0.5705354004173842 2316 56-57 -0.33680808705566534 2316 58-59 -0.2085758111866305 2316 60-61 -0.245037392634341 2316 62-63 -0.06118207040746526 2316 64-65 -0.16459254107277488 2316 66-67 0.002268680589672556 2316 68-69 -0.0363001382358874 2316 70-71 0.030376275066100078 2316 72-73 0.07692504588242599 2316 74-75 0.30088671894275265 2316 76-77 0.2322384976635199 2316 78-79 0.17371017280867207 2316 80-81 0.019118068837215674 2316 82-83 0.20502947510845715 2316 84-85 0.15080168926759185 2316 86-87 0.11196546246101491 2316 88-89 0.07651118691837056 2316 90-91 -0.08867035293184955 2316 92-93 0.07303324672457379 2316 94-95 0.06502265448971656 2316 96-97 0.06260513334155249 2316 98-99 0.22931571411362484 2316 100-101 0.2203316914579183 2316 102-103 -0.09605081899080403 2316 104-105 -0.19283092985077133 2316 106-107 0.18278891642165362 2316 108-109 0.05728538836699215 2316 110-111 0.27831961966470686 2316 112-113 0.04487797654970649 2316 114-115 -0.21157122697737307 2316 116-117 -0.15807929926070585 2316 118-119 0.1089236066965853 2316 120-121 -0.10158501065350478 2316 122-123 0.1672136379461584 2316 124-125 0.21344697953498581 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 9.0 11 33.0 12 95.0 13 280.0 14 613.0 15 3836.0 16 8813.0 17 13235.0 18 21253.0 19 33241.0 20 54550.0 21 88405.0 22 137634.0 23 190717.0 24 181181.0 25 116843.0 26 82040.0 27 70141.0 28 63644.0 29 66525.0 30 75054.0 31 87486.0 32 105945.0 33 132594.0 34 169399.0 35 219743.0 36 326254.0 37 493677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.385244947390966 13.842537495634314 9.300289512973707 19.471928044001007 2 14.794434345356716 23.075540447028644 37.40330214647542 24.72672306113922 3 20.31122299818973 36.06973063258728 24.712074253747936 18.90697211547505 4 13.01862031758067 18.76241232994561 34.59646257709861 33.622504775375106 5 12.547207028387295 39.606797082880924 29.66384293467838 18.182152954053404 6 27.333242929351474 33.993683386299864 21.328814592369174 17.344259091979488 7 25.86323044047882 36.12375430239111 21.64187483277086 16.371140424359208 8 29.45711679829924 27.807718021231814 22.032799147869564 20.702366032599386 9 33.999771073787876 14.295530616693677 20.560891091635373 31.143807217883072 10-11 23.317848006118307 27.549793273797935 25.893632424700407 23.238726295383348 12-13 27.156335460014102 26.103238431024312 28.453067574789248 18.28735853417234 14-15 26.296732114775146 23.274869661517286 30.311908318697366 20.116489905010205 16-17 19.59064114308586 27.962198727709303 30.067901434835658 22.379258694369177 18-19 19.93842672275129 25.926191900039207 35.8078079698225 18.327573407387007 20-21 22.236522059285896 25.34041500310309 34.83882609293963 17.584236844671388 22-23 20.57894668732107 27.9044983478481 32.07385571917592 19.44269924565491 24-25 19.056255951053 29.29195774879958 32.96416755248956 18.687618747657854 26-27 20.05565389960061 28.19261710029586 33.09207044025891 18.65965855984462 28-29 19.588180742471067 29.090888875765614 33.529854353536614 17.791076028226705 30-31 20.998460029574577 29.103417881336796 32.46959018632931 17.428531902759314 32-33 17.815890832817523 30.0847136344515 33.13612142129641 18.963274111434572 34-35 17.133249636743678 31.43623493661879 32.55064627911063 18.879869147526904 36-37 19.09020421822063 31.595553728034204 31.002040651171132 18.312201402574036 38-39 18.8799734036106 30.99500060056951 30.906309515846196 19.218716479973697 40-41 17.891322673690063 31.827501515103187 30.69804107410563 19.58313473710112 42-43 18.896250833132953 31.389688625415197 30.39223305231572 19.32182748913613 44-45 19.227915728613578 30.586387239284623 30.57384533084494 19.61185170125686 46-47 18.02082245916508 30.09179367238129 31.596903487845097 20.290480380608532 48-49 17.98560467074789 30.081981630468686 31.21458167939657 20.71783201938685 50-51 17.059850588829654 29.502388797800872 31.087521477604323 22.350239135765154 52-53 17.517322416092362 28.39914416027996 30.516142445638252 23.567390977989426 54-55 18.013803434182076 27.63380305384159 30.688302920722414 23.664090591253924 56-57 18.712734359699795 26.08332874660544 30.92102532294374 24.282911570751025 58-59 18.144401608367403 26.17521213184019 31.81755427435395 23.862831985438458 60-61 18.72828963720364 25.802020329120552 31.379790088122604 24.089899945553203 62-63 17.596696894294986 25.984891184561416 32.25690765254113 24.161504268602464 64-65 17.371957435648138 25.831130385608216 32.117935341426715 24.67897683731693 66-67 18.146309020200064 25.27562377868663 31.863778847597835 24.71428835351547 68-69 18.57291220182642 24.809227266382635 31.96389308275164 24.653967449039307 70-71 17.931501346955557 24.67703429798081 32.06723656812405 25.32422778693958 72-73 18.72671336325754 24.352053248234885 31.642351648267226 25.27888174024035 74-75 18.978617209114798 23.87682174836622 31.41214813557313 25.732412906945857 76-77 18.473348855382984 24.10734799781657 30.86851282471547 26.55079032208497 78-79 18.942448371662522 23.79685213960484 30.26395953211201 26.996739956620626 80-81 18.396001969310657 24.09141708771225 29.35516077661251 28.157420166364577 82-83 18.850238315466548 23.82866875854891 27.92246691060961 29.39862601537493 84-85 19.589924587960315 23.28133257010007 27.383277715518727 29.745465126420896 86-87 20.023505782946422 22.495257725690042 26.992016202396623 30.489220288966916 88-89 20.55146395364846 22.41072174614345 26.480194202839723 30.557620097368364 90-91 21.425020567519525 22.347582967815136 26.008701705978442 30.218694758686894 92-93 21.139436683684625 22.778920880984504 25.4682097435195 30.613432691811372 94-95 21.28228205896527 22.53911612610189 25.998034442458962 30.18056737247388 96-97 22.04003511174005 22.262345064708107 25.631479103921563 30.066140719630276 98-99 22.54715406077918 22.37482147036244 25.523996789200513 29.554027679657864 100-101 22.695190581071593 22.24739924512675 25.819395445243252 29.238014728558397 102-103 22.567367370432503 22.749906862026755 26.354492239474315 28.328233528066427 104-105 22.51966833403688 22.606262954562524 27.076448231690826 27.79762047970977 106-107 21.57469155109174 23.31434849714316 27.738693852018887 27.372266099746213 108-109 21.274736242737607 23.762467912054422 28.0141161443285 26.94867970087947 110-111 20.833889244915323 24.121331621490192 28.713671633782216 26.33110749981227 112-113 20.055450098015267 24.619832565312784 28.65074470792399 26.673972628747954 114-115 20.016241357746843 24.599505786690994 29.072340664760343 26.311912190801824 116-117 19.787355242463683 24.929442992511458 29.450274991179803 25.832926773845056 118-119 19.31203833457457 25.872151536757222 29.52928717772498 25.286522950943223 120-121 19.106731948958927 26.56930166452326 29.600095773641332 24.723870612876482 122-123 18.369756090973773 27.65010940787266 29.42406622830524 24.556068272848325 124-125 17.697298176122157 28.90242893299519 29.8203644547778 23.57990843610486 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 84.0 1 52.0 2 41.5 3 92.5 4 146.5 5 306.5 6 591.5 7 1075.0 8 2201.5 9 3166.5 10 3361.5 11 3672.5 12 4186.0 13 4745.0 14 5394.5 15 6222.5 16 7098.5 17 8082.0 18 9259.0 19 10446.0 20 12040.5 21 13848.0 22 15787.5 23 18351.5 24 21237.5 25 24550.0 26 28694.5 27 33559.0 28 38765.0 29 44710.0 30 51299.0 31 57653.5 32 63877.5 33 68668.5 34 71496.5 35 71725.0 36 70422.5 37 68832.0 38 65696.5 39 61201.5 40 56302.0 41 52078.0 42 49039.5 43 48750.0 44 51083.5 45 53853.0 46 59323.0 47 66482.0 48 86584.0 49 106657.5 50 131897.0 51 140872.0 52 128541.0 53 134672.0 54 126212.0 55 100586.0 56 81555.5 57 66318.5 58 53423.0 59 42739.5 60 32604.0 61 25561.0 62 19912.0 63 14160.0 64 9527.0 65 6917.5 66 5105.0 67 3648.5 68 2777.0 69 2262.5 70 1962.0 71 1709.0 72 1478.0 73 1524.5 74 1216.5 75 849.5 76 690.0 77 497.5 78 358.0 79 209.5 80 158.5 81 150.0 82 125.5 83 103.0 84 77.5 85 51.5 86 28.0 87 8.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1138434013477484 2 0.015310351766267794 3 0.0 4 7.29064369822276E-5 5 0.019538925111236997 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.64532184911138E-5 18-19 2.369459201922397E-4 20-21 3.463055756655811E-4 22-23 5.4679827736670705E-5 24-25 0.001093596554733414 26-27 0.0013852223026623244 28-29 8.748772437867313E-4 30-31 2.733991386833535E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.5566523113892246E-4 40-41 0.0010753699454878571 42-43 0.3220459587597449 44-45 0.30660802072875815 46-47 0.3046942267579747 48-49 0.31367994511603425 50-51 0.3174164000113734 52-53 0.32266566347409376 54-55 0.3226109836463571 56-57 0.3245612308356317 58-59 0.3250351226760162 60-61 0.34194941605589296 62-63 0.33569768908466696 64-65 0.3428425199089253 66-67 0.3558016390825162 68-69 0.3675031222181638 70-71 0.35250262280907046 72-73 0.3565307034523385 74-75 0.3651154364069958 76-77 0.3618346467427956 78-79 0.43707408970845446 80-81 0.5978145566450208 82-83 0.5670298136292751 84-85 0.5285716681211501 86-87 0.48393470207878125 88-89 0.3126045751705464 90-91 0.01627636205628231 92-93 0.07008131254916627 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.1597744566465518 114-115 0.14358922763649726 116-117 0.13817592469056686 118-119 0.1226833068318435 120-121 0.12570892396660593 122-123 0.1190379849827321 124-125 7.655175883133897E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2743242.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.16325616074587 #Duplication Level Percentage of deduplicated Percentage of total 1 74.23062351961856 26.844210533118535 2 13.541097397722021 9.793803477828614 3 5.111458328459746 5.54540930661203 4 2.2358847747108808 3.2342749541512457 5 1.1500469721054107 2.079472162456906 6 0.696593769258088 1.511465935059584 7 0.46098198208501917 1.1669426652540238 8 0.34177054135438184 0.988762850815624 9 0.25806130149191336 0.8399103255924779 >10 1.629238389643557 11.245321238951945 >50 0.17112277095726772 4.340261906997014 >100 0.1406132434696701 10.124967229686803 >500 0.016874657602297626 4.208090466527734 >1k 0.014079468919708446 9.57006351656319 >5k 8.282040541004969E-4 2.359617065985565 >10k+ 7.246785473379347E-4 6.147426364398777 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60787 2.2158817924193346 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 23372 0.8519846225743116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23150 0.8438920080692844 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 20013 0.7295382616626604 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15695 0.572133264218031 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 10782 0.39303860177118893 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 10403 0.37922283196305684 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9365 0.3413843911692807 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 9189 0.3349686247148447 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9165 0.33409374747105797 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 9012 0.32851640504191754 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8147 0.2969843710471041 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 7512 0.27383657730524685 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 5571 0.20308088021399498 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5066 0.1846720048759825 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4890 0.17825623842154648 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 4781 0.17428283760601507 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4649 0.16947101276518806 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4558 0.1661537698824967 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 4406 0.1606128806718474 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 4403 0.16050352101637405 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 4390 0.16002962917598956 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 4186 0.15259317260380237 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 4186 0.15259317260380237 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 3931 0.14329760188856835 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3657 0.13330942002200316 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 3533 0.12878922092910505 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 3432 0.12510744586150255 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 3299 0.12025916780218442 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 3134 0.11424438675115063 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3093 0.11274980479301498 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 2952 0.10760990098576795 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 2921 0.10647985121254341 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 2884 0.1051310821283722 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 2846 0.10374585982570989 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 2827 0.1030532486743787 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.64532184911138E-5 0.0 6 1.093596554733414E-4 0.0 0.0 3.64532184911138E-5 0.0 7 1.093596554733414E-4 0.0 0.0 7.29064369822276E-5 0.0 8 1.093596554733414E-4 0.0 0.0 7.29064369822276E-5 0.0 9 1.093596554733414E-4 0.0 0.0 7.29064369822276E-5 0.0 10-11 1.093596554733414E-4 0.0 0.0 1.82266092455569E-4 0.0 12-13 1.2758626471889829E-4 0.0 0.0 4.921184496300363E-4 0.0 14-15 1.458128739644552E-4 0.0 0.0 6.743845420856052E-4 0.0 16-17 1.82266092455569E-4 0.0 0.0 0.001439902130398995 0.0 18-19 1.82266092455569E-4 0.0 0.0 0.002497045466641295 0.0 20-21 1.82266092455569E-4 0.0 0.0 0.0056320222568770825 1.82266092455569E-5 22-23 1.82266092455569E-4 0.0 0.0 0.018244835854802455 3.64532184911138E-5 24-25 1.82266092455569E-4 0.0 0.0 0.04764435656788574 3.64532184911138E-5 26-27 1.82266092455569E-4 0.0 0.0 0.08819856213924984 3.64532184911138E-5 28-29 1.82266092455569E-4 0.0 0.0 0.19518875841066885 3.64532184911138E-5 30-31 1.82266092455569E-4 0.0 0.0 0.5087228906527386 3.64532184911138E-5 32-33 1.82266092455569E-4 0.0 0.0 1.049743332888604 3.64532184911138E-5 34-35 1.82266092455569E-4 0.0 0.0 1.4447321818490675 3.64532184911138E-5 36-37 1.82266092455569E-4 0.0 0.0 1.917384612804849 3.64532184911138E-5 38-39 1.82266092455569E-4 0.0 0.0 2.493837583414077 3.64532184911138E-5 40-41 1.82266092455569E-4 0.0 0.0 3.367220245242673 3.64532184911138E-5 42-43 1.82266092455569E-4 0.0 0.0 4.115240288680328 3.64532184911138E-5 44-45 1.82266092455569E-4 0.0 0.0 4.755249445728813 3.64532184911138E-5 46-47 2.369459201922397E-4 0.0 0.0 5.393381262025006 3.64532184911138E-5 48-49 2.916257479289104E-4 0.0 0.0 5.958807134040672 3.64532184911138E-5 50-51 2.916257479289104E-4 0.0 0.0 6.535405917523864 3.64532184911138E-5 52-53 2.916257479289104E-4 0.0 0.0 7.214420018357841 3.64532184911138E-5 54-55 2.916257479289104E-4 0.0 0.0 7.831937539597309 3.64532184911138E-5 56-57 2.916257479289104E-4 0.0 0.0 8.422024013922213 3.64532184911138E-5 58-59 2.916257479289104E-4 0.0 0.0 9.051006072377136 3.64532184911138E-5 60-61 2.916257479289104E-4 0.0 0.0 9.62665707217956 3.64532184911138E-5 62-63 3.64532184911138E-4 0.0 0.0 10.198444030821925 3.64532184911138E-5 64-65 3.64532184911138E-4 0.0 0.0 10.810019677447341 3.64532184911138E-5 66-67 4.009854034022518E-4 0.0 0.0 11.392615015372321 3.64532184911138E-5 68-69 4.009854034022518E-4 0.0 0.0 12.043760630669842 3.64532184911138E-5 70-71 4.009854034022518E-4 0.0 0.0 12.798068854297213 3.64532184911138E-5 72-73 5.103450588755931E-4 3.64532184911138E-5 0.0 13.443309048199175 3.64532184911138E-5 74-75 5.103450588755931E-4 3.64532184911138E-5 0.0 14.050619668261131 3.64532184911138E-5 76-77 5.103450588755931E-4 3.64532184911138E-5 0.0 14.670069210080626 3.64532184911138E-5 78-79 5.103450588755931E-4 3.64532184911138E-5 0.0 15.262652729872173 3.64532184911138E-5 80-81 5.103450588755931E-4 3.64532184911138E-5 0.0 15.881591926632794 3.64532184911138E-5 82-83 5.103450588755931E-4 3.64532184911138E-5 0.0 16.590005548179853 3.64532184911138E-5 84-85 5.103450588755931E-4 3.64532184911138E-5 0.0 17.204315186192105 3.64532184911138E-5 86-87 5.103450588755931E-4 3.64532184911138E-5 0.0 17.796588853626474 3.64532184911138E-5 88-89 5.103450588755931E-4 3.64532184911138E-5 0.0 18.386821140825347 3.64532184911138E-5 90-91 5.103450588755931E-4 3.64532184911138E-5 0.0 18.952884944164605 3.64532184911138E-5 92-93 5.103450588755931E-4 3.64532184911138E-5 0.0 19.505406376834415 3.64532184911138E-5 94-95 5.103450588755931E-4 3.64532184911138E-5 0.0 20.07347510719069 3.64532184911138E-5 96-97 5.103450588755931E-4 3.64532184911138E-5 0.0 20.644660587727948 3.64532184911138E-5 98-99 5.103450588755931E-4 3.64532184911138E-5 0.0 21.24559189455396 3.64532184911138E-5 100-101 5.103450588755931E-4 3.64532184911138E-5 0.0 21.915674956857615 3.64532184911138E-5 102-103 5.2857166812115E-4 3.64532184911138E-5 0.0 22.498179161736367 3.64532184911138E-5 104-105 6.014781051033777E-4 3.64532184911138E-5 0.0 23.050354288830516 7.29064369822276E-5 106-107 6.561579328400484E-4 3.64532184911138E-5 0.0 23.592213155091677 7.29064369822276E-5 108-109 6.561579328400484E-4 3.64532184911138E-5 0.0 24.13348876985698 7.29064369822276E-5 110-111 6.561579328400484E-4 3.64532184911138E-5 0.0 24.66255255642776 7.29064369822276E-5 112-113 6.561579328400484E-4 3.64532184911138E-5 0.0 25.239333606003406 7.29064369822276E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 745 0.0 65.43324 1 TACAACG 700 0.0 56.84053 2 TAGTACT 1040 0.0 50.81484 5 ATAACGC 785 0.0 49.923855 3 TCACGCA 600 0.0 48.492958 4 GCATAGT 1095 0.0 47.1831 2 ATAGTAC 1125 0.0 46.975494 4 AGATTAC 1255 0.0 46.372845 2 GTACAAC 955 0.0 46.064804 1 ACAACGC 925 0.0 43.651688 3 AGTGTAC 1420 0.0 43.48894 3 GGTTTCA 1640 0.0 42.773914 1 TAACGCA 940 0.0 41.691734 4 GACCGTT 495 0.0 40.778233 7 CATAGTA 1300 0.0 40.195107 3 TTCAACG 1850 0.0 39.800068 4 GATTACT 1500 0.0 39.58609 3 GTATAAC 935 0.0 39.42039 1 GTATCAC 890 0.0 38.741714 1 TACTGGT 1645 0.0 38.266727 2 >>END_MODULE