FastQCFastQC Report
Fri 10 Feb 2017
SRR3558098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8209283
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT976321.1892877855471666No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT967301.1783002242704996No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA856581.0434285186660028No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG357360.43531207293012075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353880.43107296946639556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352340.42919704437037925No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT344000.4190378136555897No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC322750.3931524835969232No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG287500.3502132890290175No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC273780.3335005018099632No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG195440.238071948548978No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182420.22221185455538564No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA181180.22070136941313875No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC180090.21937360424777655No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC174420.21246678912153474No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG145270.17695820694694045No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC132390.16126865159844045No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT121510.1480153626084032No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG113630.13841647315606004No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT108840.1325816152275418No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA107740.13124166873038728No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC104260.12700256526666215No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC98630.12014447546758956No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA97900.11925523824675066No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC97830.11916996892420445No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC96000.11694078520621107No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG93450.11383454559917108No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA90330.11003397007996922No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA89030.10845039694696845No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT85760.1044671014508819No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83420.10161666981148049No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC83060.10117814186695719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG15950.045.4813541
TACAACG13450.029.5854642
TAACGCA16700.029.5115664
TAGTACT25750.029.0552065
CATGGGG241550.028.9334414
ATAGTAC25950.028.8312764
GACCGTT14600.027.6512837
GTACTGG56850.026.8804741
CATGGGT112050.025.9135934
ATGGGTG61750.025.4823575
TACTGGT50400.024.864392
GTATAAC19800.024.625381
GCATAGT29500.024.5619982
GAGTACT591000.024.51103212-13
ATAACGC24150.024.0957033
GGACCGT25700.024.024786
AGTACTT672850.023.82786812-13
AGATTAC30650.023.446652
GGTTCAC52400.022.9997966
CATGGGC253250.022.461914
Produced by FastQC (version 0.11.5)