##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3558083.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574056 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.721696419969106 33.0 32.0 34.0 27.0 34.0 2 31.95136352899859 33.0 32.0 34.0 27.0 34.0 3 32.08924592161165 33.0 32.0 34.0 28.0 34.0 4 31.937195228075847 33.0 32.0 34.0 28.0 34.0 5 32.10444799957732 33.0 32.0 34.0 30.0 34.0 6 35.458800041646334 38.0 36.0 38.0 29.0 38.0 7 35.797102704836256 38.0 36.0 38.0 31.0 38.0 8 36.00206483464229 38.0 37.0 38.0 31.0 38.0 9 36.11491397234559 38.0 37.0 38.0 33.0 38.0 10-11 36.10267647634706 38.0 37.0 38.0 32.0 38.0 12-13 36.09373941359473 38.0 37.0 38.0 32.0 38.0 14-15 36.093016818592915 38.0 37.0 38.0 32.0 38.0 16-17 36.0552925033488 38.0 37.0 38.0 32.0 38.0 18-19 36.03126524830851 38.0 37.0 38.0 31.0 38.0 20-21 35.99825100930205 38.0 37.0 38.0 31.0 38.0 22-23 35.951577393809615 38.0 37.0 38.0 31.0 38.0 24-25 35.85813284559465 38.0 37.0 38.0 31.0 38.0 26-27 35.744143872549785 38.0 37.0 38.0 30.0 38.0 28-29 35.65502867847475 38.0 37.0 38.0 29.0 38.0 30-31 35.54629289339471 38.0 37.0 38.0 29.0 38.0 32-33 35.426341151863056 38.0 37.0 38.0 28.5 38.0 34-35 35.22497917683221 38.0 36.0 38.0 27.0 38.0 36-37 35.04698246658192 38.0 36.0 38.0 26.5 38.0 38-39 34.85496974424799 38.0 36.0 38.0 25.0 38.0 40-41 34.59261298122496 38.0 35.5 38.0 24.5 38.0 42-43 34.13884701809129 38.0 35.0 38.0 16.0 38.0 44-45 33.778804346136994 38.0 34.0 38.0 15.5 38.0 46-47 33.2271026737569 38.0 33.5 38.0 15.0 38.0 48-49 32.75412966928458 38.0 31.5 38.0 15.0 38.0 50-51 32.368327456745305 38.0 29.0 38.0 15.0 38.0 52-53 32.062656562250396 38.0 27.5 38.0 15.0 38.0 54-55 31.855374164353847 38.0 26.0 38.0 15.0 38.0 56-57 31.64948548127935 38.0 26.0 38.0 15.0 38.0 58-59 31.49679766096775 38.0 25.0 38.0 15.0 38.0 60-61 31.3393348862651 38.0 25.0 38.0 15.0 38.0 62-63 31.21708268973169 38.0 25.0 38.0 15.0 38.0 64-65 31.116666847962904 38.0 24.5 38.0 15.0 38.0 66-67 30.93859146809549 38.0 24.0 38.0 15.0 38.0 68-69 30.81986114521207 38.0 24.0 38.0 15.0 38.0 70-71 30.625460557190678 37.5 24.0 38.0 14.0 38.0 72-73 30.446803604894377 37.0 20.0 38.0 14.0 38.0 74-75 30.29870309736851 37.0 16.0 38.0 14.0 38.0 76-77 30.202774531711817 37.0 16.0 38.0 14.0 38.0 78-79 29.99908782093319 37.0 15.0 38.0 14.0 38.0 80-81 29.91763893248632 37.0 15.0 38.0 14.0 38.0 82-83 29.798289159210213 37.0 15.0 38.0 14.0 38.0 84-85 29.69547379699587 37.0 15.0 38.0 13.0 38.0 86-87 29.58241837007431 37.0 15.0 38.0 13.0 38.0 88-89 29.4784723020789 36.5 15.0 38.0 13.0 38.0 90-91 29.462283454594616 37.0 15.0 38.0 13.0 38.0 92-93 29.40459628694947 37.0 15.0 38.0 13.0 38.0 94-95 29.337576960252612 36.0 15.0 38.0 13.0 38.0 96-97 29.27287926136805 36.0 15.0 38.0 13.0 38.0 98-99 29.17303586246764 36.0 14.0 38.0 13.0 38.0 100-101 29.10004929962674 36.0 14.0 38.0 13.0 38.0 102-103 29.01979288717883 36.0 14.0 38.0 13.0 38.0 104-105 28.85033775488956 35.5 14.0 38.0 13.0 38.0 106-107 28.713926969731816 35.0 14.0 38.0 13.0 38.0 108-109 28.595565908434004 35.0 14.0 38.0 13.0 38.0 110-111 28.4658787532206 35.0 14.0 38.0 13.0 38.0 112-113 28.149186925226182 34.0 14.0 38.0 13.0 38.0 114-115 28.02511988861159 34.0 14.0 38.0 13.0 38.0 116-117 27.906705603918486 34.0 14.0 38.0 13.0 38.0 118-119 27.72412430032602 34.0 14.0 38.0 13.0 38.0 120-121 27.57607177155431 34.0 14.0 38.0 13.0 38.0 122-123 27.398440243724302 33.0 14.0 38.0 13.0 38.0 124-125 26.31089300310483 28.5 14.0 37.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33500201428831033 1101 2 -0.6348221693009108 1101 3 -0.23629342324841573 1101 4 -0.38037572342548387 1101 5 -0.04983791998539999 1101 6 0.04856007994096245 1101 7 0.058954948460460344 1101 8 0.08843796522556602 1101 9 0.06114822288284927 1101 10-11 0.10561371337976766 1101 12-13 0.2069182217836314 1101 14-15 0.2291619595647063 1101 16-17 0.1717230472767426 1101 18-19 0.1011832358028002 1101 20-21 0.14702856291322064 1101 22-23 0.05719394363436692 1101 24-25 -0.06816267249292451 1101 26-27 0.04872715576221509 1101 28-29 0.10290165368128612 1101 30-31 -0.04157105329754529 1101 32-33 0.09978321155210068 1101 34-35 -0.05334951301840363 1101 36-37 0.06094756906211529 1101 38-39 0.17029407307200017 1101 40-41 0.13528838803656384 1101 42-43 -0.08349529427763969 1101 44-45 0.0823061577044939 1101 46-47 0.018485995791458265 1101 48-49 -0.1139619001612644 1101 50-51 0.05301085231651115 1101 52-53 -0.08341542648787481 1101 54-55 0.05680122959751088 1101 56-57 0.03541454334555638 1101 58-59 0.1237102711043967 1101 60-61 0.13410768918139127 1101 62-63 0.20650436639687086 1101 64-65 0.16542764770918694 1101 66-67 0.19085634839833077 1101 68-69 -0.03852178651195004 1101 70-71 -0.1034885682035096 1101 72-73 -0.057274765619734325 1101 74-75 -0.03054009956968784 1101 76-77 -0.2880518068458784 1101 78-79 -0.16869046232829987 1101 80-81 -0.18315219566942886 1101 82-83 -0.08466624658344202 1101 84-85 -0.1731435953167768 1101 86-87 -0.11308696727137502 1101 88-89 -0.11548852015163291 1101 90-91 0.1091611157960024 1101 92-93 0.24000849001836144 1101 94-95 0.21462360680471804 1101 96-97 -0.07575040235560238 1101 98-99 0.008561685531240926 1101 100-101 9.968864562921453E-4 1101 102-103 0.12315885120650805 1101 104-105 -0.03163219858609523 1101 106-107 0.12332098633473265 1101 108-109 0.023629185190753077 1101 110-111 -0.16166898792582884 1101 112-113 -0.47083969414023485 1101 114-115 -0.36428823916189756 1101 116-117 -0.19767211278233532 1101 118-119 -0.049706894045623784 1101 120-121 0.02536691788854384 1101 122-123 -0.25094467697636347 1101 124-125 -0.39294665203448886 1102 1 -0.7547723015970398 1102 2 -0.7378394331907323 1102 3 -0.4460228263236843 1102 4 -0.5262500902048046 1102 5 -0.2931536648916584 1102 6 -0.10192728548798868 1102 7 -0.1514860118713841 1102 8 -0.14396381562598748 1102 9 -0.3281360792128396 1102 10-11 -0.23641358132244505 1102 12-13 -0.1612399173869008 1102 14-15 -0.1964133946285358 1102 16-17 -0.19608840590409926 1102 18-19 -0.15850741864336015 1102 20-21 -0.15468733456285122 1102 22-23 -0.17621478865596885 1102 24-25 -0.31198900402824137 1102 26-27 -0.2001812613166578 1102 28-29 -0.2818608092953596 1102 30-31 -0.2990083653830169 1102 32-33 -0.2737680280310073 1102 34-35 -0.15927945213969252 1102 36-37 -0.07469175067795675 1102 38-39 0.007896983089388243 1102 40-41 -0.005360509282830606 1102 42-43 -0.2660206883852325 1102 44-45 -0.13625009914995445 1102 46-47 -0.22182987781059893 1102 48-49 -0.26560216553574634 1102 50-51 -0.2882922953218241 1102 52-53 -0.28997266441471936 1102 54-55 -0.24474237403432042 1102 56-57 -0.15247970413834722 1102 58-59 -0.13439639906876266 1102 60-61 -0.08264449491703019 1102 62-63 -0.21849395496215607 1102 64-65 -0.0692288743705376 1102 66-67 -0.12024734871515719 1102 68-69 -0.12892476292891075 1102 70-71 -0.13012533640930002 1102 72-73 -0.11163428948254506 1102 74-75 -0.2819007313355435 1102 76-77 -0.23856127438095953 1102 78-79 -0.17062020001267442 1102 80-81 -0.286454362235375 1102 82-83 -0.1938872545886916 1102 84-85 -0.22673840495889053 1102 86-87 -0.2839898709472273 1102 88-89 -0.0804384966506575 1102 90-91 -0.08227389841649213 1102 92-93 -0.1182305130181156 1102 94-95 -0.16418294474448203 1102 96-97 -0.09863074509678427 1102 98-99 -0.15176671259763452 1102 100-101 -0.17853912785318826 1102 102-103 -0.26020521058239865 1102 104-105 -0.224898796614994 1102 106-107 -0.01783569987877698 1102 108-109 -0.057020887177326784 1102 110-111 -0.26853010649756826 1102 112-113 -0.4386345007980985 1102 114-115 -0.1811844133526037 1102 116-117 0.01384527607298125 1102 118-119 -0.05960854433621421 1102 120-121 -0.08103280756039766 1102 122-123 -0.20930598765923136 1102 124-125 -0.6165868595891197 1103 1 -0.6954546189834758 1103 2 -0.6833627115637881 1103 3 -0.3872956438693045 1103 4 -0.31523781698568243 1103 5 -0.36821668290838616 1103 6 -0.16885181827323237 1103 7 -0.25233333028873517 1103 8 -0.07868673513767277 1103 9 -0.09226824892800778 1103 10-11 -0.11980289813903511 1103 12-13 -0.09276786428626593 1103 14-15 -0.07361378591193812 1103 16-17 -0.14933973183275384 1103 18-19 -0.10752893275850539 1103 20-21 -0.09551835566891498 1103 22-23 -0.12433645264597004 1103 24-25 -0.23569944999454862 1103 26-27 -0.19176994713636475 1103 28-29 -0.10920762366102821 1103 30-31 -0.13701669710602715 1103 32-33 -0.11374903402013814 1103 34-35 -0.13024663037796813 1103 36-37 -0.18989159769033392 1103 38-39 0.053600108209771236 1103 40-41 -0.027742961689170897 1103 42-43 -0.12336674459308483 1103 44-45 -0.22468181270683374 1103 46-47 -0.13992671983670846 1103 48-49 -0.19714035989351686 1103 50-51 -0.30739640857457573 1103 52-53 -0.25427976517913464 1103 54-55 -0.2774340777844593 1103 56-57 -0.26354466316238145 1103 58-59 -0.3706638802311595 1103 60-61 -0.2010531832824185 1103 62-63 -0.17524266909874697 1103 64-65 -0.14144895464280438 1103 66-67 -0.14216034424910973 1103 68-69 -0.22409360251904786 1103 70-71 -0.16884277684383164 1103 72-73 -0.30595091036695976 1103 74-75 -0.46451768257335857 1103 76-77 -0.48253230165707706 1103 78-79 -0.16762871264570478 1103 80-81 -0.34693432236736754 1103 82-83 -0.24272448395948132 1103 84-85 -0.4172743300824884 1103 86-87 -0.2818140196299872 1103 88-89 -0.23709447514116633 1103 90-91 -0.33189990470919994 1103 92-93 -0.1833949485125963 1103 94-95 -0.18784355570257105 1103 96-97 -0.21969645303281737 1103 98-99 -0.12908831667467524 1103 100-101 -0.33225786116254596 1103 102-103 -0.4426605909911707 1103 104-105 -0.4546390320199194 1103 106-107 -0.17085475522029014 1103 108-109 -0.40398295493875835 1103 110-111 -0.5732943733481655 1103 112-113 -0.6495570894940883 1103 114-115 -0.4710830082026156 1103 116-117 -0.3028138244251508 1103 118-119 -0.2728581536229697 1103 120-121 -0.1650366174575666 1103 122-123 -0.4437140559378179 1103 124-125 -0.505056450217797 1104 1 -0.31726969356073553 1104 2 -0.15482600605031038 1104 3 -0.10455474659386255 1104 4 -0.28492439956188775 1104 5 -0.16055512688118512 1104 6 0.12722917202541595 1104 7 0.14790554198462047 1104 8 0.15496655209278032 1104 9 0.08514456131622694 1104 10-11 0.15190468085744868 1104 12-13 0.1380770989854483 1104 14-15 0.12693625938878483 1104 16-17 0.10204803290435649 1104 18-19 0.08545911720653265 1104 20-21 0.04202522888901683 1104 22-23 0.033431773366579876 1104 24-25 0.06955627512743234 1104 26-27 0.10325695657199674 1104 28-29 0.08179066491025111 1104 30-31 0.039252663488660744 1104 32-33 0.011671675643334822 1104 34-35 0.04615314371466184 1104 36-37 0.10665593046170585 1104 38-39 0.10317000357625261 1104 40-41 0.08934894267508753 1104 42-43 9.308495301496578E-4 1104 44-45 0.09887850942214982 1104 46-47 0.07115486519641223 1104 48-49 0.026027406904525208 1104 50-51 -0.01745394951723256 1104 52-53 -0.034130314062487344 1104 54-55 -0.08696707515142421 1104 56-57 -0.003792393768762281 1104 58-59 0.027757841245357184 1104 60-61 0.21089156175689894 1104 62-63 0.14429967890072604 1104 64-65 0.16627063768429196 1104 66-67 0.21456254036903388 1104 68-69 0.16477743244430698 1104 70-71 0.00692916251343334 1104 72-73 0.0675421868909325 1104 74-75 0.12019758027023286 1104 76-77 0.019302507708356842 1104 78-79 0.1586771729016938 1104 80-81 0.23344924008907952 1104 82-83 0.08425227430648974 1104 84-85 0.14424116649633945 1104 86-87 0.14926603958049256 1104 88-89 0.2987076993548463 1104 90-91 0.2955922850052879 1104 92-93 0.2707377222243572 1104 94-95 0.31734385904323403 1104 96-97 0.34799281548047034 1104 98-99 0.1276895159049687 1104 100-101 0.33544785810382294 1104 102-103 0.2735482473440989 1104 104-105 0.27512485992248514 1104 106-107 0.18994649430308286 1104 108-109 0.21624904043056858 1104 110-111 0.12082764720793904 1104 112-113 -0.12808396430661162 1104 114-115 0.17722221258759063 1104 116-117 0.16855941900593763 1104 118-119 0.08316106379207966 1104 120-121 0.16719834271663103 1104 122-123 0.12700690596034292 1104 124-125 -0.16912213458899927 1105 1 -0.08586950507121927 1105 2 -0.1212838912701173 1105 3 -0.2410501643747054 1105 4 -0.22465480243943503 1105 5 -0.23446616403947473 1105 6 0.00578736252608536 1105 7 -0.0905841970611192 1105 8 -0.1171275303285455 1105 9 -0.17563338631254766 1105 10-11 -0.12494455308448238 1105 12-13 -0.07468464816503939 1105 14-15 -0.1328179977836612 1105 16-17 -0.09725828359869837 1105 18-19 -0.1475394743829881 1105 20-21 -0.21451403449876238 1105 22-23 -0.17591754700396223 1105 24-25 -0.20751060858776071 1105 26-27 -0.12401104274859165 1105 28-29 -0.04798616891685015 1105 30-31 -0.1937705038867179 1105 32-33 -0.1863360322851051 1105 34-35 -0.11980698789732003 1105 36-37 -0.1141328545625484 1105 38-39 -0.19610827999674996 1105 40-41 -0.05379794536906246 1105 42-43 -0.35032687455667855 1105 44-45 -0.2202583619224825 1105 46-47 -0.22996652716090438 1105 48-49 -0.2658039626206019 1105 50-51 -0.263431712564568 1105 52-53 -0.49617928826767965 1105 54-55 -0.3759357038225737 1105 56-57 -0.39193716994873995 1105 58-59 -0.3039495821450764 1105 60-61 -0.2348840335107205 1105 62-63 -0.3684721390610939 1105 64-65 -0.3965785150899137 1105 66-67 -0.44824805773194143 1105 68-69 -0.332020575639401 1105 70-71 -0.41846814602466154 1105 72-73 -0.3263586592075285 1105 74-75 -0.2973061662218974 1105 76-77 -0.5921172662545366 1105 78-79 -0.6270531638391823 1105 80-81 -0.5432268360222068 1105 82-83 -0.4711537221852602 1105 84-85 -0.6679016015337957 1105 86-87 -0.47395319296355964 1105 88-89 -0.38277688854605074 1105 90-91 -0.5114472121155131 1105 92-93 -0.6003591995645188 1105 94-95 -0.41725080583173124 1105 96-97 -0.4420709763453381 1105 98-99 -0.3171585125458236 1105 100-101 -0.39704719291911417 1105 102-103 -0.5108174193060329 1105 104-105 -0.5932439241698404 1105 106-107 -0.3824509900984374 1105 108-109 -0.3906460081873995 1105 110-111 -0.5381794158879494 1105 112-113 -0.6201755215095659 1105 114-115 -0.5809955694590094 1105 116-117 -0.4596954446698476 1105 118-119 -0.48450321443888456 1105 120-121 -0.4469420534119699 1105 122-123 -0.6978560483347103 1105 124-125 -0.6276291142624935 1106 1 0.20985555137946577 1106 2 0.23222370565284578 1106 3 -0.042619727354960446 1106 4 0.03039471682608763 1106 5 -0.12972600729579753 1106 6 0.17078538515738728 1106 7 -0.0045393171703196344 1106 8 0.052436300408693626 1106 9 -0.040405606195946575 1106 10-11 -0.04697137726027023 1106 12-13 -0.08976601794999084 1106 14-15 -0.023716786273247692 1106 16-17 -0.08541023056269381 1106 18-19 -0.0904441226877637 1106 20-21 -0.03010101533317311 1106 22-23 -0.022798194730405896 1106 24-25 -0.08675312754286324 1106 26-27 -0.05109248907685782 1106 28-29 -0.15263617465759438 1106 30-31 -0.04910897412625559 1106 32-33 -0.11629614471871008 1106 34-35 0.006364230969751361 1106 36-37 -0.11092165032375334 1106 38-39 -0.014373426003096768 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-0.1252695907319108 2315 26-27 -0.15443322159627826 2315 28-29 -0.04740495009230017 2315 30-31 -0.0582062734233304 2315 32-33 0.10976748828165483 2315 34-35 -0.16425360106872233 2315 36-37 -0.2622128082963755 2315 38-39 -0.08031127283994977 2315 40-41 -0.043508781774761474 2315 42-43 0.059405649118588144 2315 44-45 0.05258917657241824 2315 46-47 0.08888693918768809 2315 48-49 0.23881325707143475 2315 50-51 0.13269638690771046 2315 52-53 0.03647451061903695 2315 54-55 0.10013456293084388 2315 56-57 0.17620856850279054 2315 58-59 0.2526332270792402 2315 60-61 0.22267687157132698 2315 62-63 0.3304037374660531 2315 64-65 0.23593865399849534 2315 66-67 0.4243862226121671 2315 68-69 0.24002381097232828 2315 70-71 0.33048941921787645 2315 72-73 0.4755396997890706 2315 74-75 0.32684983753723174 2315 76-77 0.43570605478934255 2315 78-79 0.6931412986779897 2315 80-81 0.2755427728840303 2315 82-83 0.40263721253605667 2315 84-85 0.3890419392744171 2315 86-87 0.38674007675378164 2315 88-89 0.29495173142069575 2315 90-91 0.17309193340606654 2315 92-93 0.1586012573139577 2315 94-95 0.11178555649025412 2315 96-97 0.23005148443685286 2315 98-99 0.312571119493505 2315 100-101 0.34881921350031675 2315 102-103 0.43657866252726407 2315 104-105 0.2980218894642199 2315 106-107 0.21881626935359932 2315 108-109 0.3193445939957833 2315 110-111 0.2966250372314043 2315 112-113 0.4154653373063084 2315 114-115 0.3715136476305574 2315 116-117 0.4332005287270988 2315 118-119 0.3516610304826777 2315 120-121 0.13348012543571386 2315 122-123 0.3297157003821276 2315 124-125 0.4069432850724297 2316 1 -0.33001092896143547 2316 2 -0.11230633286819014 2316 3 0.09281644223935359 2316 4 0.32621098151518524 2316 5 0.20081229162806835 2316 6 -0.17444537196632837 2316 7 -0.3809976868376168 2316 8 -0.3111621004085592 2316 9 -0.2582573482220383 2316 10-11 -0.2985697390254316 2316 12-13 -0.37657086685764085 2316 14-15 -0.18126168544269206 2316 16-17 -0.2641130742494866 2316 18-19 -0.29985847015633027 2316 20-21 -0.2945467723614783 2316 22-23 -0.2404469842712018 2316 24-25 -0.16212194413017755 2316 26-27 -0.1522202954459857 2316 28-29 -0.14668553974323117 2316 30-31 -0.030043274471971415 2316 32-33 0.045136359601052334 2316 34-35 -0.06915820526477745 2316 36-37 -0.14668588698942386 2316 38-39 -0.09465851507513179 2316 40-41 -0.13761966572306505 2316 42-43 -0.08946861249751237 2316 44-45 0.030771111345679003 2316 46-47 -0.13376324602124612 2316 48-49 -0.2152260328729625 2316 50-51 -0.1038515599512877 2316 52-53 -0.03047606764022248 2316 54-55 0.0011844825856250907 2316 56-57 -0.0371280591103158 2316 58-59 0.02455457174891862 2316 60-61 -0.06597627094524938 2316 62-63 0.047053681262120506 2316 64-65 -0.04264093303461536 2316 66-67 0.07067525416935538 2316 68-69 -0.12844721571133988 2316 70-71 -0.006971671817410652 2316 72-73 0.15637483743212854 2316 74-75 -0.20030458371313742 2316 76-77 -0.22681105157565895 2316 78-79 -0.05903707525545698 2316 80-81 -0.04588178936653975 2316 82-83 -0.3263817459182974 2316 84-85 -0.07283104737997803 2316 86-87 -0.19927057558905403 2316 88-89 -0.12658848268234024 2316 90-91 -0.16414245585709963 2316 92-93 -0.21989866347244558 2316 94-95 -0.23222634818183963 2316 96-97 -0.31263347931350083 2316 98-99 -0.341997619048815 2316 100-101 -0.29321883920312786 2316 102-103 -0.19665166798204226 2316 104-105 -0.4626374694337301 2316 106-107 -0.2817667427245887 2316 108-109 -0.4754099570139978 2316 110-111 -0.22457252148096174 2316 112-113 -0.4096583490682022 2316 114-115 -0.43968496726310846 2316 116-117 -0.4334209736153589 2316 118-119 -0.7148215085771703 2316 120-121 -0.4302282025538453 2316 122-123 -0.6483168601785501 2316 124-125 -0.5612980221782564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 5.0 10 15.0 11 28.0 12 80.0 13 191.0 14 508.0 15 3584.0 16 7065.0 17 9104.0 18 13268.0 19 22076.0 20 37055.0 21 64350.0 22 106989.0 23 157466.0 24 162068.0 25 105506.0 26 69663.0 27 57095.0 28 49533.0 29 49770.0 30 57258.0 31 67318.0 32 83171.0 33 106619.0 34 143309.0 35 194355.0 36 311374.0 37 695232.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.094188934883114 15.66794535979095 10.720289833650607 22.517575871675326 2 14.530343210879934 23.438006267322358 37.42374667150482 24.607903850292892 3 20.68574474391992 33.431144059154185 25.663485778465507 20.219625418460392 4 12.883142897435787 18.042375176135884 34.5856479799783 34.48883394645003 5 13.345012594252918 39.37441744538119 29.841327565969756 17.439242394396132 6 28.270208573550846 34.084767386568124 20.777170349052234 16.867853690828795 7 26.48532122067274 34.56964417246556 21.24029158650783 17.70474302035387 8 29.466802587045503 28.165976186998265 21.90772073334846 20.459500492607777 9 32.337252957977604 14.521712037344953 20.57410561386388 32.56692939081356 10-11 24.08218780011002 26.872705955115194 25.95166927215259 23.093436972622197 12-13 27.04638127531025 24.98886970602038 28.8863762093754 19.07837280929397 14-15 25.686717771485934 23.085006697600985 30.039109483243564 21.189166047669513 16-17 20.117200247391665 27.218696873727687 29.926543944653883 22.73755893422676 18-19 20.57934276404287 25.164105029549884 35.11155140513687 19.145000801270378 20-21 22.672532149825063 24.598336002287454 33.96665633272495 18.762475515162535 22-23 21.021362031007133 26.793135184085177 31.90173240411134 20.283770380796355 24-25 20.038973617052356 27.932451946330474 32.53395720128094 19.494617235336236 26-27 20.88615930424729 27.25672470044887 32.62898705448058 19.228128940823257 28-29 20.37014734722166 27.922275195256045 32.988695661632846 18.718881795889445 30-31 21.464782045882952 28.083668864518955 32.10578905901568 18.34576003058242 32-33 18.759343231071895 28.83472620642286 32.62430964982891 19.78162091267634 34-35 18.185540640918457 29.950669293908135 32.24679261057258 19.61699745460083 36-37 19.548972516526447 30.28261622901755 30.884293115612095 19.284118138843912 38-39 19.433512713010128 29.543044129576046 30.852514475209553 20.170928682204273 40-41 18.517489700742665 30.40831446495921 30.79406069685296 20.280135137445164 42-43 19.48495544985218 29.908897231659402 30.546143262896813 20.060004055591605 44-45 19.55355683798448 29.392789229674605 30.535697903911064 20.51795602842985 46-47 18.505725519835515 28.997710181656323 31.446111350817507 21.050452947690655 48-49 18.530964814105914 29.280044572890212 30.871655206875293 21.317335406128578 50-51 17.563316325327836 28.744178724530123 30.856291283249437 22.836213666892604 52-53 18.013376606349006 27.66051995805175 30.46878515859467 23.857318277004577 54-55 18.445505158021774 27.03405077630439 30.57538061185482 23.945063453819017 56-57 19.087175816230285 25.722406008650424 30.686587950784684 24.503830224334614 58-59 18.466277785885143 25.76793368231159 31.433702866844605 24.332085664958665 60-61 19.11602993586841 25.364066982401805 31.040538739641644 24.47936434208814 62-63 18.182285784092052 25.573772344325945 31.78460212988939 24.459339741692606 64-65 17.907654894976467 25.488055698180712 31.68597870787781 24.918310698965012 66-67 18.389799526371505 25.34713398635239 31.49105281784011 24.772013669435996 68-69 18.837171097824555 24.97441782651204 31.543545818837092 24.644865256826314 70-71 18.30894101703477 25.13436577541608 31.429674456656354 25.1270187508928 72-73 19.089974171819275 24.793747760034314 31.090694468778068 25.02558359936834 74-75 19.094320647910575 24.456226959698533 30.88181439907386 25.56763799331703 76-77 18.76894108806399 24.74786952648717 30.182401545318005 26.300787840130834 78-79 19.137585965650768 24.692355512422512 29.519391356371838 26.650667165554886 80-81 18.97790757770905 24.778726145453792 28.515643769505637 27.72772250733152 82-83 18.90414666048884 24.65317581049305 27.528656891097075 28.914020637921034 84-85 19.511500961569244 24.251963155071486 26.910098936612947 29.32643694674632 86-87 19.685105288516237 23.809885288996288 26.49345499598688 30.0115544265006 88-89 19.955483500273456 23.984959314982095 25.898303724184746 30.1612534605597 90-91 20.702133384361787 24.235782537195565 25.316666197178993 29.745417881263652 92-93 20.52458959025126 24.900012442550413 24.4886889440163 30.086709023182028 94-95 20.52978267174115 24.99017600824846 24.916751017994756 29.56329030201563 96-97 21.01908427788673 25.146364337061822 24.520950593149486 29.313600791901962 98-99 21.58516426416685 25.452117378999283 24.235981748641734 28.726736608192134 100-101 21.786472399978866 25.496240175038924 24.30999947165097 28.407287953331238 102-103 21.68447384206093 26.372483737727542 24.53095426051337 27.412088159698158 104-105 21.67697594768723 26.410361701532526 24.98005093906271 26.932611411717538 106-107 21.057370158224998 26.91907246773186 25.522871297283356 26.500686076759788 108-109 20.989364644747432 27.38697215600593 25.609252479355536 26.014410719891096 110-111 20.73237722877824 27.507657176067653 26.208209922394847 25.551755672759256 112-113 19.962845189392382 27.865037155784062 26.389455385315674 25.782662269507885 114-115 19.920072904473262 27.62339124920538 26.969185714491477 25.487350131829878 116-117 19.579760315116513 28.068650216040247 27.38358047418893 24.968008994654316 118-119 19.253313449297167 28.750619511353182 27.54265263913296 24.45341440021669 120-121 19.023852594020195 29.305924852341825 27.76685887396826 23.90336367966972 122-123 18.567950515714248 30.114517340065934 27.57185375977878 23.745678384441035 124-125 17.84807013713446 31.3005454842829 27.941685511858193 22.909698866724447 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 48.0 1 29.0 2 22.5 3 61.0 4 106.5 5 232.0 6 580.0 7 1174.0 8 2115.5 9 2771.5 10 3014.5 11 3406.5 12 3874.5 13 4456.0 14 5109.5 15 5899.0 16 6833.5 17 7823.0 18 8794.0 19 9992.5 20 11532.0 21 13279.0 22 15399.0 23 17624.0 24 20229.5 25 23578.0 26 27561.0 27 32392.0 28 37486.5 29 42937.5 30 48629.0 31 53706.5 32 58403.5 33 60980.5 34 61220.0 35 59988.5 36 57879.0 37 55256.5 38 51553.5 39 47858.5 40 44528.5 41 42219.0 42 41337.0 43 43003.5 44 46793.5 45 51129.0 46 58945.5 47 67013.0 48 84673.5 49 104907.5 50 128255.5 51 142002.5 52 133856.0 53 123663.5 54 110453.5 55 96704.0 56 81916.5 57 66087.0 58 53668.5 59 43942.5 60 34115.5 61 26610.0 62 20862.0 63 14777.5 64 10005.0 65 7595.5 66 5839.5 67 4343.0 68 3504.5 69 2946.0 70 2524.5 71 2228.5 72 1930.0 73 1930.5 74 1573.5 75 1191.0 76 981.0 77 707.5 78 491.5 79 290.5 80 212.0 81 166.5 82 125.5 83 89.5 84 45.0 85 25.0 86 21.0 87 8.5 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12439511805492966 2 0.002719443555229568 3 0.0014374201649070572 4 0.0019036104886606974 5 0.010217337928933947 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.187100824535286E-4 20-21 5.438887110459135E-4 22-23 1.1654758093841003E-4 24-25 2.7194435552295676E-4 26-27 0.001476269358553194 28-29 0.0 30-31 0.001087777422091827 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0032633322662754807 42-43 0.28086024546474514 44-45 0.27060405834216505 46-47 0.28198687208048306 48-49 0.2903394487144025 50-51 0.29974095357676755 52-53 0.29371932856161637 54-55 0.30531581286498816 56-57 0.3038978172969042 58-59 0.3031208334239815 60-61 0.301858234630482 62-63 0.30195535761459735 64-65 0.31403745683854584 66-67 0.3236914814596108 68-69 0.32262312863434206 70-71 0.32600300848155594 72-73 0.3281785633257396 74-75 0.3332678076933835 76-77 0.3251871754149871 78-79 0.32442961613888743 80-81 0.5523966844544175 82-83 0.5160921129921028 84-85 0.4901991254269526 86-87 0.45976078220520455 88-89 0.3036647221350274 90-91 0.012761960112755898 92-93 0.08686679699276162 94-95 1.942459682306834E-5 96-97 0.0 98-99 3.884919364613668E-5 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.22853038162339903 114-115 0.2025985448646028 116-117 0.1932553137927069 118-119 0.17445230406797677 120-121 0.17901708432139782 122-123 0.1659054814658267 124-125 5.050395173997769E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2574056.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.69645493404568 #Duplication Level Percentage of deduplicated Percentage of total 1 70.3669933111263 25.11852205574914 2 15.42625474253087 11.013252144357235 3 6.311229673044548 6.758655768067396 4 2.7129647548993012 3.873728964436688 5 1.3666230240844055 2.439179859552911 6 0.7657673620963545 1.6401108078621336 7 0.5058658196193844 1.2640331502902207 8 0.3541950855991138 1.0114807128759375 9 0.24224647706065733 0.7782606406194379 >10 1.5807010688300427 10.773241936547134 >50 0.1845417234960156 4.573457436147745 >100 0.14992498598942655 10.85812016556132 >500 0.017056680847082343 4.193973508869907 >1k 0.014213900705901953 9.131618578546165 >5k 7.653638841639514E-4 1.8354098256785683 >10k+ 6.56026186426244E-4 4.7369544448381 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45219 1.7567216874846545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18028 0.7003732630525521 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17592 0.6834350146228365 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 16033 0.6228691217285094 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 14239 0.5531736683273402 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10257 0.3984761792284239 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7805 0.3032179564080968 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 7538 0.29284522170457833 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 7098 0.27575157650027815 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 6570 0.255239202255118 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6499 0.2524809095062423 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6378 0.24778015707505974 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 5138 0.19960715695385026 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4725 0.18356243997799582 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 4423 0.17182998349686254 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 4178 0.16231193105355904 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 4119 0.16001982862843697 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4109 0.1596313366919756 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4077 0.15838816249529925 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4048 0.1572615358795613 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4009 0.15574641732736197 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 3933 0.15279387861025556 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 3483 0.13531174146949404 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 3320 0.12897932290517378 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3126 0.12144257933782325 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3062 0.11895623094447053 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 3041 0.11814039787790165 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2910 0.11305115351025773 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 2785 0.10819500430449065 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 2756 0.1070683776887527 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 2647 0.10283381558132379 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 2638 0.10248417283850857 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 2587 0.10050286396255559 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.769838729227337E-5 0.0 3 0.0 0.0 0.0 7.769838729227337E-5 0.0 4 0.0 0.0 0.0 7.769838729227337E-5 0.0 5 0.0 0.0 0.0 1.1654758093841004E-4 0.0 6 0.0 0.0 0.0 1.1654758093841004E-4 0.0 7 0.0 0.0 0.0 1.1654758093841004E-4 0.0 8 0.0 0.0 0.0 1.5539677458454673E-4 0.0 9 0.0 0.0 0.0 1.5539677458454673E-4 0.0 10-11 0.0 0.0 0.0 4.079165332844352E-4 1.942459682306834E-5 12-13 0.0 0.0 0.0 8.352576633919387E-4 3.884919364613668E-5 14-15 0.0 0.0 0.0 0.0011654758093841006 3.884919364613668E-5 16-17 0.0 0.0 0.0 0.002350376215591269 3.884919364613668E-5 18-19 0.0 0.0 0.0 0.00456478025342106 3.884919364613668E-5 20-21 0.0 0.0 0.0 0.009498627846480418 3.884919364613668E-5 22-23 0.0 0.0 0.0 0.026611697647603627 3.884919364613668E-5 24-25 0.0 0.0 0.0 0.06839400541402363 3.884919364613668E-5 26-27 0.0 0.0 0.0 0.1278138470957897 3.884919364613668E-5 28-29 0.0 0.0 0.0 0.26462128252066 3.884919364613668E-5 30-31 0.0 0.0 0.0 0.5748320937850614 3.884919364613668E-5 32-33 0.0 0.0 0.0 1.1149524330472997 3.884919364613668E-5 34-35 0.0 0.0 0.0 1.5488590768810004 3.884919364613668E-5 36-37 0.0 0.0 0.0 2.0988859605230035 3.884919364613668E-5 38-39 0.0 0.0 0.0 2.747434399251609 3.884919364613668E-5 40-41 0.0 0.0 0.0 3.716877177497304 3.884919364613668E-5 42-43 0.0 0.0 0.0 4.542364268687239 3.884919364613668E-5 44-45 0.0 0.0 0.0 5.241882849479577 3.884919364613668E-5 46-47 0.0 0.0 0.0 5.930581929841464 3.884919364613668E-5 48-49 0.0 0.0 0.0 6.548730874541969 3.884919364613668E-5 50-51 0.0 0.0 0.0 7.159925813579814 3.884919364613668E-5 52-53 0.0 0.0 0.0 7.865310622612717 5.827379046920503E-5 54-55 0.0 0.0 0.0 8.505817278256572 7.769838729227337E-5 56-57 0.0 0.0 0.0 9.111320810425259 7.769838729227337E-5 58-59 0.0 0.0 0.0 9.774165752415643 7.769838729227337E-5 60-61 0.0 0.0 0.0 10.388507476138827 7.769838729227337E-5 62-63 0.0 0.0 0.0 10.998944855900572 7.769838729227337E-5 64-65 0.0 0.0 0.0 11.627233440142717 7.769838729227337E-5 66-67 0.0 0.0 0.0 12.22683189487719 7.769838729227337E-5 68-69 0.0 0.0 0.0 12.879051582405356 7.769838729227337E-5 70-71 0.0 0.0 0.0 13.61534092498376 7.769838729227337E-5 72-73 0.0 0.0 0.0 14.254138216107187 7.769838729227337E-5 74-75 0.0 0.0 0.0 14.854066888987653 7.769838729227337E-5 76-77 0.0 0.0 0.0 15.458929409461177 9.71229841153417E-5 78-79 0.0 0.0 0.0 16.05079687466007 1.1654758093841004E-4 80-81 0.0 0.0 0.0 16.660204750790193 1.1654758093841004E-4 82-83 0.0 0.0 0.0 17.366016900953205 1.1654758093841004E-4 84-85 0.0 0.0 0.0 17.964449879878295 1.1654758093841004E-4 86-87 0.0 0.0 0.0 18.54380790472313 1.1654758093841004E-4 88-89 0.0 0.0 0.0 19.129071007002178 1.1654758093841004E-4 90-91 0.0 0.0 0.0 19.704602386272867 1.1654758093841004E-4 92-93 0.0 0.0 0.0 20.248005482398206 1.1654758093841004E-4 94-95 0.0 0.0 0.0 20.810600080184734 1.1654758093841004E-4 96-97 0.0 0.0 0.0 21.364201089642183 1.1654758093841004E-4 98-99 0.0 0.0 0.0 21.92988419832358 1.1654758093841004E-4 100-101 0.0 0.0 0.0 22.54395397769124 1.1654758093841004E-4 102-103 0.0 0.0 0.0 23.09081465205108 1.1654758093841004E-4 104-105 0.0 0.0 0.0 23.623165152584093 1.1654758093841004E-4 106-107 0.0 0.0 0.0 24.138616253881032 1.1654758093841004E-4 108-109 0.0 0.0 0.0 24.679202783467026 1.1654758093841004E-4 110-111 0.0 0.0 0.0 25.206658285600625 1.1654758093841004E-4 112-113 1.942459682306834E-5 0.0 0.0 25.7554808442396 1.942459682306834E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 905 0.0 73.5655 1 TACAACG 545 0.0 61.01314 2 GTATAAC 825 0.0 57.642227 1 GACCGTT 425 0.0 55.86645 7 ATAGTAC 1290 0.0 52.931564 4 TAGTACT 1365 0.0 52.198162 5 AGATTAC 1515 0.0 51.344097 2 TAACGCA 1045 0.0 49.43215 4 ATAACGC 1075 0.0 49.158268 3 GCATAGT 1385 0.0 48.875023 2 AGTGTAC 1345 0.0 47.67783 3 GATTACT 1720 0.0 45.568054 3 CATAGTA 1515 0.0 45.071312 3 GTACAAC 780 0.0 44.96371 1 GTGTACT 1505 0.0 44.580868 4 TCACGCA 630 0.0 42.411003 4 TATCACG 605 0.0 42.20316 2 GGTTTCA 1395 0.0 40.055153 1 TTAGGAC 700 0.0 39.867115 3 ACAACGC 855 0.0 39.58437 3 >>END_MODULE