FastQCFastQC Report
Fri 10 Feb 2017
SRR3558074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413954
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288491.1950931956449873No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA134830.5585441976110563No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT115970.48041511975787443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114790.47552687416578776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113160.46877446711909176No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC68140.28227547003795433No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT64020.2652080362757534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62760.2599883842028473No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA62010.2568814484451651No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG57740.2391926275314277No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG56950.23591998853333576No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38410.15911653660343156No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC36250.1501685616213068No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG36220.1500442841909995No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32860.1361252119965832No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32520.1347167344531006No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA31560.13073985668326737No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA29760.12328321086483007No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG28590.11843639108284582No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC25610.10609149967232183No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25110.10402020916720035No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC24950.10335739620556149No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT24540.10165893799136189No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24510.10153466056105459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6150.067.6979141
TATCACG4600.040.028662
TAGTACT11150.038.886355
GCATAGT10800.038.498292
TACAACG5450.037.0552372
ATAGTAC11450.036.8307884
GACCGTT3100.036.3995557
ATCACGC5300.035.8618163
GTATAAC6850.034.731361
AGATTAC10450.034.6721422
ATCTACG6400.033.411022
TCACGCA5750.033.055244
TACGCAG6150.032.8362055
CATGGGT34850.032.7232634
TAACGCA7150.032.3978924
ACAACGC6300.032.0550543
CATGGGG76900.031.1269724
GGTTTCA13750.029.8468671
GATTACT12600.029.6980653
GTACAAC7100.029.3198721
Produced by FastQC (version 0.11.5)