FastQCFastQC Report
Fri 10 Feb 2017
SRR3558073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213236
Sequences flagged as poor quality0
Sequence length125
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT658082.9733837692862397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266591.2045258616794594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257351.1627770377853965No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA196420.8874787867177292No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT192730.8708063667860092No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142530.6439891633788715No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA98930.4469925484674928No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG95090.4296423878881421No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA90100.4070962156769544No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC87650.3960264517656499No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG78260.35359988722395624No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT73000.32983378184703305No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA69310.3131613619153132No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA62230.28117200334713516No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC61700.278777319725506No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC61330.2771055594613498No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC61310.2770151940416657No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG60760.27453014500035244No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59860.2704637011145671No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA55830.2522550690482172No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC55610.25126104943169186No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51590.233097600075184No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.2298896276763978No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA50000.22591354921029658No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC48730.22017534506035505No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA41060.18552020661149557No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT40320.1821766860831832No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA37780.1707002777833001No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT37590.16984180629630097No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT36470.16478134279399032No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.1540730405614223No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC33620.15190427048900343No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG32920.14874148080005928No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA32610.14734081679495545No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC32380.14630161446858808No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT32320.14603051820953572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC31770.14354546916822247No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.14006640051038388No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30860.13943384257259506No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA29840.134825206168705No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT29630.13387636926202176No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT28920.13066839686323553No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA27290.12330361515897988No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA27290.12330361515897988No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA26780.12099929695703485No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG26600.12018600817987779No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA26400.11928235398303659No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG26210.11842388249603747No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26160.11819796894682719No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGGTGGAAAA25960.11729431474998599No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25660.11593883345472421No Hit
GGTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAA24450.11047172556383504No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT24410.1102909947244668No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG24350.11001989846541443No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA24210.10938734052762561No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA24190.10929697510794148No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC24120.10898069613904707No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23950.10821259007173208No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA23550.1064052816780497No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC23140.10455279057452528No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC23120.10446242515484115No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT23070.10423651160563084No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG22700.10256475134147465No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA22630.10224847237258024No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA22580.10202255882336994No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG22540.10184182798400171No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG22510.10170627985447553No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA22490.1016159144347914No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA22300.10075744294779229No Hit
GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA22270.1006218948182661No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG22250.100531529398582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC5300.080.663883
CGCATAG8300.080.207331
TACAACG5700.078.1301652
TTAGGAC3850.077.113743
TAGGACC3850.077.110254
GTACAAC5950.074.923561
GTATAAC5950.071.926621
GACCGTT4150.071.527937
CATAGTA9700.069.783963
ATAGTAC9850.069.3209464
AGTGTAC10350.068.843573
GGTTTCA11350.067.5566251
CTTAGGA4400.067.476042
GCATAGT10050.067.3551942
AGATTAC13350.065.8283162
TAGTACT11250.062.277675
TCACGCA5150.062.257174
GTGTACT12000.059.3748974
TGAGTAC3150.058.4324385
GCAAGTA1850.054.564412
Produced by FastQC (version 0.11.5)