##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3558069.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1781450 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.647196946307783 33.0 32.0 34.0 27.0 34.0 2 31.9652838979483 33.0 32.0 34.0 27.0 34.0 3 32.113159504897695 33.0 32.0 34.0 28.0 34.0 4 31.970096269892505 33.0 32.0 34.0 28.0 34.0 5 32.135778158241884 33.0 32.0 34.0 30.0 34.0 6 35.48633135928598 38.0 36.0 38.0 29.0 38.0 7 35.793838165539306 38.0 37.0 38.0 31.0 38.0 8 35.976876701563334 38.0 37.0 38.0 31.0 38.0 9 36.10129781919223 38.0 37.0 38.0 33.0 38.0 10-11 36.111025288388674 38.0 37.0 38.0 32.5 38.0 12-13 36.08441690757529 38.0 37.0 38.0 32.0 38.0 14-15 36.08852199051334 38.0 37.0 38.0 32.5 38.0 16-17 36.02049145359118 38.0 37.0 38.0 31.0 38.0 18-19 35.991210811417666 38.0 37.0 38.0 31.0 38.0 20-21 35.959626147239604 38.0 37.0 38.0 31.0 38.0 22-23 35.906918240758934 38.0 37.0 38.0 31.0 38.0 24-25 35.82053467680822 38.0 37.0 38.0 31.0 38.0 26-27 35.71147520278425 38.0 37.0 38.0 29.0 38.0 28-29 35.630699991579895 38.0 37.0 38.0 29.0 38.0 30-31 35.53046478991833 38.0 37.0 38.0 29.0 38.0 32-33 35.428547250834995 38.0 37.0 38.0 28.5 38.0 34-35 35.250151000589405 38.0 36.0 38.0 27.5 38.0 36-37 35.10961744646215 38.0 36.0 38.0 27.0 38.0 38-39 34.959057790002525 38.0 36.0 38.0 25.5 38.0 40-41 34.75882062364927 38.0 36.0 38.0 25.0 38.0 42-43 34.39098823991692 38.0 35.0 38.0 20.0 38.0 44-45 34.129913834236156 38.0 35.0 38.0 16.0 38.0 46-47 33.7202430604283 38.0 34.0 38.0 15.5 38.0 48-49 33.364447219961264 38.0 34.0 38.0 15.0 38.0 50-51 33.07316455696203 38.0 33.0 38.0 15.0 38.0 52-53 32.822659350529065 38.0 31.5 38.0 15.0 38.0 54-55 32.66531252631283 38.0 31.0 38.0 15.0 38.0 56-57 32.50145078447332 38.0 29.0 38.0 15.0 38.0 58-59 32.385977153442425 38.0 29.0 38.0 15.0 38.0 60-61 32.25041454994527 38.0 28.5 38.0 15.0 38.0 62-63 32.15846389177355 38.0 27.5 38.0 15.0 38.0 64-65 32.07992281568386 38.0 27.0 38.0 15.0 38.0 66-67 31.926860142019144 38.0 26.5 38.0 15.0 38.0 68-69 31.83567739762553 38.0 26.0 38.0 15.0 38.0 70-71 31.654782620898708 38.0 26.0 38.0 15.0 38.0 72-73 31.509650846220772 38.0 25.5 38.0 14.0 38.0 74-75 31.39328243846305 38.0 25.0 38.0 14.0 38.0 76-77 31.316810463386567 38.0 25.0 38.0 14.0 38.0 78-79 31.112282129725784 38.0 25.0 38.0 14.0 38.0 80-81 31.04092424710208 38.0 24.5 38.0 14.0 38.0 82-83 30.932256027393414 38.0 24.0 38.0 14.0 38.0 84-85 30.835445564006847 38.0 23.0 38.0 14.0 38.0 86-87 30.729808582895956 38.0 23.0 38.0 14.0 38.0 88-89 30.618563810379186 37.5 23.0 38.0 13.5 38.0 90-91 30.62227483229953 37.0 23.0 38.0 13.0 38.0 92-93 30.585183418002188 37.0 23.0 38.0 13.0 38.0 94-95 30.52143338291841 37.0 23.0 38.0 13.0 38.0 96-97 30.458594122765163 37.0 21.0 38.0 13.0 38.0 98-99 30.38000533273457 37.0 21.0 38.0 13.0 38.0 100-101 30.309084734345618 37.0 21.0 38.0 13.0 38.0 102-103 30.23181565578602 37.0 21.0 38.0 13.0 38.0 104-105 30.06104577731623 37.0 21.0 38.0 13.0 38.0 106-107 29.939209351932416 36.0 21.0 38.0 13.0 38.0 108-109 29.837178422071908 36.0 21.0 38.0 13.0 38.0 110-111 29.733679306183166 36.0 18.0 38.0 13.0 38.0 112-113 29.408395127564624 35.5 15.0 38.0 13.0 38.0 114-115 29.287656964832017 35.0 15.0 38.0 13.0 38.0 116-117 29.175821381458924 35.0 15.0 38.0 13.0 38.0 118-119 28.979576468607036 35.0 15.0 38.0 13.0 38.0 120-121 28.825195767492772 35.0 15.0 38.0 13.0 38.0 122-123 28.612926829268293 34.0 14.0 38.0 13.0 38.0 124-125 27.345673748912404 31.0 14.0 37.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3166718109426512 1101 2 -0.6106701838433928 1101 3 -0.2616343984933742 1101 4 -0.3368531443155689 1101 5 -0.31301351539266165 1101 6 -0.0400224541677332 1101 7 0.01908897230576656 1101 8 -0.026419362469106034 1101 9 -0.07218742997637406 1101 10-11 0.06384207749854198 1101 12-13 0.03397921419944794 1101 14-15 0.185330614674605 1101 16-17 0.13016566490527737 1101 18-19 0.04055712929177702 1101 20-21 0.04982691449200871 1101 22-23 0.08313919376585233 1101 24-25 0.18926514501508507 1101 26-27 0.04573762104707413 1101 28-29 0.15915438419340688 1101 30-31 0.2060447377990684 1101 32-33 0.02601349178454626 1101 34-35 -0.056145262431819276 1101 36-37 0.1647180891158868 1101 38-39 0.07037091381658911 1101 40-41 -0.08263590117293518 1101 42-43 -0.20548013033711499 1101 44-45 0.03600763329861678 1101 46-47 -0.07291552251592037 1101 48-49 -0.09522074858865182 1101 50-51 -0.11800453050724258 1101 52-53 -0.2053394797222694 1101 54-55 0.03132436525491755 1101 56-57 -0.05866665368851898 1101 58-59 -0.026041829551246565 1101 60-61 -0.15894549709754102 1101 62-63 0.06753126433920897 1101 64-65 -0.043347022166393856 1101 66-67 -0.107991982387869 1101 68-69 0.004758822039953259 1101 70-71 0.025792580300674217 1101 72-73 0.1525772244612824 1101 74-75 -0.0558249948386802 1101 76-77 -0.12728488974403973 1101 78-79 0.00978943947106714 1101 80-81 -0.1281531180776163 1101 82-83 -0.1382056652399406 1101 84-85 -0.1345031384021418 1101 86-87 -0.07192741382583634 1101 88-89 -0.11849865976936869 1101 90-91 0.05558485050805828 1101 92-93 0.19065225721809043 1101 94-95 0.14313787413418666 1101 96-97 0.13487219992455834 1101 98-99 -0.008124322469317491 1101 100-101 -0.004335068204429859 1101 102-103 0.10492011778379506 1101 104-105 -0.032160460621806664 1101 106-107 0.2061188080230849 1101 108-109 0.09161116707104 1101 110-111 -0.14117350877360124 1101 112-113 -0.27157354342742224 1101 114-115 -0.24389499829203132 1101 116-117 -0.12031617013570539 1101 118-119 -0.027841124761124547 1101 120-121 0.1979288460080042 1101 122-123 -0.465929747947353 1101 124-125 -0.42332736651334457 1102 1 -0.5081050281259465 1102 2 -0.4188620314966798 1102 3 -0.17506517179876724 1102 4 -0.2610042140463875 1102 5 0.05749041948388367 1102 6 0.3560732339483792 1102 7 0.17643287721340073 1102 8 0.33882932833371626 1102 9 0.07589900844391906 1102 10-11 0.14739158674790787 1102 12-13 0.10950355681636381 1102 14-15 0.13276052802508076 1102 16-17 0.26126475593034115 1102 18-19 0.18678686158547464 1102 20-21 0.14630181164385903 1102 22-23 0.021923419550638812 1102 24-25 -0.023154889862311734 1102 26-27 0.07823766799491239 1102 28-29 0.03626112804015946 1102 30-31 -0.027352887093520906 1102 32-33 0.04432573960179553 1102 34-35 -0.06384114662800755 1102 36-37 0.038541560057119284 1102 38-39 0.0349622053484282 1102 40-41 0.08654339230416497 1102 42-43 -0.385636314468492 1102 44-45 -0.1293855597178748 1102 46-47 -0.16744321753238722 1102 48-49 -0.20876086179446673 1102 50-51 -0.061777692790720096 1102 52-53 -0.16346330157431765 1102 54-55 -0.00994084341689927 1102 56-57 0.0834077090997809 1102 58-59 0.017188227168219328 1102 60-61 -0.024681797879722467 1102 62-63 -0.025952084071391823 1102 64-65 -0.04507162678464027 1102 66-67 0.0425969377923856 1102 68-69 0.024224205940537757 1102 70-71 -0.09442076363531626 1102 72-73 -0.05268342670849435 1102 74-75 -0.14818256642442407 1102 76-77 -0.4400152984104153 1102 78-79 -0.14095958903241979 1102 80-81 -0.41931407016685895 1102 82-83 -0.3086818523241668 1102 84-85 -0.485126767606161 1102 86-87 -0.45908569021198176 1102 88-89 -0.14294197170959322 1102 90-91 -0.3202714619042375 1102 92-93 -0.3397186242421739 1102 94-95 -0.35923622956679324 1102 96-97 -0.07523184943995886 1102 98-99 -0.24144618326152312 1102 100-101 -0.338285900541063 1102 102-103 -0.2944182090728944 1102 104-105 -0.39347378921071297 1102 106-107 -0.3029848638160466 1102 108-109 -0.3711098196495186 1102 110-111 -0.5384273515352476 1102 112-113 -0.8313363629398509 1102 114-115 -0.5738123636181065 1102 116-117 -0.14922373599057792 1102 118-119 -0.20984802512226608 1102 120-121 -0.1612792766806237 1102 122-123 -0.1612374570417039 1102 124-125 -0.3228769586499354 1103 1 -0.6229773760065704 1103 2 -0.5309824630355635 1103 3 -0.34778376115365006 1103 4 -0.36290383629787115 1103 5 -0.3432151590665278 1103 6 -0.23572861527540567 1103 7 -0.19937393825941285 1103 8 -0.141551588086422 1103 9 -0.2552074059210838 1103 10-11 -0.25536368949169486 1103 12-13 -0.2680929642493979 1103 14-15 -0.329271986186491 1103 16-17 -0.2291761746653762 1103 18-19 -0.2170322223143728 1103 20-21 -0.21812646973638294 1103 22-23 -0.2573248913007049 1103 24-25 -0.32890335510896307 1103 26-27 -0.2507211316575777 1103 28-29 -0.2743123105273071 1103 30-31 -0.14597264105299956 1103 32-33 -0.14193643802800437 1103 34-35 -0.1468877420115149 1103 36-37 -0.13729693445264957 1103 38-39 -0.08741455439807311 1103 40-41 -0.09612225466017321 1103 42-43 -0.21796109179526013 1103 44-45 -0.15770485881118645 1103 46-47 -0.16501433137052857 1103 48-49 -0.2348402289817031 1103 50-51 -0.29180733988944496 1103 52-53 -0.40672438643733955 1103 54-55 -0.39899221625516645 1103 56-57 -0.40553726697294934 1103 58-59 -0.3052438908953974 1103 60-61 -0.2780273761741441 1103 62-63 -0.5158173521672431 1103 64-65 -0.22382219276743598 1103 66-67 -0.36535978032338434 1103 68-69 -0.4393143424875916 1103 70-71 -0.3161653825988999 1103 72-73 -0.6083996529352191 1103 74-75 -0.5780046998315669 1103 76-77 -0.6113190507763449 1103 78-79 -0.5281351788944875 1103 80-81 -0.5212714506624927 1103 82-83 -0.4753495856250751 1103 84-85 -0.6090764395361319 1103 86-87 -0.413517795475979 1103 88-89 -0.5003730258055477 1103 90-91 -0.6320291844694701 1103 92-93 -0.40032622177120913 1103 94-95 -0.4690711305858031 1103 96-97 -0.378521669429432 1103 98-99 -0.548134779928187 1103 100-101 -0.5538737466705435 1103 102-103 -0.7122621466171957 1103 104-105 -0.7427289318543764 1103 106-107 -0.4941371327870705 1103 108-109 -0.554644560193406 1103 110-111 -0.7506527398834422 1103 112-113 -0.8635041850380567 1103 114-115 -0.8711321250973576 1103 116-117 -0.7029043441000873 1103 118-119 -0.6502205577972724 1103 120-121 -0.45040392729124434 1103 122-123 -0.6039284792346287 1103 124-125 -0.8456210672290361 1104 1 -0.23216895200867071 1104 2 -0.09010658709716779 1104 3 -0.06566976737421726 1104 4 -0.3492279553630375 1104 5 -0.18251809322593715 1104 6 0.15368548967481388 1104 7 0.10756299417622728 1104 8 0.23626952686917235 1104 9 0.16702331718870056 1104 10-11 0.24705146471093542 1104 12-13 -0.013418170040267796 1104 14-15 0.0537611964209006 1104 16-17 0.08928145552312117 1104 18-19 0.0998746377977966 1104 20-21 -0.017711609074602563 1104 22-23 0.07903310987345691 1104 24-25 -0.08178505189999896 1104 26-27 0.02054184347264254 1104 28-29 0.09054913194493253 1104 30-31 0.11225571499331721 1104 32-33 0.0910305417488999 1104 34-35 0.10901789397284745 1104 36-37 0.05811789498137898 1104 38-39 0.06806872626444971 1104 40-41 0.05243830219306744 1104 42-43 -0.12202756942993886 1104 44-45 0.01665200567287428 1104 46-47 -0.07562942436677389 1104 48-49 -0.27048686200567573 1104 50-51 -0.3467110335077379 1104 52-53 -0.23963531240259073 1104 54-55 -0.3576138858156028 1104 56-57 -0.3769676228443828 1104 58-59 -0.4009931695343596 1104 60-61 -0.2821709863518933 1104 62-63 -0.21368824415669962 1104 64-65 -0.18765967730969635 1104 66-67 -0.22967551786038953 1104 68-69 -0.29409109530121214 1104 70-71 -0.31253609346740774 1104 72-73 -0.4168770502896031 1104 74-75 -0.26111876231205144 1104 76-77 -0.3965277146033692 1104 78-79 -0.0665704500138169 1104 80-81 -0.28124606250155537 1104 82-83 -0.33800369457244983 1104 84-85 -0.48194577013807915 1104 86-87 -0.361726884000408 1104 88-89 -0.17314164691301315 1104 90-91 -0.26002939057262253 1104 92-93 -0.3607854882938142 1104 94-95 -0.21259433904977243 1104 96-97 -0.26898003034727935 1104 98-99 -0.2425543809624493 1104 100-101 -0.39478241174667517 1104 102-103 -0.3139465714644878 1104 104-105 -0.4632063729276119 1104 106-107 -0.4891778537733664 1104 108-109 -0.41543000056714163 1104 110-111 -0.23995239512907673 1104 112-113 -0.5973570960889347 1104 114-115 -0.33564725181956945 1104 116-117 -0.3783627674734369 1104 118-119 -0.3558282810444595 1104 120-121 -0.2902350788457646 1104 122-123 -0.4401737482304675 1104 124-125 -0.2423048362935738 1105 1 0.03196435678851017 1105 2 0.07947374782132854 1105 3 0.03452490763474003 1105 4 0.12243520844484834 1105 5 0.03583632182893126 1105 6 0.19026090444881305 1105 7 -0.014208160992339458 1105 8 0.14904955379437013 1105 9 0.04069884915511324 1105 10-11 -0.0017112351699353212 1105 12-13 0.06435498030501208 1105 14-15 -0.04318707910719155 1105 16-17 0.03744862905506352 1105 18-19 -0.07736134027462782 1105 20-21 0.03772536852324748 1105 22-23 0.12680173232984515 1105 24-25 0.0011346373948128985 1105 26-27 0.08347828837767679 1105 28-29 0.12024736146773307 1105 30-31 0.01970941914541413 1105 32-33 0.1684677324238706 1105 34-35 0.14287112741821062 1105 36-37 0.09243783996873844 1105 38-39 0.08017901304689445 1105 40-41 0.06535791884795117 1105 42-43 0.04228394932568591 1105 44-45 0.012013340891613211 1105 46-47 0.05372352336153341 1105 48-49 0.016354293840869616 1105 50-51 0.005539351483882626 1105 52-53 -0.10989313572290627 1105 54-55 -0.2081753924645824 1105 56-57 -0.10884032863329907 1105 58-59 -0.16946671407232827 1105 60-61 -0.06904664616457978 1105 62-63 -0.261640282433099 1105 64-65 -0.08575493731221684 1105 66-67 -0.0013779072297772643 1105 68-69 -0.12486124803876564 1105 70-71 -0.056448867441005746 1105 72-73 -0.08633752932572136 1105 74-75 -0.2633324641396726 1105 76-77 -0.289956464518621 1105 78-79 -0.17032024420068836 1105 80-81 -0.2988436905657501 1105 82-83 -0.3004246381166986 1105 84-85 -0.31745870488805394 1105 86-87 -0.19920472882214924 1105 88-89 -0.12517689664908715 1105 90-91 -0.1583110930588454 1105 92-93 -0.20814175808818902 1105 94-95 -0.3218647678190578 1105 96-97 -0.28337000022592207 1105 98-99 -0.18525237319481747 1105 100-101 -0.2907027112168876 1105 102-103 -0.16582165015774564 1105 104-105 -0.25834300863352766 1105 106-107 -0.24204107763025817 1105 108-109 -0.1266281719780018 1105 110-111 -0.15312165100565167 1105 112-113 -0.29551700562800676 1105 114-115 -0.28652510535810194 1105 116-117 -0.10696304447369798 1105 118-119 -0.13702410376429341 1105 120-121 -0.09100130902716685 1105 122-123 -0.2451954675691539 1105 124-125 -0.12669415241285975 1106 1 0.27973238384713284 1106 2 0.10364086232171488 1106 3 0.09414227396347741 1106 4 0.05811075945074151 1106 5 -0.04320358327955631 1106 6 0.4454073943603376 1106 7 0.2344168096096908 1106 8 -0.03847110999176806 1106 9 0.15287835454218168 1106 10-11 4.4830800559481077E-4 1106 12-13 0.1221312103896679 1106 14-15 0.2831517638116736 1106 16-17 0.2596999336429633 1106 18-19 0.12195898448339193 1106 20-21 0.08751401562610539 1106 22-23 0.14138184419548594 1106 24-25 0.04135346617993463 1106 26-27 0.025955562473498617 1106 28-29 0.08012993435480098 1106 30-31 -0.0014561206466510157 1106 32-33 0.027185663079897893 1106 34-35 0.13376457556361032 1106 36-37 0.07267743763417656 1106 38-39 0.17670431574650536 1106 40-41 0.22278188463610604 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96-97 0.32027763153041633 2314 98-99 0.3560182105088039 2314 100-101 0.4334894171071113 2314 102-103 0.522998721919496 2314 104-105 0.4376670430464138 2314 106-107 0.4646104794171535 2314 108-109 0.44847110670390933 2314 110-111 0.5069491739016065 2314 112-113 0.6349145471089166 2314 114-115 0.6119721868330465 2314 116-117 0.33488211746748675 2314 118-119 0.3270315106400581 2314 120-121 0.7039114036882985 2314 122-123 0.9562760580209009 2314 124-125 0.5935973325485975 2315 1 0.4311656954066976 2315 2 0.4029304207380484 2315 3 0.16109315580126093 2315 4 0.14613990547567823 2315 5 0.13000716178810734 2315 6 -0.3984593385910884 2315 7 -0.4185740974317156 2315 8 -0.5868973286967858 2315 9 -0.31017694891291114 2315 10-11 -0.42907577128176655 2315 12-13 -0.33599984845155006 2315 14-15 -0.09681843695021541 2315 16-17 -0.2808849811791987 2315 18-19 -0.30577078180502326 2315 20-21 -0.07212261286901622 2315 22-23 -0.27438617263775456 2315 24-25 -0.19246361100904608 2315 26-27 -0.43188140592369706 2315 28-29 -0.3360665729779484 2315 30-31 -0.19349934832901994 2315 32-33 -0.1543403989695733 2315 34-35 -0.2572469510476054 2315 36-37 -0.2071521488761121 2315 38-39 -0.22687780702288762 2315 40-41 -0.44638703166589266 2315 42-43 -0.11534546229940901 2315 44-45 -0.267036335134307 2315 46-47 -0.35487781050977674 2315 48-49 -0.39154153603119823 2315 50-51 -0.5079809295309943 2315 52-53 -0.5624522936276435 2315 54-55 -0.5217431422094307 2315 56-57 -0.45180130933726304 2315 58-59 -0.1973059374839039 2315 60-61 -0.2684716811727661 2315 62-63 -0.2536774029799389 2315 64-65 -0.25960382061034437 2315 66-67 -0.0929830623338539 2315 68-69 -0.30182467338232044 2315 70-71 -0.1356142484962355 2315 72-73 -0.32790055593194367 2315 74-75 -0.424463881318605 2315 76-77 -0.4882268226701889 2315 78-79 -0.29605660287440827 2315 80-81 -0.3709783593526872 2315 82-83 -0.39371255969835417 2315 84-85 -0.4844774313020288 2315 86-87 -0.3295704753865998 2315 88-89 -0.34180756619552355 2315 90-91 -0.526369384659251 2315 92-93 -0.27705033331426066 2315 94-95 -0.4488845622794564 2315 96-97 -0.362365680571493 2315 98-99 -0.1961060983034031 2315 100-101 -0.39228401328415075 2315 102-103 -0.2486444968574837 2315 104-105 -0.3167402269659476 2315 106-107 -0.4968935685518616 2315 108-109 -0.480192480239932 2315 110-111 -0.3251400214041489 2315 112-113 -0.18696373384579346 2315 114-115 -0.10947504958234688 2315 116-117 -0.2448401241974274 2315 118-119 -0.20476079471912811 2315 120-121 -0.37787798526669647 2315 122-123 -0.11391654131182705 2315 124-125 -0.08330357970263336 2316 1 -0.2768590969540128 2316 2 -0.01240445284578584 2316 3 0.06462613265168926 2316 4 0.23951868274367172 2316 5 0.16457051386188226 2316 6 -0.08407381345511311 2316 7 -0.32841413827235044 2316 8 -0.32424622161447303 2316 9 -0.25720454906736734 2316 10-11 -0.4416866267752937 2316 12-13 -0.3793377196333694 2316 14-15 -0.05717176736012419 2316 16-17 -0.16098646099776204 2316 18-19 -0.13235122935032706 2316 20-21 -0.1968122174871496 2316 22-23 -0.06585430843286844 2316 24-25 -0.24729903764316674 2316 26-27 -0.2179502655929184 2316 28-29 -0.14401904337830018 2316 30-31 0.1009442961741911 2316 32-33 -0.1101740977306136 2316 34-35 -0.13204471135757245 2316 36-37 -0.18673510314638264 2316 38-39 -0.29084559920688235 2316 40-41 -0.15677979554287447 2316 42-43 -0.05813290118570791 2316 44-45 -0.2370482469865962 2316 46-47 -0.2523406514211004 2316 48-49 -0.3680557157169133 2316 50-51 -0.2833602107619768 2316 52-53 -0.4151394962403927 2316 54-55 -0.5010011385311586 2316 56-57 -0.405114047819751 2316 58-59 -0.3185222881374159 2316 60-61 -0.38591060020489465 2316 62-63 -0.2890153527014512 2316 64-65 -0.45590754666392286 2316 66-67 -0.34545918954196253 2316 68-69 -0.5259687544352758 2316 70-71 -0.31250738216423457 2316 72-73 -0.357928404795409 2316 74-75 -0.2465225700985556 2316 76-77 -0.015684296703007305 2316 78-79 -0.14735921628244597 2316 80-81 -0.2394579292170107 2316 82-83 -0.3639787437822868 2316 84-85 -0.2878934167460052 2316 86-87 -0.49731668093804515 2316 88-89 -0.3881000021693133 2316 90-91 -0.5452012377376825 2316 92-93 -0.40329240571476177 2316 94-95 -0.5215735320351342 2316 96-97 -0.37987610344224976 2316 98-99 -0.6052829696750344 2316 100-101 -0.4596349024188413 2316 102-103 -0.4162516217159471 2316 104-105 -0.7960877258042167 2316 106-107 -0.7046091109011172 2316 108-109 -0.7114956172458697 2316 110-111 -0.6549587583551073 2316 112-113 -0.6668209225177577 2316 114-115 -0.7416512860915141 2316 116-117 -0.6951382477534445 2316 118-119 -0.9404520094008113 2316 120-121 -0.7279781233656415 2316 122-123 -0.8557526682581376 2316 124-125 -0.942368368398153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 15.0 11 21.0 12 42.0 13 127.0 14 320.0 15 2384.0 16 4629.0 17 6016.0 18 8804.0 19 13751.0 20 22132.0 21 37168.0 22 57550.0 23 81890.0 24 87931.0 25 62438.0 26 41744.0 27 36225.0 28 34347.0 29 36175.0 30 41978.0 31 49878.0 32 61425.0 33 78731.0 34 105358.0 35 147622.0 36 239856.0 37 522888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.51042026715405 15.055025282798088 9.92118691173305 23.513367538314807 2 14.617589024332053 22.751158502185085 37.8281627600841 24.80308971339877 3 20.509602122790188 32.12061777370864 25.814500605974196 21.555279497526985 4 12.573894660498558 17.748920665019664 35.13341315724918 34.54377151723259 5 13.232828940055533 39.38034516713243 31.177938136690315 16.208887756121715 6 28.29745432091835 33.98321591961604 21.249094838474278 16.470234920991327 7 27.209015128125962 33.74874400067361 21.425748687866626 17.6164921833338 8 28.65946279715962 29.784276853125263 22.094192932723345 19.462067416991776 9 31.554969266608662 14.64267871677566 20.213477784950463 33.588874231665216 10-11 24.588537427376576 26.361110331471554 26.822279603693623 22.22807263745825 12-13 26.81307361980409 24.497488001347218 28.95057397064189 19.738864408206798 14-15 25.311600101041286 23.06006343147436 29.406971848774877 22.221364618709476 16-17 21.05894075051222 26.757613180274497 29.709394032950687 22.474052036262595 18-19 21.31799481431044 25.419618163048902 33.545259146949014 19.717127875691645 20-21 22.815693254981014 24.6965587680307 32.92470335102407 19.563044625964213 22-23 21.51316572398571 26.67793655034904 30.688026118137866 21.120871607527377 24-25 20.67036048554979 27.603301702635136 31.156064643046694 20.57027316876838 26-27 21.573499627264482 26.777173766604694 31.255417014702847 20.39390959142798 28-29 21.148390356170534 27.46608100143142 31.52117656964832 19.86435207274973 30-31 22.16973320886411 27.42752876760312 31.06014209924493 19.342595924287835 32-33 19.601756995705745 28.17255606388055 31.502119060316037 20.723567880097672 34-35 19.13073619804092 29.193101125487665 31.128210165876112 20.5479525105953 36-37 20.711611327850907 29.159897836032446 29.936287855398692 20.192202980717955 38-39 20.44082641691499 28.74993088493387 29.92189787024555 20.88734482790559 40-41 19.633202265309038 29.270392987949585 29.90649719979982 21.189907546941562 42-43 20.430618063938347 28.758606267972088 29.7513227260075 21.059452942082064 44-45 20.717242807746068 28.324516996342417 29.885707965884333 21.07253223002718 46-47 19.802183624393187 28.03116359395124 30.69294836577172 21.47370441588385 48-49 19.708197874885652 28.485511223699948 30.20218140876786 21.604109492646543 50-51 18.59520522012552 28.413117310666998 30.100650122944877 22.8910273462626 52-53 18.88553954884399 27.497046535121957 29.719301909836915 23.898112006197138 54-55 19.301414388692546 26.971746571004047 29.771443435431387 23.955395604872017 56-57 20.022740382138636 25.807434173800363 29.87688720821131 24.292938235849686 58-59 19.41618953219662 25.811569630009775 30.778488087801563 23.993752749992048 60-61 19.986347008376182 25.478058797945575 30.39958224660681 24.136011947071434 62-63 18.9853708576404 25.53913767811383 31.197764373603192 24.27772709064258 64-65 18.914109904366356 25.634395003446052 31.032847882590893 24.4186472095967 66-67 19.380790064529428 25.459108368777873 30.838888172458397 24.321213394234306 68-69 19.757158636666826 25.39013897605571 30.704447746225032 24.148254641052432 70-71 19.243778993235207 25.416566356232266 30.617335050929544 24.722319599602983 72-73 19.91438825786694 25.175551376900597 30.417454388179028 24.49260597705344 74-75 20.174665455448597 24.758408738791964 30.442892438316015 24.624033367443428 76-77 19.737323737441997 25.09421318196153 29.978882281389378 25.1895807992071 78-79 20.177889228773125 24.89993062156768 29.501601560564218 25.420578589094973 80-81 19.776235870105722 25.3283734655162 28.549879295834984 26.34551136854309 82-83 19.739381776471575 25.173253682434165 27.639287046215838 27.448077494878415 84-85 20.55999433671128 24.7423668993184 27.385498201595592 27.312140562374722 86-87 20.87125001268745 24.218368495426315 27.123822745609296 27.786558746276942 88-89 21.158157076351955 24.336961928604627 26.755487442173926 27.749393552869495 90-91 21.86981132340289 24.437064993284107 26.428534335266928 27.264589348046076 92-93 21.65515633405387 25.03735415446459 25.849496256157224 27.45799325532432 94-95 21.741502708467824 25.063515675432935 26.3639170338769 26.831064582222346 96-97 22.10909652249572 25.074798619102417 26.078475399253414 26.737629459148447 98-99 22.495102304302677 25.263493221813693 25.904740520362623 26.336663953521004 100-101 22.558421510567232 25.190771562491225 26.1403351202672 26.11047180667434 102-103 22.38743720003368 25.93609138623032 26.487832945072835 25.188638468663164 104-105 22.312582446883155 26.004097785511803 26.909399646355496 24.773920121249542 106-107 21.40057256729069 26.6054618428808 27.405428162451933 24.588537427376576 108-109 21.231581015464933 27.206825900249797 27.295938701619466 24.265654382665804 110-111 20.933649555137666 27.434196862106713 27.75306632237784 23.879087260377784 112-113 20.217185461407286 27.837580404417412 27.642156810344876 24.303077323830426 114-115 20.18599643902427 27.59199351363574 28.130020892820028 24.091989154519965 116-117 19.962087850902503 27.834721926956263 28.49825483758123 23.70493538456001 118-119 19.540297748778123 28.510322692379493 28.55702165752368 23.392357901318704 120-121 19.530288993450306 28.973600194564835 28.6248455360444 22.871265275940456 122-123 19.05565132710125 29.734984577220164 28.229309691443195 22.98005440423539 124-125 18.45157719449759 30.690534643772434 28.6214764079703 22.236411753759672 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 66.0 1 40.5 2 37.0 3 89.0 4 136.0 5 257.5 6 528.0 7 942.0 8 1685.0 9 2199.0 10 2233.5 11 2321.0 12 2602.5 13 2947.5 14 3206.0 15 3601.0 16 4091.5 17 4735.5 18 5462.5 19 6089.0 20 6969.0 21 8174.0 22 9278.0 23 10593.5 24 12238.5 25 14167.0 26 16432.0 27 19029.5 28 22152.0 29 25705.0 30 29406.0 31 32932.5 32 36136.5 33 38153.0 34 39128.5 35 39071.0 36 38373.5 37 37380.5 38 35561.5 39 34155.0 40 32887.0 41 31909.5 42 31920.5 43 33710.5 44 37041.0 45 40622.0 46 46713.5 47 52811.0 48 66968.0 49 82122.0 50 98107.0 51 103337.5 52 85622.0 53 75204.5 54 73268.5 55 66639.5 56 56218.5 57 45140.0 58 36845.0 59 30559.0 60 23974.0 61 19165.5 62 15150.0 63 11066.0 64 7701.0 65 5767.5 66 4471.5 67 3401.0 68 2646.0 69 2102.0 70 1815.0 71 1565.5 72 1303.5 73 1324.0 74 1137.5 75 784.5 76 617.5 77 488.0 78 334.5 79 204.5 80 168.0 81 146.5 82 87.0 83 52.0 84 26.0 85 16.0 86 11.5 87 3.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12220382272867608 2 0.003087372645878358 3 0.0011788150102444639 4 0.0016278873950994976 5 0.00943052008195571 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.05206432961913E-4 20-21 5.332734570153527E-4 22-23 3.368042886412754E-4 24-25 3.0873726458783575E-4 26-27 0.00145948525077886 28-29 0.0 30-31 0.0010665469140307053 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.806702405343961E-5 40-41 0.003339975862359314 42-43 0.27564624322883047 44-45 0.2654859805214853 46-47 0.2780880743214797 48-49 0.28488029414241206 50-51 0.29456341744084874 52-53 0.28889387858205395 54-55 0.30014875522748324 56-57 0.29832439866400967 58-59 0.29798759437536837 60-61 0.2965281091245895 62-63 0.297706924134834 64-65 0.30907406887647704 66-67 0.31833618681411213 68-69 0.31662409834685223 70-71 0.32066574981054763 72-73 0.3232759830475175 74-75 0.3270930983187853 76-77 0.3194027337281428 78-79 0.3189536613432878 80-81 0.5480367116674618 82-83 0.5079570013191501 84-85 0.4843526340902074 86-87 0.4515422829717365 88-89 0.2989980072412922 90-91 0.012770495944315025 92-93 0.08574475848325802 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.2261640798226164 114-115 0.20020208257318478 116-117 0.19040669117853434 118-119 0.1705352381486991 120-121 0.1752785652137304 122-123 0.1634623480872323 124-125 6.455415532291111E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1781450.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.41815928687729 #Duplication Level Percentage of deduplicated Percentage of total 1 69.2383241806601 27.984856154903675 2 16.745993196527305 13.536844408684082 3 6.581444434839222 7.980296085151912 4 2.7404847412462554 4.430613951797914 5 1.3361057530738438 2.700146757592588 6 0.7375041734383598 1.7885136694061043 7 0.4572313279270589 1.2936314050174404 8 0.29911200276313116 0.9671645257837693 9 0.23905919879490234 0.8696099498297064 >10 1.3572102993517 10.457777261237453 >50 0.13335037129405838 3.7432496428253303 >100 0.10829101522864792 8.532028995527858 >500 0.015589258837698098 4.505193658054598 >1k 0.009186527529357807 6.646034050452415 >5k 8.351388663052553E-4 2.610650612530108 >10k+ 2.7837962210175177E-4 1.9533888712050516 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23058 1.2943388812484211 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 11664 0.6547475371186393 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9678 0.5432653175783773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9663 0.542423306856774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9136 0.5128406635044486 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 6871 0.3856970445423672 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 5577 0.31305958629206543 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 5480 0.30761458362569816 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 4908 0.27550590810856324 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4836 0.27146425664486795 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4452 0.24990878217182633 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3491 0.1959639619411154 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3308 0.18569143113755648 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 2941 0.16509023548233182 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 2871 0.16116085211485026 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 2856 0.16031884139324706 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 2810 0.15773667518033063 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 2798 0.15706306660304808 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2777 0.1558842515928036 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 2653 0.1489236296275506 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2616 0.14684666984759606 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2541 0.1426366162395801 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 2295 0.12882764040528782 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2279 0.12792949563557776 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 2192 0.12304583345027927 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 2158 0.12113727581464537 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2027 0.11378371551264418 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1869 0.10491453591175727 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1809 0.10154649302534452 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.613404810687923E-5 0.0 6 0.0 0.0 0.0 5.613404810687923E-5 0.0 7 0.0 0.0 0.0 5.613404810687923E-5 0.0 8 0.0 0.0 0.0 1.1226809621375845E-4 0.0 9 0.0 0.0 0.0 1.1226809621375845E-4 0.0 10-11 0.0 0.0 0.0 1.9646916837407727E-4 0.0 12-13 0.0 0.0 0.0 4.490723848550338E-4 0.0 14-15 0.0 0.0 0.0 7.858766734963092E-4 0.0 16-17 0.0 0.0 0.0 0.0024418310926492466 0.0 18-19 0.0 0.0 0.0 0.004631058968817536 0.0 20-21 0.0 0.0 0.0 0.009851525442757305 0.0 22-23 0.0 0.0 0.0 0.029442308232058154 0.0 24-25 0.0 0.0 0.0 0.07547222767969913 0.0 26-27 0.0 0.0 0.0 0.13772488703022817 0.0 28-29 0.0 0.0 0.0 0.2478318223918718 0.0 30-31 0.0 0.0 0.0 0.4394734626287575 0.0 32-33 0.0 0.0 0.0 0.7762777512700328 0.0 34-35 0.0 0.0 0.0 1.0930983187852592 0.0 36-37 0.0 0.0 0.0 1.5250217519436413 0.0 38-39 0.0 0.0 0.0 2.076286171377249 0.0 40-41 1.1226809621375845E-4 0.0 0.0 2.9032529680877937 0.0 42-43 1.1226809621375845E-4 0.0 0.0 3.605125038592158 0.0 44-45 1.684021443206377E-4 0.0 0.0 4.21721069914957 0.0 46-47 1.684021443206377E-4 0.0 0.0 4.8407477055207835 0.0 48-49 1.684021443206377E-4 0.0 0.0 5.421089561873755 0.0 50-51 1.684021443206377E-4 0.0 0.0 5.991888630048556 0.0 52-53 1.684021443206377E-4 0.0 0.0 6.632714923236689 0.0 54-55 1.684021443206377E-4 0.0 0.0 7.250947262061803 0.0 56-57 1.684021443206377E-4 0.0 0.0 7.854865418619664 0.0 58-59 1.684021443206377E-4 0.0 0.0 8.491846529512475 0.0 60-61 1.684021443206377E-4 0.0 0.0 9.082713519885488 0.0 62-63 1.684021443206377E-4 0.0 0.0 9.66993179713155 0.0 64-65 1.684021443206377E-4 0.0 0.0 10.272895674871593 0.0 66-67 1.684021443206377E-4 0.0 0.0 10.868590193381795 0.0 68-69 1.684021443206377E-4 0.0 0.0 11.527575851132504 0.0 70-71 1.684021443206377E-4 0.0 0.0 12.254118835779842 5.613404810687923E-5 72-73 1.684021443206377E-4 0.0 0.0 12.901849616885123 5.613404810687923E-5 74-75 1.684021443206377E-4 0.0 0.0 13.523113194308008 5.613404810687923E-5 76-77 1.684021443206377E-4 0.0 0.0 14.155828117544697 5.613404810687923E-5 78-79 1.684021443206377E-4 0.0 0.0 14.77462179685088 5.613404810687923E-5 80-81 2.806702405343961E-4 0.0 0.0 15.422099974739679 5.613404810687923E-5 82-83 2.806702405343961E-4 0.0 0.0 16.16646551966095 5.613404810687923E-5 84-85 2.806702405343961E-4 0.0 0.0 16.78913244828651 5.613404810687923E-5 86-87 2.806702405343961E-4 0.0 0.0 17.388840551236353 5.613404810687923E-5 88-89 2.806702405343961E-4 0.0 0.0 17.984198265457913 5.613404810687923E-5 90-91 2.806702405343961E-4 0.0 0.0 18.57969631479974 5.613404810687923E-5 92-93 3.368042886412754E-4 0.0 0.0 19.158859356142468 5.613404810687923E-5 94-95 3.368042886412754E-4 0.0 0.0 19.746470571725283 5.613404810687923E-5 96-97 3.368042886412754E-4 0.0 0.0 20.32844031547335 5.613404810687923E-5 98-99 3.368042886412754E-4 0.0 0.0 20.935305509556823 5.613404810687923E-5 100-101 3.368042886412754E-4 0.0 0.0 21.588986499761432 5.613404810687923E-5 102-103 3.368042886412754E-4 0.0 0.0 22.190968031659605 5.613404810687923E-5 104-105 5.052064329619131E-4 0.0 0.0 22.771001150747985 5.613404810687923E-5 106-107 5.052064329619131E-4 0.0 0.0 23.33523253529428 5.613404810687923E-5 108-109 5.052064329619131E-4 0.0 0.0 23.900979539139463 5.613404810687923E-5 110-111 5.052064329619131E-4 0.0 0.0 24.462235819136097 5.613404810687923E-5 112-113 5.052064329619131E-4 2.8067024053439613E-5 0.0 25.04268994358528 5.613404810687923E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAACG 400 0.0 62.345833 2 CGCATAG 605 0.0 58.944176 1 AGATTAC 955 0.0 47.252888 2 ATAACGC 515 0.0 46.118042 3 ACAACGC 505 0.0 45.855488 3 TAACGCA 570 0.0 42.70856 4 ATAGTAC 895 0.0 41.794918 4 GACCGTT 360 0.0 41.229385 7 TAGTACT 935 0.0 40.00577 5 GCATAGT 960 0.0 39.584656 2 CATGGGG 5845 0.0 37.48416 4 TATCACG 415 0.0 37.20004 2 GTGTACT 990 0.0 37.184544 4 AAACGCA 695 0.0 36.734776 5 GATTACT 1245 0.0 36.72311 3 GTATAAC 640 0.0 36.218433 1 GTACAAC 660 0.0 36.021442 1 CATGGGT 2965 0.0 35.44496 4 GGTTTCA 1115 0.0 35.181477 1 TCACGCA 440 0.0 35.085415 4 >>END_MODULE