FastQCFastQC Report
Fri 10 Feb 2017
SRR3558062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789371
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220470.7903932463627105No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA182060.6526919509810635No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT149130.5346366618137207No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT117680.4218872283392922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88900.3187098453378916No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG81770.29314852703351396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68820.24672228972051408No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62440.22384974963889712No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59550.21348899088719284No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC55000.19717706966911178No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44560.15974927680828402No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC38660.13859755478923386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37200.1333633998489265No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC36580.13114067651811107No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32470.1164061718573829No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA28260.10131316343361998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6850.059.8822671
TAACGCA6950.041.8628774
TAGTACT10200.038.420375
ATAGTAC11600.034.8071674
CATGGGG82050.034.4465834
TATCACG4150.034.3397032
CATGGGT37600.033.4784854
TACAACG5250.032.7998622
GTATAAC9700.032.482081
GCATAGT13350.031.135092
ACAACGC5950.028.940543
ATAACGC13300.028.5728723
ATCACGC6050.026.4992733
CATGGGC71850.026.3622024
AGATTAC11500.025.8169862
ATGGGTA16600.024.6807755
ACATGGG490050.023.8820613
GTACAAC9000.023.7793371
TCACGCA6300.023.5622774
ATGGGGG47650.023.4267865
Produced by FastQC (version 0.11.5)