FastQCFastQC Report
Fri 10 Feb 2017
SRR3558061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937769
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT519041.318106775689483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196330.4985818111727732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142860.3627942624364202No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132890.3374753572390864No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT110650.28099667603660855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80360.20407494700679496No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG65770.16702351001290325No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA61180.15536716348775156No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC60350.15325937097884615No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53580.13606689473150912No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52770.1340098924035412No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52090.13228302625166688No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA46920.11915376447932827No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41530.10546581071667739No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40270.10226602931761614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8800.056.7568321
TAACGCA10400.042.8293844
TATCACG6300.040.53662
TACAACG8000.038.604042
CATGGGG127550.034.642274
ATAACGC15800.033.8297923
AAACGCA12350.032.7006075
TAGTACT15900.032.4964565
AACGCAG646950.032.4772266
ATAGTAC15750.032.4288674
CATGGGT55800.032.249454
GTATAAC13600.031.9157831
ACAACGC10500.031.6747073
CATGGGC125650.031.5266084
AGATTAC18700.031.1246072
GATTACT18800.030.9586563
TCACGCA9450.030.1663884
CGCAGAG708850.029.6663028
ACATGGG601050.029.6629263
ACGCAGA709600.029.5847327
Produced by FastQC (version 0.11.5)