##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3558055.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2587197 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.702743548326627 33.0 32.0 34.0 27.0 34.0 2 31.888001570811962 33.0 32.0 34.0 27.0 34.0 3 32.022934859618346 33.0 32.0 34.0 27.0 34.0 4 31.85317005237715 33.0 32.0 34.0 27.0 34.0 5 32.03134357375956 33.0 32.0 34.0 28.0 34.0 6 35.38414005582103 38.0 36.0 38.0 29.0 38.0 7 35.76322135500312 38.0 36.0 38.0 31.0 38.0 8 35.96990062990951 38.0 37.0 38.0 31.0 38.0 9 36.07879144881507 38.0 37.0 38.0 32.0 38.0 10-11 36.04403066330086 38.0 37.0 38.0 31.5 38.0 12-13 36.04419145507667 38.0 37.0 38.0 31.5 38.0 14-15 36.060078532867806 38.0 37.0 38.0 31.5 38.0 16-17 36.00278718628694 38.0 37.0 38.0 31.0 38.0 18-19 35.992768042016124 38.0 37.0 38.0 31.0 38.0 20-21 35.960888753349664 38.0 37.0 38.0 31.0 38.0 22-23 35.89949798952303 38.0 37.0 38.0 31.0 38.0 24-25 35.79986564610272 38.0 37.0 38.0 31.0 38.0 26-27 35.68135592303176 38.0 37.0 38.0 29.0 38.0 28-29 35.58015759913141 38.0 37.0 38.0 29.0 38.0 30-31 35.458232403639926 38.0 37.0 38.0 29.0 38.0 32-33 35.30571850539406 38.0 36.5 38.0 27.5 38.0 34-35 35.058452448731195 38.0 36.0 38.0 27.0 38.0 36-37 34.837474108079135 38.0 36.0 38.0 25.0 38.0 38-39 34.60613378107658 38.0 35.5 38.0 24.0 38.0 40-41 34.275760794404135 38.0 35.0 38.0 16.0 38.0 42-43 33.74446901414929 38.0 34.0 38.0 15.5 38.0 44-45 33.28279852674535 38.0 33.5 38.0 15.0 38.0 46-47 32.57824123945722 38.0 30.0 38.0 15.0 38.0 48-49 31.962578999589134 38.0 27.0 38.0 15.0 38.0 50-51 31.459211842005075 38.0 25.0 38.0 15.0 38.0 52-53 31.0816547792843 38.0 24.5 38.0 15.0 38.0 54-55 30.8352154861033 38.0 24.0 38.0 15.0 38.0 56-57 30.58563843418186 38.0 24.0 38.0 15.0 38.0 58-59 30.406807057985922 37.5 16.0 38.0 15.0 38.0 60-61 30.216529510508863 37.0 16.0 38.0 15.0 38.0 62-63 30.069079007126245 37.0 15.5 38.0 15.0 38.0 64-65 29.918387737771805 37.0 15.0 38.0 15.0 38.0 66-67 29.692661981287085 37.0 15.0 38.0 15.0 38.0 68-69 29.540230025003893 37.0 15.0 38.0 14.5 38.0 70-71 29.32997583871657 36.0 15.0 38.0 14.0 38.0 72-73 29.12228137246603 36.0 15.0 38.0 14.0 38.0 74-75 28.948852561285438 36.0 15.0 38.0 14.0 38.0 76-77 28.810824997091444 36.0 14.0 38.0 14.0 38.0 78-79 28.60010544229914 36.0 14.0 38.0 14.0 38.0 80-81 28.521229732409246 36.0 14.0 38.0 14.0 38.0 82-83 28.40070914584394 35.0 14.0 38.0 13.5 38.0 84-85 28.296203961275467 35.0 14.0 38.0 13.0 38.0 86-87 28.197970235741614 35.0 14.0 38.0 13.0 38.0 88-89 28.099300130604668 34.5 14.0 38.0 13.0 38.0 90-91 28.067568878597186 35.0 14.0 38.0 13.0 38.0 92-93 28.00208874701076 34.5 14.0 38.0 13.0 38.0 94-95 27.929051981739313 34.0 14.0 38.0 13.0 38.0 96-97 27.84952324851954 34.0 14.0 38.0 13.0 38.0 98-99 27.758203376086165 34.0 14.0 38.0 13.0 38.0 100-101 27.694116837643207 34.0 14.0 38.0 13.0 38.0 102-103 27.61851938603825 34.0 14.0 38.0 13.0 38.0 104-105 27.442773202040662 34.0 14.0 38.0 13.0 38.0 106-107 27.288867063466757 33.5 14.0 38.0 13.0 38.0 108-109 27.12909936893093 33.0 14.0 38.0 13.0 38.0 110-111 26.987745231615527 32.0 14.0 38.0 13.0 38.0 112-113 26.679665870051643 31.0 14.0 38.0 13.0 38.0 114-115 26.569977469825453 30.0 14.0 38.0 13.0 38.0 116-117 26.46608163197468 28.5 14.0 38.0 13.0 38.0 118-119 26.30495629053373 27.5 14.0 38.0 13.0 38.0 120-121 26.2008523123674 27.0 14.0 38.0 13.0 38.0 122-123 26.05432114369335 25.0 14.0 38.0 13.0 38.0 124-125 25.162574979794734 24.0 14.0 36.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.44700362530016235 1101 2 -0.641324468416876 1101 3 -0.4233916702952918 1101 4 -0.4464208334131463 1101 5 -0.2063095803524213 1101 6 0.026813122253294353 1101 7 0.0980187558740866 1101 8 -0.11739451243725085 1101 9 -0.14357345262560983 1101 10-11 -0.08138001410669204 1101 12-13 -0.13303153092663678 1101 14-15 -0.1209038282754733 1101 16-17 -0.10666816228962261 1101 18-19 -0.11533212670227755 1101 20-21 -0.10509267380200527 1101 22-23 -0.12522777894882609 1101 24-25 -0.24442093143878907 1101 26-27 -0.10998323951681499 1101 28-29 -0.11912991864053168 1101 30-31 -0.17419072705173733 1101 32-33 -0.1640547793368654 1101 34-35 -0.2964117483913924 1101 36-37 -0.335569081233011 1101 38-39 -0.23753027718606745 1101 40-41 -0.4355712628120969 1101 42-43 -0.3733797521868851 1101 44-45 -0.3439239711550357 1101 46-47 -0.3139042708738202 1101 48-49 -0.27078749638532074 1101 50-51 -0.17432758223472788 1101 52-53 -0.1684695427955596 1101 54-55 -0.1040785496126837 1101 56-57 -0.18786019083182381 1101 58-59 -0.12984206454342484 1101 60-61 -0.3447619714529928 1101 62-63 -0.1275504970484107 1101 64-65 -0.18066538336133675 1101 66-67 -0.15463915210923318 1101 68-69 -0.29275981317481126 1101 70-71 -0.45135476946248687 1101 72-73 -0.4384722208957683 1101 74-75 -0.5168788589712392 1101 76-77 -0.5773988111484876 1101 78-79 -0.5009697742605717 1101 80-81 -0.5202154809191626 1101 82-83 -0.6681160806007256 1101 84-85 -0.47344573277020885 1101 86-87 -0.37418397665145164 1101 88-89 -0.3915307765014653 1101 90-91 -0.4532412845973681 1101 92-93 -0.3926699791671133 1101 94-95 -0.31386737021424693 1101 96-97 -0.45282014715396457 1101 98-99 -0.5075804859286883 1101 100-101 -0.5013110718391012 1101 102-103 -0.47486838639706264 1101 104-105 -0.6064366736714426 1101 106-107 -0.5015749308627271 1101 108-109 -0.36626532803366985 1101 110-111 -0.3418463815062971 1101 112-113 -0.5621881533921957 1101 114-115 -0.4878745140761396 1101 116-117 -0.20125536190949944 1101 118-119 -0.0519287721775612 1101 120-121 -0.2021887436162011 1101 122-123 -0.5088651691617763 1101 124-125 -0.5732656224069821 1102 1 -0.8763004336292255 1102 2 -0.8261256290244887 1102 3 -0.4746589514012811 1102 4 -0.53101860322597 1102 5 -0.3615024460291565 1102 6 -0.057794603752284956 1102 7 -0.15261701814878847 1102 8 -0.17131156312849782 1102 9 -0.2827908958753085 1102 10-11 -0.17951393912233726 1102 12-13 -0.12462921540066674 1102 14-15 -0.16376025997599442 1102 16-17 -0.17851916075352392 1102 18-19 -0.21351512152505592 1102 20-21 -0.20360564529884329 1102 22-23 -0.10115654950125474 1102 24-25 -0.24519538100356186 1102 26-27 -0.16790196853768435 1102 28-29 -0.048381924102208984 1102 30-31 -0.13133287303796948 1102 32-33 -0.10793100963783075 1102 34-35 -0.21495089045204452 1102 36-37 -0.17679198616559688 1102 38-39 -0.027042850435194055 1102 40-41 -0.045817607467050436 1102 42-43 -0.2778152360578545 1102 44-45 -0.18576842697798668 1102 46-47 -0.21648363595318898 1102 48-49 -0.3794970315733508 1102 50-51 -0.31280897837741506 1102 52-53 -0.40765498399712996 1102 54-55 -0.290967666071694 1102 56-57 -0.2737728892445226 1102 58-59 -0.2898437713193225 1102 60-61 -0.3955293094515149 1102 62-63 -0.19355578805370044 1102 64-65 -0.22394068631405872 1102 66-67 -0.1862770880482465 1102 68-69 -0.33483610605755487 1102 70-71 -0.24080973902498215 1102 72-73 -0.19217805806934862 1102 74-75 -0.40015387746131026 1102 76-77 -0.3375057691049079 1102 78-79 -0.3342191911121404 1102 80-81 -0.48189338386050906 1102 82-83 -0.3979619018444005 1102 84-85 -0.5389048554873952 1102 86-87 -0.3571894667805964 1102 88-89 -0.23555492738045558 1102 90-91 -0.36413051484143466 1102 92-93 -0.16621779733858588 1102 94-95 -0.058258477911806494 1102 96-97 -0.2614791902216105 1102 98-99 -0.3293717472360811 1102 100-101 -0.2458102753436826 1102 102-103 -0.21056773782222038 1102 104-105 -0.32117468357074586 1102 106-107 -0.14340303008330224 1102 108-109 -0.25948231614313144 1102 110-111 -0.282442757452138 1102 112-113 -0.4754341566919642 1102 114-115 -0.3785711631061197 1102 116-117 -0.2077852020414923 1102 118-119 -0.36436619039454854 1102 120-121 -0.14416120763167584 1102 122-123 -0.6436400169742562 1102 124-125 -0.5918680574072646 1103 1 -0.8928111393960698 1103 2 -0.8943576472999979 1103 3 -0.5393851759429573 1103 4 -0.5040572799417369 1103 5 -0.36718482924056417 1103 6 -0.2054585984709476 1103 7 -0.14081105115619152 1103 8 -0.14083931044242348 1103 9 -0.03200969073945714 1103 10-11 -0.02088949511676219 1103 12-13 0.03731146950135411 1103 14-15 -0.04718199683090063 1103 16-17 -0.1989005261222232 1103 18-19 -0.06629116017889913 1103 20-21 -0.1768317738969074 1103 22-23 -0.14968487714566692 1103 24-25 -0.2914731188631876 1103 26-27 -0.27135603208831327 1103 28-29 -0.12380971357531223 1103 30-31 -0.14266915984728 1103 32-33 -0.15494291458315246 1103 34-35 -0.2596951645323742 1103 36-37 -0.22979348064498595 1103 38-39 -0.10205301672345968 1103 40-41 -3.3986661939167107E-4 1103 42-43 -0.08361975479207473 1103 44-45 0.004692489888149964 1103 46-47 -0.038549547116396354 1103 48-49 -0.03448355511362067 1103 50-51 0.05173054128822585 1103 52-53 -0.029518082961921266 1103 54-55 0.1404989453165122 1103 56-57 0.1359574741786851 1103 58-59 0.1451267662870741 1103 60-61 0.06310545257796818 1103 62-63 0.13350339305002734 1103 64-65 0.2210065867162605 1103 66-67 0.19123256117162413 1103 68-69 0.2282765849717876 1103 70-71 0.3542566114712855 1103 72-73 0.18211129387004732 1103 74-75 0.06196006620414707 1103 76-77 -0.12791705674293397 1103 78-79 -0.01884645600009094 1103 80-81 -0.01258857122601853 1103 82-83 -0.06542529737066971 1103 84-85 -0.04264175428158623 1103 86-87 -0.01242180206688559 1103 88-89 0.07766036028931822 1103 90-91 -0.08745293721464975 1103 92-93 0.10132786225159762 1103 94-95 -0.012491972458423817 1103 96-97 0.16455084970120382 1103 98-99 0.03354653541521557 1103 100-101 -0.021020919435819962 1103 102-103 -0.04723598720839206 1103 104-105 -0.0526144961095234 1103 106-107 0.04846996204559062 1103 108-109 -0.016091804149709787 1103 110-111 -0.002926556053644447 1103 112-113 -0.22623800545494532 1103 114-115 -0.07078310932353915 1103 116-117 -0.017309345050236402 1103 118-119 -0.07408656260909297 1103 120-121 -0.07123822350976283 1103 122-123 -0.3277455446804858 1103 124-125 -0.5613157349881597 1104 1 -0.23294252916359426 1104 2 -0.07066334233122262 1104 3 -0.17616196080142998 1104 4 -0.4006399109812797 1104 5 0.03327562479882573 1104 6 0.059648552022686374 1104 7 0.09353388016279496 1104 8 0.01691965348068436 1104 9 0.04452927274126495 1104 10-11 0.025972486642032777 1104 12-13 0.10789015221202192 1104 14-15 0.15096462036184022 1104 16-17 0.10164458104670615 1104 18-19 0.11649439321386268 1104 20-21 0.02959398387993417 1104 22-23 0.007038618176061107 1104 24-25 -0.08344646303507375 1104 26-27 -0.006999561768985529 1104 28-29 -0.05174073026077508 1104 30-31 0.01548058172334521 1104 32-33 0.12906441802761037 1104 34-35 -0.00446805267411321 1104 36-37 -0.006561519088656098 1104 38-39 0.053576715925643725 1104 40-41 -0.018494668023244287 1104 42-43 0.19295934634561007 1104 44-45 0.034610021058199436 1104 46-47 -0.10989296509364266 1104 48-49 0.034081101997433194 1104 50-51 -0.11807571041681797 1104 52-53 -0.06269685636249633 1104 54-55 -0.10876558165820427 1104 56-57 -0.12361565654012097 1104 58-59 -0.16612163926639667 1104 60-61 0.033030780868060106 1104 62-63 -0.10273206338563412 1104 64-65 -0.11746906709898397 1104 66-67 0.05546866460235478 1104 68-69 -0.19576969637337527 1104 70-71 -0.103205997375035 1104 72-73 -0.16731880669989962 1104 74-75 -0.09782375265197985 1104 76-77 -0.33794276143300195 1104 78-79 -0.08301716869004139 1104 80-81 -0.048755088586133866 1104 82-83 -0.21205700602750355 1104 84-85 -0.1139144359747597 1104 86-87 -0.061472461227673136 1104 88-89 -0.0696941487686118 1104 90-91 -0.10216124566355589 1104 92-93 -0.012752713518715098 1104 94-95 0.08587431891423591 1104 96-97 0.01866083517607464 1104 98-99 -0.11827979709969938 1104 100-101 0.007533267783536246 1104 102-103 -0.11123780837969122 1104 104-105 -0.05249836878974179 1104 106-107 -0.022664703249670737 1104 108-109 0.061596681550035726 1104 110-111 -0.03462677982914997 1104 112-113 -0.28018006713882926 1104 114-115 0.061039831536319866 1104 116-117 -0.0882218938052759 1104 118-119 -0.1476778737049642 1104 120-121 0.004158590915096738 1104 122-123 -0.2091590445735747 1104 124-125 -0.17865271435067598 1105 1 0.06580321959623703 1105 2 0.05775795827749164 1105 3 -0.011770745614665401 1105 4 -0.018155110744437764 1105 5 0.06864485990053026 1105 6 0.22861819943150152 1105 7 0.08994875787871592 1105 8 0.019734859020474005 1105 9 0.07530969573515023 1105 10-11 0.1424167529736522 1105 12-13 0.09058823653641213 1105 14-15 0.03651261514843185 1105 16-17 0.05673961567039498 1105 18-19 -0.0410034495750935 1105 20-21 -0.08667856303714672 1105 22-23 0.04612971927434728 1105 24-25 -0.0734445404997146 1105 26-27 -0.08179701860670718 1105 28-29 -0.09477663924993607 1105 30-31 -0.013468468381717003 1105 32-33 0.08556502457398096 1105 34-35 0.015937448845861013 1105 36-37 -0.08220358271825745 1105 38-39 -0.030877870720225076 1105 40-41 0.06604701940319302 1105 42-43 0.06738597048182271 1105 44-45 0.14797732820895249 1105 46-47 0.08616976371514085 1105 48-49 0.03557287210191262 1105 50-51 0.04375257558423584 1105 52-53 -0.06278847013687638 1105 54-55 -0.05124837812579841 1105 56-57 -0.06481766536392897 1105 58-59 0.02083950927247713 1105 60-61 0.05079618100814187 1105 62-63 -0.030546050417097348 1105 64-65 -0.13651525488284832 1105 66-67 -0.10364686081843644 1105 68-69 -0.16847601239847165 1105 70-71 -0.13409947086390517 1105 72-73 -0.022894204384915184 1105 74-75 -0.05010877167876515 1105 76-77 -0.09998004417747808 1105 78-79 -0.1657270319282773 1105 80-81 -0.20346976809136308 1105 82-83 -0.34295616588857314 1105 84-85 -0.32911564329086573 1105 86-87 -0.2141341798479246 1105 88-89 -0.22879523260920465 1105 90-91 -0.31240334972958195 1105 92-93 -0.11026970945340864 1105 94-95 -0.06426305661739562 1105 96-97 -0.10451952571901657 1105 98-99 -0.10331290442229957 1105 100-101 -0.16867138565464046 1105 102-103 -0.17813752604680033 1105 104-105 -0.09974941399910975 1105 106-107 -0.1526093649195488 1105 108-109 -0.07893230450661548 1105 110-111 -0.047234504991436665 1105 112-113 -0.25883541160662205 1105 114-115 -0.164654988990943 1105 116-117 -0.15280667129663073 1105 118-119 -0.3468080844076198 1105 120-121 -0.11517782750080485 1105 122-123 -0.2530278265707011 1105 124-125 -0.27768810705334346 1106 1 0.2559632437740902 1106 2 0.13706585863116771 1106 3 0.05467640257143813 1106 4 0.09781854018864422 1106 5 -0.1420265293244043 1106 6 0.1927713623726035 1106 7 -0.040595817472031115 1106 8 -0.015253253249014165 1106 9 -0.0036219239156878302 1106 10-11 -0.13061299589001152 1106 12-13 -0.025935280793476068 1106 14-15 -0.0028855583276694574 1106 16-17 -0.08000096297141823 1106 18-19 -0.1939395092526084 1106 20-21 -0.2271697484797457 1106 22-23 -0.031070966335633443 1106 24-25 -0.10348000249449996 1106 26-27 -0.03473688817985732 1106 28-29 -0.05785768043876516 1106 30-31 -0.17366473365661506 1106 32-33 -0.045181603668389414 1106 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16-17 0.08312357095848455 2315 18-19 -0.10948760768661714 2315 20-21 0.09162219782897552 2315 22-23 -3.1033993742823895E-4 2315 24-25 0.10326869250922499 2315 26-27 0.1250319410520646 2315 28-29 0.18077020919583475 2315 30-31 0.17551498297276424 2315 32-33 0.30642167746508875 2315 34-35 0.18014758899009564 2315 36-37 0.1823626328396415 2315 38-39 0.10732031405714793 2315 40-41 0.16376292276369497 2315 42-43 0.2980128652627769 2315 44-45 0.18597375974940178 2315 46-47 0.26263642370378903 2315 48-49 0.30227498364024896 2315 50-51 0.21589693038911406 2315 52-53 0.2922700438668464 2315 54-55 0.21136301851344896 2315 56-57 0.14960518681811763 2315 58-59 0.10049244062329521 2315 60-61 0.13886298859813806 2315 62-63 0.19340667776782539 2315 64-65 0.12356919637659658 2315 66-67 0.22678196219778357 2315 68-69 0.1773238131168675 2315 70-71 0.1872677936567193 2315 72-73 0.32513569430610545 2315 74-75 0.17263056896100792 2315 76-77 0.01593239157868709 2315 78-79 0.30391730361308333 2315 80-81 0.11342279542045475 2315 82-83 0.2269515661447734 2315 84-85 0.16697985580974262 2315 86-87 0.19549616609916143 2315 88-89 0.25041686385221595 2315 90-91 0.19659891994082201 2315 92-93 0.2744867269157716 2315 94-95 -0.060515660402803206 2315 96-97 0.07244884187341327 2315 98-99 0.2199815679756476 2315 100-101 0.2078145806590328 2315 102-103 0.0755191175978247 2315 104-105 0.03887599278216314 2315 106-107 0.06193977031976061 2315 108-109 -0.06979947099734218 2315 110-111 0.21578784668162143 2315 112-113 0.20977040631803945 2315 114-115 0.33043538258946015 2315 116-117 0.3239630252216372 2315 118-119 0.1311608730765137 2315 120-121 -0.024421618869208572 2315 122-123 -0.12051558995649359 2315 124-125 0.08512097608029023 2316 1 -0.1686718836377068 2316 2 -0.04046715078802876 2316 3 -0.007529421607635811 2316 4 0.25073366477359826 2316 5 0.2031741253070649 2316 6 -0.30314056324502303 2316 7 -0.4377890014928525 2316 8 -0.3755379375881489 2316 9 -0.1429956136777335 2316 10-11 -0.339123336891312 2316 12-13 -0.17125230816137105 2316 14-15 -0.011951693876035563 2316 16-17 -0.03810912378366993 2316 18-19 -0.07791514326189741 2316 20-21 -0.12102225640972364 2316 22-23 -0.22874443531644317 2316 24-25 -0.05406278988618851 2316 26-27 -0.10701297879487726 2316 28-29 0.024951484401746882 2316 30-31 0.08900804767377934 2316 32-33 0.06612759181581396 2316 34-35 -0.05221674374121221 2316 36-37 -0.07740302938823618 2316 38-39 -0.0025838166251759276 2316 40-41 -0.06498721322687118 2316 42-43 -0.15861318164465388 2316 44-45 -0.14850548655055462 2316 46-47 -0.42428719255737235 2316 48-49 -0.16095214069369135 2316 50-51 -0.06897449657786936 2316 52-53 -0.20876854285173962 2316 54-55 -0.07032982138892407 2316 56-57 -0.06463669839382291 2316 58-59 -0.11410917812630927 2316 60-61 -0.09584485381290975 2316 62-63 -0.048072908398584246 2316 64-65 -0.020606023400286944 2316 66-67 -0.06534454319213268 2316 68-69 -0.08100064234171356 2316 70-71 0.021944171296144788 2316 72-73 0.06920782516906243 2316 74-75 0.0177382661590606 2316 76-77 0.21219603058949232 2316 78-79 0.02376713190574975 2316 80-81 -0.14411621371195338 2316 82-83 -0.13646991840009903 2316 84-85 -0.047572498432561616 2316 86-87 -0.08814753363014916 2316 88-89 -0.10073570568771828 2316 90-91 -0.08348615223901135 2316 92-93 -0.12580638224059726 2316 94-95 -0.1655814593103635 2316 96-97 -0.29556993024979406 2316 98-99 -0.24582006672627088 2316 100-101 -0.2631163543138726 2316 102-103 -0.10433731448474859 2316 104-105 -0.3456875896624325 2316 106-107 -0.262618395961443 2316 108-109 -0.3175931248490791 2316 110-111 -0.38107115976469785 2316 112-113 -0.4260979524671562 2316 114-115 -0.33449471019495647 2316 116-117 -0.45711598771703876 2316 118-119 -0.7065930700634482 2316 120-121 -0.633580550568368 2316 122-123 -0.6466878726303698 2316 124-125 -0.430527542773504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 13.0 10 13.0 11 37.0 12 88.0 13 233.0 14 604.0 15 3968.0 16 8166.0 17 10481.0 18 15994.0 19 26604.0 20 44953.0 21 78007.0 22 128877.0 23 195020.0 24 202446.0 25 131401.0 26 85190.0 27 65839.0 28 50516.0 29 48822.0 30 54133.0 31 62669.0 32 77084.0 33 101002.0 34 137552.0 35 179368.0 36 280341.0 37 597776.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.20895610183489 15.342337886693091 11.239631489776032 21.20907452169599 2 14.515428914666986 24.49663197304496 36.95309422860838 24.03484488367967 3 20.15191900951156 35.463989968954465 25.84915243231209 18.534938589221884 4 13.0495332702008 18.601047484683917 33.774964729528634 34.57445451558665 5 12.814989941660082 39.55339733767108 29.20811254207726 18.423500178591585 6 27.595772567763493 34.16322761660592 20.657414182221146 17.583585633409438 7 25.201637138571204 35.56114976942227 21.719181028734962 17.518032063271562 8 29.796803258507182 27.052018072067956 22.289489358560637 20.86168931086423 9 33.04216880276222 14.437478089221656 20.938220011850664 31.582133096165464 10-11 23.300796189853344 27.64681236102237 25.71603553962068 23.336355909503606 12-13 26.94282654162014 26.086629661367112 28.873719318629387 18.096824478383365 14-15 25.75176919268227 23.394005172393133 31.034745324766533 19.81948031015806 16-17 19.02628597667669 27.747345872772733 30.85174805010983 22.374620100440747 18-19 19.662718751014616 25.229283685607694 36.986469479604885 18.121528083772805 20-21 22.126495815032335 24.980205254647295 35.688674506745095 17.204624423575275 22-23 20.012148310658162 27.635512801335505 33.283762196142455 19.06857669186388 24-25 18.782477346060666 28.942562349547075 34.109968042523384 18.16499226186887 26-27 19.714362687763657 28.05369358393483 34.29669266941923 17.935251058882283 28-29 19.136443803854135 28.676150289289914 35.09323024106784 17.094175665788107 30-31 20.13915999205308 29.107834595847347 33.80179315900101 16.951212253098564 32-33 17.201299321234526 29.96886205418451 34.36586004080864 18.463978583772324 34-35 16.605113564989445 31.189565386787322 33.67416551580726 18.53115553241597 36-37 17.967244086940422 31.839883085826088 32.050632402557675 18.14224042467582 38-39 17.968249035539234 31.044678855147094 32.13419774373579 18.852874365577883 40-41 16.991246048932837 32.20769833608253 31.93554216227133 18.8655134527133 42-43 18.02688707421079 31.768603088501674 31.52695897825707 18.67755085903047 44-45 18.064183057251128 30.93933968944482 31.532730072489745 19.463747180814305 46-47 16.998273693684997 30.434797776791907 32.59757448826656 19.969354041256533 48-49 16.984643307933965 30.36094467652249 32.20518981296267 20.449222202580874 50-51 16.210197552574975 29.457978788569584 32.15232514681914 22.1794985120363 52-53 16.652274546221772 28.362595373238563 31.692961119177802 23.292168961361856 54-55 17.332068879151315 27.44523390980761 31.854223062434624 23.368474148606452 56-57 17.8819408770011 25.813923102959098 32.03823024138253 24.26590577865727 58-59 17.36889772159378 25.724352664266277 32.89347936244792 24.013270251692028 60-61 17.95106551091254 25.49142683010988 32.60250933771071 23.954998321266874 62-63 16.85062213637763 25.79053229937294 33.34931288570943 24.009532678539998 64-65 16.657761433853636 25.40953731046976 33.3229288160409 24.609772439635705 66-67 17.502082399420495 24.809656283882617 33.181192792183516 24.50706852451337 68-69 18.00234024257477 24.062327620388224 33.54798806030342 24.387344076733587 70-71 17.441222868364353 23.790390130932973 33.720902275699146 25.047484725003528 72-73 18.25155866381298 23.43296940919884 33.30322715249175 25.012244774496434 74-75 18.316946875610807 22.92061591156587 32.91985191688979 25.842585295933528 76-77 17.899022504462465 23.279591179079027 32.184855896287054 26.636530420171457 78-79 18.369700162462653 23.0641709062911 31.254390456758742 27.311738474487505 80-81 17.912705965065832 23.061227781384623 30.41115381244633 28.61491244110322 82-83 18.261113647179947 23.021163764216823 28.783981973406892 29.933740615196342 84-85 18.852387065200293 22.513628304147627 27.886549587218234 30.747435043433846 86-87 19.304086353182466 21.725673561579526 27.31231194500844 31.65792814022957 88-89 19.672563338693944 21.703243660702793 26.79241874177105 31.831774258832212 90-91 20.547678845683276 21.77997364393646 26.159301490868735 31.513046019511528 92-93 20.36364079494343 22.19697100115729 25.504992720259306 31.934395483639978 94-95 20.569249268610005 22.199063310602167 25.929007338830402 31.302680081957423 96-97 21.35034556703645 22.04708416096648 25.567593036015424 31.034977235981643 98-99 21.842937651986485 22.252338487756337 25.473567807303315 30.431156052953867 100-101 22.08177421356008 22.32951336910177 25.65444378607427 29.934268631263873 102-103 22.097447546514626 22.756345960512476 26.214567348369684 28.931639144603217 104-105 21.91541656858755 22.737387218677203 26.972221288135383 28.374974924599865 106-107 20.998478275910184 23.43895729625537 27.81879385296133 27.743770574873118 108-109 20.702404957952563 23.886024141184457 28.272914664016696 27.138656236846288 110-111 20.10036344352595 24.314692696381453 29.004092073390623 26.58085178670198 112-113 19.494988686505298 24.738557641291806 29.219964516634544 26.54648915556835 114-115 19.41211796217574 24.76529046968631 29.672892988256727 26.14969857988122 116-117 19.199076417469527 25.131761936610975 30.08891147154721 25.58025017437229 118-119 18.685056461867525 25.989515330895507 30.402481503336908 24.92294670390006 120-121 18.418407294023073 26.820853297853475 30.392879297486385 24.367860110637064 122-123 17.74853921169312 27.908277361614214 30.238472819968003 24.10471060672466 124-125 16.958663228184424 29.370807223904873 30.769062781676475 22.901466766234226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 65.0 1 43.5 2 34.5 3 82.0 4 138.0 5 306.5 6 681.0 7 1247.0 8 2217.5 9 3039.0 10 3462.5 11 4031.0 12 4695.0 13 5453.5 14 6260.5 15 7205.5 16 8282.5 17 9400.0 18 10751.5 19 12273.0 20 13895.0 21 15665.5 22 18074.5 23 20948.5 24 24254.5 25 27993.0 26 32494.5 27 37792.0 28 43176.0 29 49043.5 30 55053.5 31 60881.5 32 66208.0 33 69139.5 34 69871.5 35 68539.0 36 65318.5 37 61706.5 38 57121.0 39 51980.5 40 46952.5 41 42820.0 42 40434.5 43 40643.0 44 42587.0 45 45059.0 46 51057.0 47 57957.5 48 75813.0 49 95957.0 50 121441.0 51 131805.5 52 122227.0 53 125172.5 54 112885.5 55 87322.5 56 70036.0 57 58187.5 58 49148.0 59 40658.5 60 31914.5 61 25525.0 62 20617.5 63 14743.5 64 9723.0 65 7535.0 66 5725.0 67 4119.5 68 3206.0 69 2710.0 70 2514.5 71 2290.5 72 1966.0 73 2093.0 74 1696.5 75 1227.0 76 981.0 77 581.0 78 375.5 79 227.5 80 151.5 81 119.5 82 84.5 83 51.0 84 29.0 85 18.5 86 7.0 87 3.5 88 3.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12256507718585016 2 0.0028602383196950215 3 0.0011209042063669678 4 0.0018166378516981893 5 0.011170390194484611 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.478668226656107E-4 20-21 7.92363318293891E-4 22-23 2.3191121511040712E-4 24-25 3.478668226656107E-4 26-27 0.001352815421477375 28-29 0.0 30-31 0.0011982079447371034 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0030728236002128947 42-43 0.2848642758939501 44-45 0.2747374861674623 46-47 0.28646832846513043 48-49 0.293947465152441 50-51 0.3027600913266365 52-53 0.2960926438922123 54-55 0.3074756193672148 56-57 0.30650932263758807 58-59 0.30511785534692565 60-61 0.3042288623556691 62-63 0.30376503992544823 64-65 0.3165201567565207 66-67 0.32626042779115777 68-69 0.32484963456590277 70-71 0.32749728760507996 72-73 0.3303188740555899 74-75 0.3341454091049116 76-77 0.3271494207824143 78-79 0.3266276205484159 80-81 0.5472138379875982 82-83 0.5110550143649673 84-85 0.48670433677837444 86-87 0.45686509376750206 88-89 0.3049245960010003 90-91 0.012213990662481442 92-93 0.08826154328410245 94-95 0.0 96-97 0.0 98-99 5.797780377760178E-5 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.2314280667455938 114-115 0.20622704803692954 116-117 0.19627419172177457 118-119 0.17660039030657504 120-121 0.18162513330063385 122-123 0.16794237160911982 124-125 3.478668226656107E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2587197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.56289544405242 #Duplication Level Percentage of deduplicated Percentage of total 1 73.74477106860242 26.225775830582897 2 13.897303837188076 9.884567266322964 3 5.242121744383587 5.592750825015217 4 2.296465461331291 3.2667584436880928 5 1.1350660627909606 2.0183117856563584 6 0.6845398054104698 1.4606530516262717 7 0.4557171300444208 1.1344634453483367 8 0.32384261684555027 0.9213424898565307 9 0.24845418401047956 0.795217515174184 >10 1.6016253575217145 10.93443373621525 >50 0.1823405918778785 4.544128217310775 >100 0.15455189478627157 10.956839595259826 >500 0.01812908126430402 4.435846990762869 >1k 0.013323782615934282 8.809124816270607 >5k 0.001092113329174941 2.8678116655950294 >10k+ 6.552679975049647E-4 6.151974325314826 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59693 2.3072460272642554 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25511 0.986047834780266 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23832 0.9211513464185371 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 19127 0.7392943019027929 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 17474 0.6754027621398757 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12761 0.4932365026706509 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 9439 0.3648349932378555 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8669 0.33507305396535325 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 8542 0.3301642665788496 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 8107 0.3133507034833451 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 7917 0.3060068483381822 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 7887 0.3048472922626302 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 6154 0.23786360296490758 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5838 0.22564961230242617 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 5741 0.22190038099147458 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 5545 0.21432461463120125 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4981 0.19252496041082298 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4962 0.19179057489630671 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 4648 0.1796538879721954 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4583 0.17714151647516596 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4565 0.17644578282983475 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 4463 0.17250329217295785 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3863 0.14931217066191713 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 3441 0.1330010818658185 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3290 0.12716464961887325 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3121 0.12063248372659678 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 3116 0.12043922438067142 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 3033 0.1172311192383108 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 3007 0.11622617063949904 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 2987 0.11545313325579769 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 2955 0.11421627344187552 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2921 0.11290210988958321 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 2814 0.10876635988678095 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 2739 0.10586746969790085 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA 2738 0.1058288178287158 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 2721 0.10517173605256963 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 2606 0.10072677109628683 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA 2589 0.10006968932014068 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.865186918506785E-5 0.0 3 0.0 0.0 0.0 3.865186918506785E-5 0.0 4 0.0 0.0 0.0 7.73037383701357E-5 0.0 5 0.0 0.0 0.0 1.1595560755520357E-4 0.0 6 0.0 0.0 0.0 1.1595560755520357E-4 0.0 7 0.0 0.0 0.0 1.1595560755520357E-4 0.0 8 0.0 0.0 0.0 1.546074767402714E-4 0.0 9 0.0 0.0 0.0 1.546074767402714E-4 0.0 10-11 0.0 0.0 0.0 5.21800233998416E-4 0.0 12-13 0.0 0.0 0.0 8.503411220714928E-4 0.0 14-15 3.865186918506785E-5 0.0 0.0 0.0012948376176997731 1.9325934592533926E-5 16-17 3.865186918506785E-5 0.0 0.0 0.002666978973769682 7.73037383701357E-5 18-19 3.865186918506785E-5 0.0 0.0 0.004174401871987328 7.73037383701357E-5 20-21 3.865186918506785E-5 0.0 0.0 0.008870603977973074 7.73037383701357E-5 22-23 3.865186918506785E-5 0.0 0.0 0.02740417525221311 7.73037383701357E-5 24-25 3.865186918506785E-5 0.0 0.0 0.07024977224386084 7.73037383701357E-5 26-27 7.73037383701357E-5 0.0 0.0 0.13025679915367866 7.73037383701357E-5 28-29 7.73037383701357E-5 0.0 0.0 0.2830283121076594 7.73037383701357E-5 30-31 7.73037383701357E-5 0.0 0.0 0.7196591523567784 7.73037383701357E-5 32-33 7.73037383701357E-5 0.0 0.0 1.4987069017164136 7.73037383701357E-5 34-35 7.73037383701357E-5 0.0 0.0 2.0335714674993826 7.73037383701357E-5 36-37 7.73037383701357E-5 0.0 0.0 2.6082474585429716 7.73037383701357E-5 38-39 7.73037383701357E-5 0.0 0.0 3.259473476507587 7.73037383701357E-5 40-41 7.73037383701357E-5 0.0 0.0 4.242950961987046 7.73037383701357E-5 42-43 7.73037383701357E-5 0.0 0.0 5.066197123759807 7.73037383701357E-5 44-45 7.73037383701357E-5 0.0 0.0 5.745793613706262 7.73037383701357E-5 46-47 1.9325934592533925E-4 0.0 0.0 6.38697014568276 7.73037383701357E-5 48-49 3.092149534805428E-4 0.0 0.0 6.944523358677364 7.73037383701357E-5 50-51 3.092149534805428E-4 0.0 0.0 7.49065494432778 7.73037383701357E-5 52-53 3.092149534805428E-4 1.9325934592533926E-5 0.0 8.162347127025889 9.662967296266964E-5 54-55 3.092149534805428E-4 3.865186918506785E-5 0.0 8.753160273454244 1.1595560755520357E-4 56-57 3.092149534805428E-4 3.865186918506785E-5 0.0 9.284236956057075 1.352815421477375E-4 58-59 3.092149534805428E-4 3.865186918506785E-5 0.0 9.852458084946758 1.546074767402714E-4 60-61 3.092149534805428E-4 3.865186918506785E-5 0.0 10.351473042060578 1.546074767402714E-4 62-63 3.092149534805428E-4 3.865186918506785E-5 0.0 10.864692561099908 1.546074767402714E-4 64-65 3.092149534805428E-4 7.73037383701357E-5 0.0 11.405567492541156 1.546074767402714E-4 66-67 3.092149534805428E-4 7.73037383701357E-5 0.0 11.910708770920808 1.546074767402714E-4 68-69 3.092149534805428E-4 7.73037383701357E-5 0.0 12.47145076312318 1.546074767402714E-4 70-71 3.092149534805428E-4 7.73037383701357E-5 0.0 13.105264114019922 1.546074767402714E-4 72-73 3.092149534805428E-4 7.73037383701357E-5 0.0 13.637867545455563 1.546074767402714E-4 74-75 3.092149534805428E-4 7.73037383701357E-5 0.0 14.143511298134623 1.546074767402714E-4 76-77 3.092149534805428E-4 7.73037383701357E-5 0.0 14.653039563666779 1.546074767402714E-4 78-79 3.092149534805428E-4 7.73037383701357E-5 0.0 15.140497611894261 1.546074767402714E-4 80-81 4.638224302208143E-4 7.73037383701357E-5 0.0 15.668520796831473 1.9325934592533928E-4 82-83 4.638224302208143E-4 7.73037383701357E-5 0.0 16.29931930193178 2.3191121511040714E-4 84-85 4.638224302208143E-4 7.73037383701357E-5 0.0 16.81451006629955 2.3191121511040714E-4 86-87 4.638224302208143E-4 7.73037383701357E-5 0.0 17.30011282480615 2.3191121511040714E-4 88-89 4.638224302208143E-4 7.73037383701357E-5 0.0 17.795069335655537 2.3191121511040714E-4 90-91 4.638224302208143E-4 7.73037383701357E-5 0.0 18.26648685817122 2.3191121511040714E-4 92-93 4.638224302208143E-4 7.73037383701357E-5 1.9325934592533926E-5 18.714017525530526 2.3191121511040714E-4 94-95 4.638224302208143E-4 7.73037383701357E-5 3.865186918506785E-5 19.177356807386527 2.70563084295475E-4 96-97 4.638224302208143E-4 7.73037383701357E-5 3.865186918506785E-5 19.634956286668544 2.70563084295475E-4 98-99 4.638224302208143E-4 7.73037383701357E-5 3.865186918506785E-5 20.11205563395443 2.70563084295475E-4 100-101 4.831483648133482E-4 7.73037383701357E-5 3.865186918506785E-5 20.67063312148244 2.70563084295475E-4 102-103 5.024742994058821E-4 7.73037383701357E-5 3.865186918506785E-5 21.129933283008597 2.70563084295475E-4 104-105 5.797780377760179E-4 7.73037383701357E-5 3.865186918506785E-5 21.58411207186774 2.70563084295475E-4 106-107 6.184299069610856E-4 7.73037383701357E-5 3.865186918506785E-5 22.026192825671956 2.70563084295475E-4 108-109 6.184299069610856E-4 7.73037383701357E-5 3.865186918506785E-5 22.468795379710166 2.70563084295475E-4 110-111 6.184299069610856E-4 7.73037383701357E-5 3.865186918506785E-5 22.91669323982673 3.092149534805428E-4 112-113 6.184299069610856E-4 7.73037383701357E-5 3.865186918506785E-5 23.396904062582017 3.478668226656107E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 1000 0.0 73.129944 1 TACAACG 650 0.0 59.38399 2 TAGTACT 1320 0.0 58.032116 5 ATAGTAC 1310 0.0 58.02293 4 GTACAAC 690 0.0 57.7319 1 AGATTAC 1585 0.0 56.574024 2 GCATAGT 1415 0.0 52.879032 2 TATCACG 635 0.0 50.49977 2 AGTGTAC 1375 0.0 50.097527 3 CATAGTA 1570 0.0 48.035805 3 GTATAAC 795 0.0 47.86334 1 GATTACT 1875 0.0 47.822983 3 TCACGCA 685 0.0 47.679657 4 GTGTACT 1480 0.0 46.54331 4 ATAACGC 910 0.0 44.37399 3 ACAACGC 985 0.0 42.201004 3 TAACGCA 1060 0.0 40.335518 4 GGTTTCA 1540 0.0 38.993374 1 ATTACTT 2350 0.0 38.40933 4 GTATCAC 950 0.0 38.17652 1 >>END_MODULE