FastQCFastQC Report
Fri 10 Feb 2017
SRR3558052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335598
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT685892.056272968145442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277440.8317549057170559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260180.7800100611644448No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA156120.4680420122568727No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149980.4496345183082614No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT140200.42031443837057103No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG87200.2614223896284864No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA77520.23240210600917735No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG64110.19219941971424614No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA63590.19064047885866342No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA63350.18992096769454833No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC63110.18920145653043322No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62580.1876125360430124No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT61500.18437473580449443No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55100.16518777142809177No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA53000.15889204874208462No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG52300.15679347451341558No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50540.15151705930990486No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC49600.1486989739171207No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA44070.13212023751063529No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC39070.1171304215915707No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC38950.11677066600951315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10000.065.397941
TACAACG7100.047.678812
AGATTAC17150.046.749672
TAGTACT14750.045.4969525
ATAGTAC14450.044.7975734
GCATAGT15250.042.838252
TATCACG6450.041.434462
GATTACT19950.039.8900223
GTATAAC9000.039.6351131
TCACGCA7550.036.9697724
TAACGCA9950.036.4084854
ACAACGC9800.036.360313
GTACAAC11300.036.302961
ATAACGC9500.036.2582443
CATAGTA18400.035.826763
AAACGCA10900.034.324955
CATGGGG130250.033.6491784
GGTTTCA17500.033.633231
CATGGGT42900.033.50074
GAACAGT37050.032.2536771
Produced by FastQC (version 0.11.5)