FastQCFastQC Report
Fri 10 Feb 2017
SRR3558050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059950
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363891.1892024379483324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161830.5288648507328552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134420.43928822366378534No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87810.28696547329204725No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75490.24670337750616841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71950.23513456102223895No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA43580.14242062778803574No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.11392343012140721No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG34740.11353126685076553No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33590.10977303550711613No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA33030.10794294024412164No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT31630.1033677020866354No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC31230.10206049118449648No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31220.10202781091194299No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC30920.10104740273533881No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7150.055.7693141
TAACGCA8150.042.289884
TAGTACT11350.036.1259545
CATGGGT52850.035.64354
ATAGTAC11600.035.3473784
TACAACG6800.034.085642
CATGGGG95550.033.8325164
GCATAGT11850.033.6025472
AACGCAG443850.031.583326
GTATAAC10950.031.5239351
ACATGGG426150.030.9986673
TACATGG465050.029.6485942
GTACATG464850.029.639081
GTATCAA458750.029.4883121
CATGGGC86700.029.3353164
TTCAACG19700.029.2598154
CGCAGAG495700.028.3756268
ACGCAGA495000.028.2596867
GTACTGG19650.028.1674121
ACAACGC7950.027.656723
Produced by FastQC (version 0.11.5)