FastQCFastQC Report
Fri 10 Feb 2017
SRR3558045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584940
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT676521.8871166602509388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301140.8400140588126997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243120.6781703459472125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135750.37866742539624093No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA83710.23350460537693796No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA82330.22965516856628004No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT78890.2200594710092777No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.20251384960417745No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54740.15269432682276413No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA54080.1508532918263625No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA53660.14968172410137967No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA51850.14463282509609646No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50410.14061602146758384No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43640.12173146551964609No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC36230.10106166351459159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9000.060.1779371
GCATAGT13950.040.0590822
AACGCAG668750.037.522066
ATAGTAC14800.036.945234
TAGTACT14750.036.264595
TACAACG7550.033.8585932
AGATTAC15300.033.8045542
CATGGGT56900.033.738254
GTATCAA730050.033.579761
CGCAGAG751450.033.4242488
ACGCAGA750950.033.367367
TAACGCA10950.033.1091464
TATCACG7100.032.6552962
GCAGAGT780850.032.1657879
CATGGGG129850.031.8566114
TCACGCA7900.030.8453034
TATCAAC807200.030.203412
ACATGGG604900.029.466273
ATCAACG823000.029.420733
GTACATG638050.029.2522831
Produced by FastQC (version 0.11.5)