FastQCFastQC Report
Fri 10 Feb 2017
SRR3558044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6769035
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT923291.3639905835913095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440150.6502403961569115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348960.5155240000975029No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175310.2589881718738343No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA159140.23509998101649646No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT157590.23281014206604045No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC100450.14839633714406852No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT99640.14719971162802378No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94970.1403006484676176No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC91160.13467207659585154No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA82970.12257286304473239No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC82290.12156828853743555No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG71340.10539168433905276No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA71010.10490417023992342No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT68650.10141770577342264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG982550.032.8890766
GTATCAA1044650.031.0311851
ACGCAGA1089050.029.6127787
CGCAGAG1093450.029.5153548
CATGGGG195450.028.6102124
ACGGTAC17500.028.5238633
CGCATAG9850.028.3918511
GCAGAGT1142250.028.2699859
TACTGGT50100.027.5223032
GTACTGG60650.027.1757181
TATCAAC1190500.027.0835022
GGACCGT23800.026.9658366
ATCAACG1202550.026.6251183
TCAACGC1221800.026.4390854
CAACGCA1229700.026.3272235
AGTACTT557800.026.04758512-13
ACATGGG810600.026.0102233
GACCGTT14150.025.6177227
TACATGG911750.024.7383372
CATGGGT73900.024.6865864
Produced by FastQC (version 0.11.5)