FastQCFastQC Report
Fri 10 Feb 2017
SRR3558033.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558033.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775299
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197310.7109504237201109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83180.29971545408260514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76780.2766548757449197No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC73130.2635031396617085No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC59740.21525608592083229No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58250.20988729502658993No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT56260.20271689644971586No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA54490.19633920525319976No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT54410.19605094802397868No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT47470.17104463338905104No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT43900.1581811545350609No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC35720.12870685284720673No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34520.12438299440889072No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT33060.11912229997560622No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA30860.11119522617202687No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC30810.1110150654037637No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT29930.10784423588233195No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT28300.1019709948369527No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA28240.10175480191503691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4500.042.3010861
GACCGTT5950.039.9480177
GTATCAA272450.034.8684121
CATGGGT37900.033.7094354
TTAGGAC8400.032.5409853
AACGCAG298500.032.2893036
GTACTGG28100.031.9657381
CATGGGG96200.031.6262054
TAGTACT6650.030.381525
TATCAAC316250.029.7275712
ATAGTAC7000.029.7113364
ATCAACG316800.029.5425223
TCAACGC321550.029.4572374
TAACGCA4450.029.37754
ACATGGG300900.029.3262443
CAACGCA326900.029.0842085
TACTGGT26000.028.799222
ACGCAGA336700.028.6083037
CGCAGAG340900.028.3604268
ATGGGTG22250.028.042165
Produced by FastQC (version 0.11.5)