FastQCFastQC Report
Fri 10 Feb 2017
SRR3558032.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558032.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963472
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282980.7139699738007484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132310.33382347598267376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104180.2628503493906353No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92790.2341129191779329No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT81920.20668746997581916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.1429554693460683No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44340.11187161155673611No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41660.10510986327139438No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40950.10331850458385981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7150.044.1056821
TAACGCA8150.038.6392754
TACAACG5850.037.5885122
GTATAAC13100.030.431841
TATCACG6850.028.6307452
CATGGGG96050.028.4558224
AACGCAG411350.026.8810066
ACATGGG385200.026.777753
GTACATG435600.025.6253411
ATAACGC13250.025.5605183
TACATGG434850.025.516092
GTATCAA422850.025.2441541
ACGCAGA452450.024.4260397
CGCAGAG458050.024.27018
ACAACGC9000.023.7667983
CATGGGC91150.023.4669424
CATGGGT42050.023.3146344
GCAGAGT484650.022.9502969
CAACGCA478650.022.5800695
AGTACTT220100.022.54120312-13
Produced by FastQC (version 0.11.5)