FastQCFastQC Report
Fri 10 Feb 2017
SRR3558031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4925281
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394850.8016801477925828No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT152510.3096473074328145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147030.2985210386980966No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA144040.2924503190782414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137510.2791921922830393No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG84790.17215261423662934No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC80520.16348305812399333No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC71150.14445876286043374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68780.13964685466676927No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG63940.1298200041784418No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT55080.11183118282997458No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG53700.10902931223619526No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC53470.1085623338038987No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50440.10241040054364411No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT49680.10086734137605549No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49320.1001364186124609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA621450.033.140431
GGACCGT13850.030.892276
AACGCAG676350.030.7425256
GACCGTT10100.030.5949027
TATCAAC707950.028.9894832
ATCAACG715150.028.5344983
CAACGCA724650.028.4556355
TCAACGC722050.028.442884
CATGGGT71300.028.0871374
ACGCAGA744650.027.866937
CATGGGG182650.027.7196064
CGCAGAG751300.027.667738
ACGGTAC12750.027.4984953
GCAGAGT783950.026.4777899
CGCTAAC6200.024.96642362-63
GAGTACT242650.024.34319712-13
AGTACTT276750.024.23178712-13
GTACTGG39800.024.2211441
TAACGCA8100.024.210074
TATCACG8150.024.0659392
Produced by FastQC (version 0.11.5)