FastQCFastQC Report
Fri 10 Feb 2017
SRR3558029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4230570
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT605581.4314383168225557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287320.6791519818842378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233710.5524314690455423No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119740.28303514656417456No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99950.23625658008258935No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT95730.22628156489551055No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA65480.15477819773694798No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59820.14139938589835413No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA57540.13601004120012197No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC54230.1281860363969867No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53370.12615321339677632No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA52100.12315125384995403No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC47700.11275076408143585No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA46200.109205142569441No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA45200.10684139489477779No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42850.10128658785931918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8050.055.433571
AACGCAG679200.034.8899236
GTATCAA702300.033.108491
TAGTACT13700.032.5299385
TATCACG8050.032.4868162
ATAGTAC13900.032.0618824
TAACGCA8800.031.7364064
AGATTAC16800.031.486982
ACGGTAC9350.031.14063
ACGCAGA759950.031.1122517
CGCAGAG763450.031.07088
TCACGCA8800.031.0611634
GATTACT16650.030.6921083
GCAGAGT799300.029.6995269
GCATAGT15150.029.4238152
TATCAAC797350.029.0788542
ATCAACG808950.028.5225453
CATGGGG141350.028.4600324
TCAACGC826550.028.3393614
CAACGCA833000.028.176995