FastQCFastQC Report
Fri 10 Feb 2017
SRR3558026.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558026.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5607072
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569621.015895640362742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268000.4779678234914765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209640.3738849795401236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116690.20811218404186713No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109640.19553877674479658No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT104250.18592591641412845No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA61880.11036063028974838No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59110.10542044047231781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9650.039.4611131
AACGCAG637550.035.140276
GTATCAA649700.033.774951
ACGCAGA713750.031.3637167
CGCAGAG716700.031.3258298
CATGGGT68750.030.5126534
ACATGGG598500.030.1053073
TATCAAC732200.029.888872
ATCAACG735050.029.5898113
GCAGAGT759800.029.5332159
TCAACGC747700.029.5263294
GTACATG644650.029.4338571
TACATGG645800.029.4330922
CAACGCA759050.029.1866025
CATGGGG154750.028.8010674
TACAACG10050.027.2049062
CATGGGC142800.026.9248984
TAACGCA10750.026.534494
TATCACG8600.025.571652
AGTACTT388550.024.9374412-13
Produced by FastQC (version 0.11.5)