FastQCFastQC Report
Fri 10 Feb 2017
SRR3558017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157010
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118921.0278217128633287No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59200.5116636848428276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49700.4295554921737928No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47900.41399815040492305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43540.37631481145366075No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT31430.27164847321976476No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25740.22246998729483755No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25270.21840779249963269No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24500.21175270740961616No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21360.1846137889905878No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21150.18279876578421966No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC17580.15194337127596133No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA16480.142436106861652No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15030.1299038037700625No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14860.12843449926966924No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA12440.10751851755818878No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12200.10544420532233947No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC11690.10103629182115971No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11670.10086343246817227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3200.055.7915341
ACGCATA752.309389E-855.543571
ACAACGC2650.051.584073
TATCACG2450.050.9520762
TACAACG2750.049.7168772
TCACGCA2800.046.6979984
TAACGCA3600.042.924424
ATCACGC3100.042.1788333
GTATCAC3550.041.909131
GTACAAC4300.038.7513281
GTATAAC4500.037.029051
CATGGGG37000.036.7847144
GACCGTT2300.036.1771057
ATAGTAC5050.032.9534034
ATAACGC5100.032.630333
CATGGGT14800.032.527974
GGACCGT3000.031.6980366
GCATAGT5450.031.6308022
AGATTAC5400.030.822862
TAGTACT5450.030.5348035
Produced by FastQC (version 0.11.5)