FastQCFastQC Report
Fri 10 Feb 2017
SRR3558008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4146429
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT527371.2718655016159688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269240.6493298209133691No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205800.49633069805367463No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA134340.32398963059538705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122610.2957002278346018No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT107470.2591868810487289No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA78210.18862013554313845No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG69580.16780704553243284No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56910.13725063180872024No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT56420.13606889205144956No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG56250.13565890070709036No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.12483030578842663No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50480.12174331213678083No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA47410.11433935080041163No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG46460.11204822269958077No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44030.10618775818903446No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.10481308132853596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG12750.066.72991
TAGTACT16300.052.1431925
TAACGCA11650.052.0370864
ATAGTAC18500.045.6200074
GCATAGT18950.044.8584372
TACAACG8950.041.8441542
AGATTAC23700.041.636772
GTATAAC15850.040.9158741
GATTACT25600.038.539573
ATAACGC19350.036.8586163
TATCACG7200.034.6764072
ACAACGC10650.034.0423053
CATGGGG125550.033.279544
GTACAAC13100.033.154781
ACGCATA4350.031.4581491
CATAGTA26750.031.3280983
AACGCAG674100.029.2109936
CATGGGT51250.028.8764744
ATTACTT34750.028.5627754
CATGGGC122350.028.1749234
Produced by FastQC (version 0.11.5)