FastQCFastQC Report
Fri 10 Feb 2017
SRR3558000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3558000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754558
Sequences flagged as poor quality0
Sequence length125
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185462.457862748788032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99421.317592550870841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87111.154450685036803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43870.5813999719040815No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39570.5244129675916231No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34570.45814900908876455No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29060.38512612681861436No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA24410.3235006454109558No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22390.2967300061758009No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20160.267176280683526No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18250.24186344853543398No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.2410682810333997No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.23669485977221102No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT17840.2364298039381996No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16760.2221167889015821No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.2088639972010104No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC15310.20290024093575312No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14900.1974665963385187No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA14400.19084020048823286No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13720.18182830213184406No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA13520.17917774379172974No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA13120.17387662711150104No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA12170.16128647499595788No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12110.1604913074939236No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA11820.15664799790075778No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT11310.14988907413346622No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA10920.14472048537024323No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10660.1412747595280946No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10280.13623869868187732No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA10070.13345561242475726No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.12484129781938566No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9410.12470876990237993No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT9250.12258832323028847No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.12258832323028847No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8890.11781731821808264No Hit
GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8780.11635951113101975No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.11317884112288254No Hit
GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.11026322694875676No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA8260.10946805944672246No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.1081427802766653No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGGTGGAAAA8050.10668497318960239No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8020.10628738943858525No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7950.10535969401954523No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC7820.10363683109847088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5200.090.412981
TACAACG2600.077.730992
GTATAAC3050.072.19531
GCATAGT6600.072.050122
GAGTTCT6100.071.219691
ATAGTAC6700.070.073634
GTACAAC3150.068.01411
TAGTACT6850.067.6760565
ATAACGC3850.066.375833
CATAGTA7150.065.663393
AACGTAG551.72422E-764.922561
GGTTTCA4700.063.3110051
AGTTCTT7000.060.290512
CGCGAGT401.7697731E-459.5123441
ACGCGGA507.4403524E-659.5123441
AGATTAC7900.056.4316562
TCACGCA2750.054.026844
ACAACGC3650.053.7308043
TATCACG2450.053.37592
GTGTACT5550.052.469314
Produced by FastQC (version 0.11.5)