FastQCFastQC Report
Fri 10 Feb 2017
SRR3557967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6243169
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1082231.7334626052890767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT495330.7933951491622283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405370.6493016607431258No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA204610.3277342003716382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198330.3176752062934705No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT177270.28394233761732224No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA131070.20994145761551544No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT110180.17648088654976343No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC104010.16659808504302864No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC98440.15767633392592767No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94470.15131738384785035No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT92550.148242022601022No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG87020.13938434150989665No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA77340.12387939522380381No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG74850.1198910361068233No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA71320.11423685631447747No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70750.11332385844432531No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA70280.11257103563911212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT12550.039.3003237
CGCATAG12750.037.3250961
GTATCAA1048000.035.7999731
AACGCAG1098050.034.6191186
TACAACG9450.032.073172
GGACCGT23700.031.8432336
TATCAAC1176850.031.7638442
TTAGGAC18400.031.6497333
ACGGTAC17650.031.64793
ATCAACG1189450.031.344413
CGCAGAG1224550.031.1059388
ACGCAGA1224600.031.0852577
TAACGCA11100.031.050374
TCAACGC1214350.031.0393494
CAACGCA1228200.030.713525
CATGGGG215000.030.5964414
CATGGGT84300.030.5931324
GCAGAGT1272350.029.9046489
CATGGGC228850.029.6535664
GTACTGG51800.028.709881