FastQCFastQC Report
Fri 10 Feb 2017
SRR3557947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5884636
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT655111.1132549234990916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315420.5360059653647226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247510.4206037552705044No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA223920.3805163140082071No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT181390.30824336458533713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132330.22487372201101308No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG105960.18006211429220093No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC88310.15006875531468727No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG87680.1489981708299375No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT85410.14514066800393433No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA82310.1398727126027846No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG74210.126108054941716No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA72150.12260741361062943No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC64540.10967543277103291No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC62830.10676956059814066No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60820.10335388628965325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG12000.061.9637371
TACAACG11450.042.041542
TAACGCA14150.040.3162964
ACAACGC13300.038.424893
TATCACG8900.037.393582
TAGTACT21800.036.254425
ATAGTAC22450.034.940044
TCACGCA11550.030.8698944
AGATTAC26250.030.5635282
CATGGGT98000.030.2580074
GTATAAC21450.029.9505861
GCATAGT25600.029.714542
CATGGGG168450.028.6451324
GTACAAC18950.028.5654491
AACGCAG929550.028.4033376
ACGGTAC12150.027.878183
ATCACGC12850.027.2844183
ATAACGC29650.026.6558653
GTATCAA962750.026.5559441
AAACGCA16950.026.2940445